Slide Tambahan

Post on 31-Jan-2016

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Transcript of Slide Tambahan

O

HH

HO

H

HO

H

O

HH

HO

H

H

H

O

HH

HO

H

H

O

HH

OH

HO

H

H

OH

OOH

OH

OH

O

OH

OH

O

HOH

H

Manan

O

HH

HO

H

HO

H

O

HH

HO

H

H

H

O

HH

HO

H

H

O

HH

OH

HO

H

H

OH

OOH

OH

OH

O

OH

OH

O

HOH

H

OH

H

H

O

HH

HO

H

HO

H

O

HH

HO

H

H

H

OH

OOH

OH

OH

H

OH

O

HH

OH

HO

H

H

HOH

OHHO

Oligomanan

Manobiosa

Manosa

Serat manan

Hidrolisis Protein

C

R1

H

H2N C

O

NH C C OH

H O

R2

H2O C

R1

H

H2N C

O

OH + C

R2

H

HN C

O

OHH

Dipeptida Asam amino 1 Asam amino 2

α-D-galaktosidase

β-mananaseβ-mananase

Hidrolisis galaktomanan oleh mananase

+

Reaksi gula pereduksi dengan DNS

O

H

H

HO

OH

H

HH

OH

O

H

O

H

HO

OH

H

HHO

O

O

H

H

HO

OH

H

HH

OH

O

H

O

H

HO

OH

H

HH

OH

O

OH

H

OH

HO

H

OHHH

OH

OH

O

OH

H

H

HO

H

OHHH

OH

Galaktomanan

Galaktosa

Hidrolisis galaktomanan menjadi galaktosa

O

H

H

HO

OH

H

HH

OH

O

H

O

H

HO

OH

H

HHO

OH

O

H

H

HO

OH

H

HH

OH

O

H

O

H

HO

OH

H

HH

OH

OH

O

H

HO

H

HO

H

OHHOH

OH

Glukomanan

Glukosa

Hidrolisis glukomanan menjadi glukosa

OHOH2C

O

OHOH2C

O OH2C

OO

HOH2C

OO

HOH2C

O OH2C

OO

OHO

H2CO

O O O

H2C

O

OHOH2C

OHOH2C

OO

HOH2C OHOH2C

-D-galaktosidase

ekso--D-mananase

endo--D-mananase

OHOH2C

O

OHOH2C

O OH3C

OO

HOH2C

OO

HOH2C

O OH3C

OO

OHO

H3CO

H2C

O

OHOH2C

OHOH2C

OO

HOH2C OHOH2C

-D-galaktosidase

manosa

OHOH2C

OHOH2C

OHOH2C

-D-manosidase

manobiosa

Hidrolisis serat oleh enzim mananase

HO

NH2

OH

O

Struktur L-tirosin

H3PO4(MoO3)13 +

OH

+ H2O

O

O

+ H3(PMo13O40)

Pereaksi Folin Ciocalteu

Fenol

Kuinon

Kompleks molibdenum biru

Reaksi fenol dengan reagen Folin Ciocalteu