«ΔΟΜΗ ΚΑΙ...

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ΕΙΔΙΚΑ ΚΕΦΑΛΑΙΑ ΚΥΤΤΑΡΙΚΗΣ ΒΙΟΛΟΓΙΑΣ. Διάλεξη:. «ΔΟΜΗ ΚΑΙ ΛΕΙΤΟΥΡΓΙΑ ΤΗΣ ΕΡΥΘΡΟΚΥΤΤΑΡΙΚΗΣ ΜΕΜΒΡΑΝΗΣ». Παρουσίαση: Δρ. Μ.Χ. Αντωνέλου, 07 /0 5 /0 8. http://kyttariki.biol.uoa.gr , http://multimedia.biol.uoa.gr. Ιστοσελίδες μαθημάτων. ΕΚΚΒ_2008_Ερυθροκυτταρική Μεμβράνη_Αντωνέλου. - PowerPoint PPT Presentation

Transcript of «ΔΟΜΗ ΚΑΙ...

  • :: . .. , 07/05/08_2008_ _http://kyttariki.biol.uoa.gr, http://multimedia.biol.uoa.gr

  • Triton X-100 _2008_ _

  • 2D-structure_2008_ _

  • I (65%) _2008_ _

  • // :Sp-ank (band 3) (***)Sp-4.1R-actin-p55-GpCRh, RhAG-ankSp, 4.1R PS_2008_ _

  • _2008_ _ , (Palek & Sahr,1992)

  • _2008_ _

  • E, , , , , , , ()Head-to-head of 2 () tetramer 223-6 nm, 200-260 nm

    _2008_ _

  • , 2 (chr1), 4 -, 1 - (chr14) (human) , Golgi homo--E, Sp Sp ,

    _2008_ _

  • : 29-37 nm in situ, 100 nm : 200-260 nm_2008_ _

  • , ()_2008_ _

  • -domains (HE)_2008_ _

  • O , . in vitro oC [Sp]In vivo (37oC) Sp [Sp]T TSp Sp Sk (HE)

  • - . , (, ), . _2008_ _

  • _2008_ _

  • pH Sp- Sp-Ank [Sp] , Sp-actin (-) 4.1R Sp: ank _2008_ _

  • - () 33-37 nm ( , ) (G) SkA 4.1R SpM 4.1R extra 4.1R, Sp

    _2008_ _

  • 4.1R Sp-actin, GpC-p55 200.000 /RBCA 4.1 familyE, , , , , , &

    _2008_ _

  • 4.1R 4.1R calmodulin/Ca++ ( & Sp/actin).H Ca++ calmodulin (20x) calmodulin calmodulin 4.1. 4.1 p55, GpC, B3

  • 4.1RA -3 domains: . (PS, B3, p55, calmodulin) . Sp/actin (, ) III. COOH domain ( )General Brain Neuron( mRNA)RBC_2008_ _

  • 4.1RGhosts (Laemmli-linear 5-15%) RBC

    SDS-PAGE: 4.1/, 80 & 78 kDa

    - Asn502 .

    4.1/: ,

    .

    4.1(-) _2008_ _

  • Sp-actin ( ) : 3 / (, , )RBC: , 15.000 /RBC ( )

  • ( Sp). Sp/actin_2008_ _

  • _2008_ _p55 MAGUKs (Membrane-associated Guanylate kinase homologues) Sk , , cell proliferation, RBC: SDS-PAGE band 4.94.1-GpC ( , )(-)4.1R, (-)GpC p55

  • Z 4.9 (48, 52 kDa), 43.000/RBC _2008_ _

  • ()Actin-binding protein2 33-37nm SpT4 (, , )RBC: 29/27 kDa, 70-80.000 copies/RBC : 5 () TM5b () - 1) 6 , 2) D/actin_2008_ _

  • (TD) 40.6 kda ( RBC)I .:1) 2) /

    _2008_ _

  • SUNG et al., 2000_2008_ _

  • - (215 kDa), (4 domains)Sp (T)10x_2008_ _

  • (-) ank band 3 dimersM - , , _2008_ _

  • (Restricted, Broadly, Giant) ,, .(2.400) _2008_ _

  • (4.2)72 kDa, 200.000 copies/RBC -myristylated (myristic acid) (palmitic acid) (b3,p55) (-) exon , , , PLTT-Hereditary spherocytosis-like (hemolytic anemia, .RBC)Band 3 (* 3-Sk, ), ank, 4.1R(?), _2008_ _

  • O --

  • AYTO : Ca++/calmodulin/calpainPhosphorylation- : - 4.2-

    _2008_ _

  • Sk

  • Sp-Ank, , Golgi_2008_ _

  • GOLGI , , 2D- Golgi _2008_ _

  • , RBC (nucl., TfR, microtub, membr, Sk, S/V) RBC Sk / Sk ( Sk ) (?) Sk (-) .

    / - (akn)3. (4.1R, TM5-TD)_2008_ _

  • (2-3x Sp/) . Sp, ank / - (, 10% )Band 3, 4.1 membrane-associated system Sp, actin, ank, , Sk Band 3, 4.1 (isoforms) in situ b3/Sp/ank.(-/-)1 mouse Sk _2008_ _

  • _2008_ _