v 3 (FITC) and F-actin (TR)
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B A v 3 (FITC) and F-actin (TR) NP-9 3 -3 NP-9 3 -4 NP-9 3 -5 NP-9 3 -6 Supplemental figure. NP-9β 3 clones: expression of β 3 and cell morphology. A. Different clones for NP-9β 3 transfected cells were grown in complete medium. After 24 hours, 40 g of total protein extracts were immunoblotted using antibody against β 3 : β 3 (AP3). wt: parental, mock: mock-transfected and β 3 : β 3 -transfected. B. Cells were seeded on glass coverslips, fixed, permeabilized and stained for: α v β 3 integrin (FITC) and F-actin (TR). All samples were analyzed using confocal microscopy. Bars, 20 m. Wt 3-2 3-3 3-4 3-5 3-6 mock mock mock mock Supplemental figure 45
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Supplemental figure. A. Wt b 3-2 b 3-3 b 3-4 b 3-5 b 3-6 mock mock mock mock. B. NP-9 3 -3. NP-9 3 -4. NP-9 3 -5. NP-9 3 -6. v 3 (FITC) and F-actin (TR). Supplemental figure. NP-9 β 3 clones: expression of β 3 and cell morphology. - PowerPoint PPT Presentation
Transcript of v 3 (FITC) and F-actin (TR)
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v3 (FITC) and F-actin (TR)
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Supplemental figure. NP-9β3 clones: expression of β3 and cell morphology.A. Different clones for NP-9β3 transfected cells were grown in complete medium. After 24 hours, 40 g of total protein extracts were immunoblotted using antibody against β3: β3(AP3). wt: parental, mock: mock-transfected and β3: β3-transfected. B. Cells were seeded on glass coverslips, fixed, permeabilized and stained for: αvβ3 integrin (FITC) and F-actin (TR). All samples were analyzed using confocal microscopy. Bars, 20 m.
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Supplemental figure
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