Post on 18-Apr-2018
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Supplementary Materials
Therapeutic targeting of the oncostatin M receptor-β prevents inflammatory heart failure
Jochen Pöling1,2*; Praveen Gajawada1*, Manfred Richter3, Holger Lörchner1, Silvia
Schimanski1, Victoria Polyakova1,3, Sawa Kostin1, Jaeyoung Shin1, Thomas Boettger1,
Thomas Walther3, Wolfgang Rees2; Astrid Wietelmann1, Henning Warnecke2,4, Thomas
Kubin1# and Thomas Braun1#
1 Department of Cardiac Development and Remodelling, Max Planck Institute
for Heart and Lung Research, Ludwigstrasse 43, Bad Nauheim, 61321, Germany
2 Department of Cardiac Surgery, Schüchtermann-Clinic, Ulmenallee 5,
Bad Rothenfelde, 49214, Germany
3 Department of Cardiac Surgery, Kerckhoff Hospital, Benekestrasse 2-8,
Bad Nauheim, 61231, Gerrmany
4 University of Witten/Herdecke, Alfred-Herrhausen-Straße 50, 58448, Germany
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Suppl. Fig. S1: Increased Oβ/gp130 signaling but unstable activation of Erk1/2 during
early stages of DCM in MCP-1 mice. (A) Western blot analysis of Oβ/gp130 and Erk1/2
signaling in wild-type and MCP-1 transgenic mouse hearts at 2 to 4 weeks after birth (n=4 per
time point). (B) Western blot analysis of Oβ/gp130 and Erk1/2 signaling in 2 months old wild
type and MCP-1 mouse hearts. No significant increase of Erk1/2 phosphorylation is
detectable at this age despite increased Oβ/gp130 signaling (n=4). (C) Western blot analysis
of increased Erk1/2 phosphorylation in 6 months old MCP-1 but not wild type hearts (n=5 for
each group). (D) Inhibition of Oβ signaling by antibodies against Oβ or removal of a single
allele of Oβ leads to decreased Erk1/2 phosphorylation in 6 month old MCP-1 mice (n=5 for
each group).
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Suppl. Fig. S2: Reduced apoptosis of cardiomyocytes in 6 months old MCP-1 mice after
treatment with antibodies against Oβ or removal of a single allele of Oβ. Confocal
immunofluorescence analysis of hearts from wild type, MCP-1, anti-Oβ antibody treated
MCP-1 and MCP-1/Oβ(+/-) mice after TUNEL-staining. Cardiomyocytes are identified by
staining with antibodies against sarcomeric actinin (green). A statistical analysis is shown
(n=3 for each group).
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Supplementary Movie S1. MRI Analysis of Heart Functions: (A) Cine MRI analysis of the
heart function of MCP-1 transgenic mice at the age of 4 months before treatment with a
isotype matched control antibody or with anti-Oβ antibody. (B) Cine MRI analysis of the
heart function of MCP-1 transgenic mice at the age of 6 months after treatment with a control
antibody or with anti-Oβ antibody. Please note improved heart function of MCP-1 mice
treated with anti-Oβ antibody.