Copenhagen, 5 March 2015

Identification of the most immunogenic/protective VLP-HA construct in mice

Department of Immunology

Development of a universal influenza vaccine based on tandem core technology

Schematic of VLP-HA constructs

Modified from Steel et al, mBio 2010

ΔHA stalk (HA292-553):

GGGG

VLP-HA constructsΔHA stalk (HA292-553):

HA292-351 :

HA306-474 :

HA403-474 :

LAH (HA420-474):

HA292-402 :

HA292-474 :

HA306-402 :

HA306-351 :

HA351-474 :

Immune response to VLP-HAs

Immunization with VLP-Has protects mice in vivo

ΔHA stalk (HA292-553):

HA306-474 :

HA403-474 :

LAH (HA420-474):

IgG-specific antibody response of mice vaccinated with VLP-HAs

ΔHA stalk (HA292-553):

HA306-474 :

HA403-474 :

LAH (HA420-474):

HA420-474 (LAH) of different HA subtypes

Ongoing Work

• Immunization with new VLPs in mice

• In vivo challenge-protection assay with H3N2

• Micro-neutralization and ADCC assays