B

A

v3 (FITC) and F-actin (TR)

3

NP-18 NP-9

M21

WT

3-1

moc

kW

T

3-2

3-3

3-4

3-5

3-6

moc

k

moc

k

moc

k

moc

k

3

NP-18 NP-9

M21

WT

3-1

moc

kW

T

3-2

3-3

3-4

3-5

3-6

moc

k

moc

k

moc

k

moc

kNP-93-3 NP-93-4 NP-93-5 NP-93-6

Supplemental figure. NP-9β3 clones: expression of β3 and cell morphology.A. Different clones for NP-9β3 transfected cells were grown in complete medium. After 24 hours, 40 g of total protein extracts were immunoblotted using antibody against β3: β3(AP3). wt: parental, mock: mock-transfected and β3: β3-transfected. B. Cells were seeded on glass coverslips, fixed, permeabilized and stained for: αvβ3 integrin (FITC) and F-actin (TR). All samples were analyzed using confocal microscopy. Bars, 20 m.

Wt 3-2 3-3 3-4 3-5 3-6 mock mock mock mock

Supplemental figure

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46