Langmuir technology

Langmuir technology

STM photo of Langmuir film of Stearic AcidC17H35COOH

Langmuir technology

Type of polymer

Area per monomeric chain containing cetyl-radical, A2

Area per general monomeric chain, A2

abPEI-12 alPEI-35 alPEI-70 abPEI-70 aP4VP-70

cLMMA-25 cLMMA-40 cLMMA-90

31.0 50.0 32.0 55.0 17.0 76.0 56.0 28.0

6.2 14.9 23.4 37.9 11.4 19.6 19.2 20.0

Schemas of STM and AFM a – scanning tunneling microscopyb – atomic force microscopy

AFM images of Langmuir films of polymers

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Langmuir film of antibodies with polymer PEI-R12 on graphite surface

Langmuir film of antibodies with polymer PEI-L70 on graphite surface. AFM image, size 2,6x2,6 μ.

AFM images of Langmuir films of polymers 

 

 

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a b

c d

AFM images of Langmuir films of polymer PEI-R12, size1,51,5 μ.

a – surface pressure by LB process í=10 mN/m, thickness of film 2,5 nm;

b – í=20 mN/m, thickness of film 3,5 nm;

c – í=30 mN/m, thickness of film 3,5 nm;

d – í=40 mN/m, thickness of film 3,5 nm.

AFM images of Langmuir films of polymers

 

AFM images of Langmuir films of polymer PEI-L70, size1,51,5 μ.

a – surface pressure by LB process í=10 mN/m, thickness of film 3,3 nm;

b – í=20 mN/m, thickness of film 4 nm;

c – í=30 mN/m, thickness of film 5,5 nm;

d – í=40 mN/m, thickness of film 6 nm.

a b

c d

STM images of DNA

DNA molecules on graphite surface 

AFM images of viruses

Sample of rotaviruses specific adsorbed on Langmuir film from antibodies with polymer PEI-R12. AFM image, 3D projection with highlight.

 Viruses of  Variolovaccine on mica surface

Cells of Rickettsia bacterium C. burnetii (culture ”Yellow throat mouse -Luga”) on Langmuir film from antibodies with polymer PEI-L70. 3D projection with highlight.

 Cells of bacterium Rickettsia prowazekii (culture ”Breil”) on Langmuir film from antibodies with polymer PEI-R12. 3D projection with highlight. 

Cells of bacterium Rickettsia bacterium C. burnetii (culture ”M-44”) onmica. 3D projection with highlight.

AFM images of bacteriums

Cells of bacterium Yersinia pestis (culture ”EB”) on Langmuir film from antibodies with polymer PEI-R12. 3D projection with highlight.

AFM images of bacteriums

ASM images of Bacillus subtilis

   AFM images of bacteriums

AFM images of bacteriums

ASM images of Staphylococcus aureus 209-P

 

AFM images of bacteriums

Bacteriums Staphylococcus aureus on glass. ASM image with highlight.

     

Bacteriums Staphylococcus aureus, treated for 15 min by enzyme lysoamidaze. ASM image with highlight.

Investigations of 2D crystalline films of ferritin

1- initial electronmicroscopic photo 2 - optical diffraction 3 - after Fourier filtration

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2

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Investigations of 2D crystalline films of protein

Self-organized protein of S-layer of Bac.spaericus cell wall with tetragonal packing of sub-units. TEM, contrast by 1% uranyle-acetate.

1 - fragment EM photo,

2 - optical diffraction pattern,

3 - filtered image

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