Immunofluorescence Antibody Customer Review for Laminin α-1 Polyclonal Antibody (STJ93888)
ENO1 Polyclonal antibody · 2021. 1. 26. · 11204-1-AP (ENO1 Antibody) at dilution of 1:200 (under...
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ENO1 Polyclonal antibody Catalog Number: 11204-1-AP Featured Product 36 Publications For Research Use Only www.ptgcn.com Basic Information Catalog Number: 11204-1-AP Size: 700 μg/ml Source: Rabbit Isotype: IgG Purification Method: Antigen affinity purification Immunogen Catalog Number: AG1692 GenBank Accession Number: BC015641 GeneID (NCBI): 2023 Full Name: enolase 1, (alpha) Calculated MW: 47 kDa Observed MW: 47 kDa Recommended Dilutions: WB 1:2000-1:12000 IP 0.5-4.0 ug for IP and 1:500-1:1000 for WB IHC 1:100-1:400 IF 1:50-1:500 Applications Tested Applications: FC, IF, IHC, IP, WB,ELISA Cited Applications: IF, IHC, IP, WB Species Specificity: human, mouse, rat Cited Species: human, mouse, rat Note-IHC: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 Positive Controls: WB : HeLa cells; human skeletal muscle tissue, SGC- 7901 cells, mouse skeletal muscle tissue, PC-3 cells, L02 cells, mouse liver tissue IP : mouse skeletal muscle tissue; IHC : human colon cancer tissue; human liver cancer tissue, human stomach cancer tissue IF : HepG2 cells; Background Information Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate.Enolase were down-regulated in oxLDL-treated cells or in VLDL-treated cells .And it identified which are associated with glucose metabolism were down-regulated in the process of foam cells formation. (19756395) Notable Publications Author Pubmed ID Journal Application Bing Sun 27684953 PLoS One WB Si Gao 28910549 Can J Physiol Pharmacol WB Xixi Guo 31480692 Biomolecules WB Storage Storage: Store at -20ºC. Stable for one year after shipment. Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20 º C storage T: 4006900926 E: [email protected] W: ptgcn.com For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech Group brand and is not available to purchase from any other manufacturer.
Transcript of ENO1 Polyclonal antibody · 2021. 1. 26. · 11204-1-AP (ENO1 Antibody) at dilution of 1:200 (under...
ENO1 Polyclonal antibodyCatalog Number:11204-1-AP Featured Product 36 Publications
For Research Use Only
www.ptgcn.com
Basic Information Catalog Number:11204-1-AP
Size:700 μg/ml
Source:Rabbit
Isotype:IgG
Purification Method:Antigen affinity purification
Immunogen Catalog Number:AG1692
GenBank Accession Number:BC015641
GeneID (NCBI):2023
Full Name:enolase 1, (alpha)
Calculated MW:47 kDa
Observed MW:47 kDa
Recommended Dilutions:WB 1:2000-1:12000 IP 0.5-4.0 ug for IP and 1:500-1:1000for WBIHC 1:100-1:400 IF 1:50-1:500
Applications Tested Applications:FC, IF, IHC, IP, WB,ELISA
Cited Applications:IF, IHC, IP, WB
Species Specificity:human, mouse, rat
Cited Species:human, mouse, rat
Note-IHC: suggested antigen retrieval withTE buffer pH 9.0; (*) Alternatively, antigenretrieval may be performed with citratebuffer pH 6.0
Positive Controls:
WB : HeLa cells; human skeletal muscle tissue, SGC-7901 cells, mouse skeletal muscle tissue, PC-3 cells,L02 cells, mouse liver tissue
IP : mouse skeletal muscle tissue;
IHC : human colon cancer tissue; human liver cancertissue, human stomach cancer tissue
IF : HepG2 cells;
Background Information Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate tophosphoenolpyruvate.Enolase were down-regulated in oxLDL-treated cells or in VLDL-treated cells .And it identifiedwhich are associated with glucose metabolism were down-regulated in the process of foam cells formation.(19756395)
Notable Publications Author Pubmed ID Journal Application
Bing Sun 27684953 PLoS One WB
Si Gao 28910549 Can J Physiol Pharmacol WB
Xixi Guo 31480692 Biomolecules WB
Storage Storage:Store at -20ºC. Stable for one year after shipment.Storage Buffer:PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Aliquoting is unnecessary for -20ºC storage
T: 4006900926 E: [email protected] W: ptgcn.comFor technical support and original validation data for this product please contact: This product is exclusively available under Proteintech
Group brand and is not available to purchase from anyother manufacturer.
Selected Validation Data
Various lysates were subjected to SDS PAGEfollowed by western blot with 11204-1-AP (ENO1antibody) at dilution of 1:6000 incubated at roomtemperature for 1.5 hours.
IP Result of anti-ENO1 (IP:11204-1-AP, 3ug;Detection:11204-1-AP 1:500) with mouse skeletalmuscle tissue lysate 3500ug.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using11204-1-AP (ENO1 Antibody) at dilution of 1:200(under 10x lens). Heat mediated antigen retrievalwith Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using11204-1-AP (ENO1 Antibody) at dilution of 1:200(under 40x lens). Heat mediated antigen retrievalwith Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (-20℃ Ethanol)fixed HepG2 cells using 11204-1-AP (ENO1antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
1X10^6 HeLa cells were stained with 0.2ug ENO1antibody (11204-1-AP, red) and control antibody(blue). Fixed with 90% MeOH blocked with 3% BSA(30 min). Alexa Fluor 488-conjugated AffiniPureGoat Anti-Rabbit IgG(H+L) with dilution 1:1500.
