«ΔΟΜΗ ΚΑΙ...

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ΕΙΔΙΚΑ ΚΕΦΑΛΑΙΑ ΚΥΤΤΑΡΙΚΗΣ ΒΙΟΛΟΓΙΑΣ. Διάλεξη:. «ΔΟΜΗ ΚΑΙ ΛΕΙΤΟΥΡΓΙΑ ΤΗΣ ΕΡΥΘΡΟΚΥΤΤΑΡΙΚΗΣ ΜΕΜΒΡΑΝΗΣ». Παρουσίαση: Δρ. Μ.Χ. Αντωνέλου, 07 /0 5 /0 8. http://kyttariki.biol.uoa.gr , http://multimedia.biol.uoa.gr. Ιστοσελίδες μαθημάτων. ΕΚΚΒ_2008_Ερυθροκυτταρική Μεμβράνη_Αντωνέλου. - PowerPoint PPT Presentation

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:: . .. , 07/05/08_2008_ _http://kyttariki.biol.uoa.gr, http://multimedia.biol.uoa.gr
Triton X-100 _2008_ _
2D-structure_2008_ _
I (65%) _2008_ _
// :Sp-ank (band 3) (***)Sp-4.1R-actin-p55-GpCRh, RhAG-ankSp, 4.1R PS_2008_ _
_2008_ _ , (Palek & Sahr,1992)
_2008_ _
E, , , , , , , ()Head-to-head of 2 () tetramer 223-6 nm, 200-260 nm
_2008_ _
, 2 (chr1), 4 -, 1 - (chr14) (human) , Golgi homo--E, Sp Sp ,
_2008_ _
: 29-37 nm in situ, 100 nm : 200-260 nm_2008_ _
, ()_2008_ _
-domains (HE)_2008_ _
O , . in vitro oC [Sp]In vivo (37oC) Sp [Sp]T TSp Sp Sk (HE)
- . , (, ), . _2008_ _
_2008_ _
pH Sp- Sp-Ank [Sp] , Sp-actin (-) 4.1R Sp: ank _2008_ _
- () 33-37 nm ( , ) (G) SkA 4.1R SpM 4.1R extra 4.1R, Sp
_2008_ _
4.1R Sp-actin, GpC-p55 200.000 /RBCA 4.1 familyE, , , , , , &
_2008_ _
4.1R 4.1R calmodulin/Ca++ ( & Sp/actin).H Ca++ calmodulin (20x) calmodulin calmodulin 4.1. 4.1 p55, GpC, B3
4.1RA -3 domains: . (PS, B3, p55, calmodulin) . Sp/actin (, ) III. COOH domain ( )General Brain Neuron( mRNA)RBC_2008_ _
4.1RGhosts (Laemmli-linear 5-15%) RBC
SDS-PAGE: 4.1/, 80 & 78 kDa
- Asn502 .
4.1/: ,
.
4.1(-) _2008_ _
Sp-actin ( ) : 3 / (, , )RBC: , 15.000 /RBC ( )
( Sp). Sp/actin_2008_ _
_2008_ _p55 MAGUKs (Membrane-associated Guanylate kinase homologues) Sk , , cell proliferation, RBC: SDS-PAGE band 4.94.1-GpC ( , )(-)4.1R, (-)GpC p55
Z 4.9 (48, 52 kDa), 43.000/RBC _2008_ _
()Actin-binding protein2 33-37nm SpT4 (, , )RBC: 29/27 kDa, 70-80.000 copies/RBC : 5 () TM5b () - 1) 6 , 2) D/actin_2008_ _
(TD) 40.6 kda ( RBC)I .:1) 2) /
_2008_ _
SUNG et al., 2000_2008_ _
- (215 kDa), (4 domains)Sp (T)10x_2008_ _
(-) ank band 3 dimersM - , , _2008_ _
(Restricted, Broadly, Giant) ,, .(2.400) _2008_ _
(4.2)72 kDa, 200.000 copies/RBC -myristylated (myristic acid) (palmitic acid) (b3,p55) (-) exon , , , PLTT-Hereditary spherocytosis-like (hemolytic anemia, .RBC)Band 3 (* 3-Sk, ), ank, 4.1R(?), _2008_ _
O --
AYTO : Ca++/calmodulin/calpainPhosphorylation- : - 4.2-
_2008_ _
Sk
Sp-Ank, , Golgi_2008_ _
GOLGI , , 2D- Golgi _2008_ _
, RBC (nucl., TfR, microtub, membr, Sk, S/V) RBC Sk / Sk ( Sk ) (?) Sk (-) .
/ - (akn)3. (4.1R, TM5-TD)_2008_ _
(2-3x Sp/) . Sp, ank / - (, 10% )Band 3, 4.1 membrane-associated system Sp, actin, ank, , Sk Band 3, 4.1 (isoforms) in situ b3/Sp/ank.(-/-)1 mouse Sk _2008_ _
_2008_ _