1. Introduction Application of β-glucan
4. Conclusion
High Pressure Homogenizer (HPH, Nano Disperser, ILSHIN AUTOCLAVE)
In the HPH process, the pressure and the number of passes increases makeviscosity and molecular weight decrease without any acid/alkali solution (GreenChemistry).
The FT-IR spectra indicated no obvious modification of chemical structure ofpolymer before and after HPH treatment.
HPH has been shown to be a valid tool to reduce molecular weight of naturalpolymer (polysaccharides structure).
Facile depolymerization process of β-glucan
Using High Pressure Homogenizer
Jun Hee Choa, Taeyoung Kima, Yun Hye Yeona, Hyun Hyo Kima
aDepartment of Research & Development, ILSHINAUTOCLAVE CO. #835 Taplipdong, Yuseonggu, Daejeon 305-510, Korea
2. Experimental
ExperimentalLarge Molecular Weight
5 %(w/v) β-glucan Solution
3. Results & Discussion
Shear Force Cavitation
Entanglement and stretch
Smaller FragmentHigh Molecular Weight
PolymerDepolymerization
Process Method Advantage Disadvantage
Physical
(Radiation)
Ultrasonic • No Waste (Acid/Alkali), No-residues• Long Time Process
• Process Noise
E-beam, γ-Ray • No Waste (Acid/Alkali), No-residues• Dangerous Process
• High Cost
Chemical
Acidic hydrolysis
• High Solubility
• High Efficiency of Depolymerization
• Acid waste water
• Complex Process
Phosphorylation
Sulfation
• Enhancement of Solubility and
Functionality• Low Efficiency
BiologicalEnzymatic hydrolysis
• Selective Decomposition
• High Efficiency of Depolymerization
• High Cost
• Inefficiency
Problems
High Viscosity
&
Large Molecular
Weight
Method of Depolymerization
Plunger
Fluid inlet
Fluid outlet
Hydraulic motive cylinderPlunger seal
Alternatingmotion
High pressure Intensifier
Nozzle Chamber
• Shear Force
• Cavitation
• Impact Force
Inlet
High Pressure Pump
Pressure Gauge
Nozzle Chamber
High Energy
Outlet
Inlet Shear & Cavitation Zone
Impact Zone Outlet
Inlet
Pressure (bar) 500 1000 1500 2000 2500
Fluid velocity (m/sec) 313 442 542 626 700
Inlet
Outlet
Outlet
Impact Zone
Shear & Cavitation Zone
Shear & Cavitation Zone
Impact Zone
Fluid Velocity (Sound Speed: 340 m/s)
Low Molecular Weight
5 %(w/v) β-glucan Solution
- Natural Polymer: β-glucan
- Solvent: D.I Water
- Nano Disperser: NLM 100
Operating Pressure (bar) 200 – 1500
Max. Flow Rate (ml/min) 100
Pump System Motor Driven
Dimension (mm) 300*450*300
Weight (kg) 30
Viscosity(5 %(w/v) β-glucan) Molecular Weight FT-IR Spectrum
• HPH treatment produces significant changes in the viscosity and molecular weight of the β-glucan solution.
• As the pressure and the number of pass increases, the polymer solution shows a significant decrease of viscosity and molecular weight.
• High Depolymerization Effect, Short Process Time
and No Waste Water (Green Chemistry)
- β-glucan structure
Cosmetic Food Drug
TextileDrug Delivery System
Environment
UV-Vis
XRD
Characterization
- Viscometer
- Gel Chromatograph
- FT-IR
- UV-Vis
- XRD
• The intensity characteristics of the
peaks tend to decrease with
increasing numbers of passes under
high pressure homogenization.
• A decrease in crystallinity occurs
due to the destruction of the crystal
structure of chitosan as a result of
the cleavage of the glycosyl bonds
200 250 300 350 400 450
Ab
so
rba
nc
e
Wavelength (nm)
5 Passes
3 Passes
1 Pass
0 Pass
1500 bar
5 10 15 20 25 30 35 40
Inte
ns
ity
(a
rb.u
nit
)
2 Theta (degree)
0 Pass
1 Pass
3 Passes
5 Passes
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