ENO1 Polyclonal antibodyCatalog Number:11204-1-AP Featured Product 36 Publications

For Research Use Only

www.ptgcn.com

Basic Information Catalog Number:11204-1-AP

Size:700 μg/ml

Source:Rabbit

Isotype:IgG

Purification Method:Antigen affinity purification

Immunogen Catalog Number:AG1692

GenBank Accession Number:BC015641

GeneID (NCBI):2023

Full Name:enolase 1, (alpha)

Calculated MW:47 kDa

Observed MW:47 kDa

Recommended Dilutions:WB 1:2000-1:12000 IP 0.5-4.0 ug for IP and 1:500-1:1000for WBIHC 1:100-1:400 IF 1:50-1:500

Applications Tested Applications:FC, IF, IHC, IP, WB,ELISA

Cited Applications:IF, IHC, IP, WB

Species Specificity:human, mouse, rat

Cited Species:human, mouse, rat

Note-IHC: suggested antigen retrieval withTE buffer pH 9.0; (*) Alternatively, antigenretrieval may be performed with citratebuffer pH 6.0

Positive Controls:

WB : HeLa cells; human skeletal muscle tissue, SGC-7901 cells, mouse skeletal muscle tissue, PC-3 cells,L02 cells, mouse liver tissue

IP : mouse skeletal muscle tissue;

IHC : human colon cancer tissue; human liver cancertissue, human stomach cancer tissue

IF : HepG2 cells;

Background Information Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate tophosphoenolpyruvate.Enolase were down-regulated in oxLDL-treated cells or in VLDL-treated cells .And it identifiedwhich are associated with glucose metabolism were down-regulated in the process of foam cells formation.(19756395)

Notable Publications Author Pubmed ID Journal Application

Bing Sun 27684953 PLoS One WB

Si Gao 28910549 Can J Physiol Pharmacol WB

Xixi Guo 31480692 Biomolecules WB

Storage Storage:Store at -20ºC. Stable for one year after shipment.Storage Buffer:PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Aliquoting is unnecessary for -20ºC storage

T: 4006900926 E: Proteintech-CN@ptglab.com W: ptgcn.comFor technical support and original validation data for this product please contact: This product is exclusively available under Proteintech

Group brand and is not available to purchase from anyother manufacturer.

Selected Validation Data

Various lysates were subjected to SDS PAGEfollowed by western blot with 11204-1-AP (ENO1antibody) at dilution of 1:6000 incubated at roomtemperature for 1.5 hours.

IP Result of anti-ENO1 (IP:11204-1-AP, 3ug;Detection:11204-1-AP 1:500) with mouse skeletalmuscle tissue lysate 3500ug.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using11204-1-AP (ENO1 Antibody) at dilution of 1:200(under 10x lens). Heat mediated antigen retrievalwith Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using11204-1-AP (ENO1 Antibody) at dilution of 1:200(under 40x lens). Heat mediated antigen retrievalwith Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20℃ Ethanol)fixed HepG2 cells using 11204-1-AP (ENO1antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 HeLa cells were stained with 0.2ug ENO1antibody (11204-1-AP, red) and control antibody(blue). Fixed with 90% MeOH blocked with 3% BSA(30 min). Alexa Fluor 488-conjugated AffiniPureGoat Anti-Rabbit IgG(H+L) with dilution 1:1500.