WA90094-Adipocyte Life Cycle - Stemcell Technologies · 2019. 4. 3. · aP2, Glut4, perlilipin,...
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Hypertrophic adipocyte TNF α , ROS, FFA Dying adipocyte perilipin, crown body Mature adipocyte aP2, Glut4, perlilipin, LPL, leptin, resistin Endothelial cell PECAM-1, VE-cadherin, VEGFR2, Tie2 Mural cell SMA, NG2, PDGFR β Adipose stem cell CD34, CD29, Sca-1, CD24, PPAR γ , Zfp423 Transcription factors Signal transduction Pharmacology Lipid storage Hormones PPAR γ C/EBP α C/EBP β , δ KLF5, KLF6 Krox20 SREBP-1c STAT5a Zfp423, Zfp467 TNF α IL-6 TZDs High fat diet TZDs HGF VEGF p21 Cyclin D1 Rb E2Fs Estrogen ADIPOSE DIFFERENTIATION AND OBESIGENIC EXPANSION 1 ADIPOSE STEM CELL PROLIFERATION 2 ADIPOCYTE DIFFERENTIATION 3 ADIPOCYTE TURNOVER 4 ADIPOCYTE OBESIGENIC EXPANSION TZDs Insulin Sulfonylureas Orlistat Sibutramine Phentermine Topiramate CD36 LPL Fatty acid synthase SREBP-1C Perilipin DGAT1 ANGPTL4 BMP FGF PKA Wnt Hh TGF β MAPK PDGFR β TCF7L1 pCREB Pref1 KLF2 GATA2/3 FoxO1 FoxA2 FoxC2 Leptin IGF-1 IGFBP MCSF Insulin IGF1 Estrogen Glucocorticoids Progesterone β -adrenergic Retinoic acid Thyroid TZDs GH VEGF HGF 1 4 3 2 SnapShot: Adipocyte Life Cycle Yuwei Jiang, A-Young Jo, and Jonathan M. Graff Department of Developmental Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA MesenCult™ Your High-performance System for MSC Isolation, Culture, and Differentiation Adipose tissue-derived mesenchymal stromal cells (MSCs), also referred to as adipose tissue-derived stem cells, represent an abundant and accessible source of adult stromal cells with the ability to differentiate along the adipogenic, osteogenic, and chondrogenic lineages. STEMCELL Technologies is committed to providing high-quality, standardized media and reagents for human and mouse MSC isolation, expansion, characterization, and differentiation. Choose from a comprehensive range of MesenCult™ specialty products for both basic and translational research designed to standardize your cell culture system. MesenCult™-ACF Plus Medium Kit (Catalog #05448) Animal component- and serum-free culture kit for isolation and culture of human MSCs. Cells cultured in MesenCult™-ACF Plus expand faster and demonstrate superior differentiation potential compared to cells cultured in serum-based media. Human platelet lysate- and serum-based media for human MSC expansion are also available. MesenCult™ Expansion Kit (Mouse: Catalog #05513) Enrich for and expand mouse MSCs in culture without serial passaging and generate enough cells to perform experiments as early as passage 0. MesenCult™ Adipogenic Differentiation Medium (Human: Catalog #05412) Complete medium specifically formulated for the in vitro differentiation of human bone marrow-, umbilical cord-, and adipose-derived MSCs into adipocytes. It is optimized for cells previously cultured in serum-containing, serum-free, and animal component-free media as well as platelet lysate formulations. MesenCult™-ACF Chondrogenic Differentiation Medium (Catalog #05455) Animal component-free medium for the robust differentiation of human MSCs into chondrocytes. Please visit www.stemcell.com/MesenCult for additional information on all products and resources available to help your MSC research, including cell enrichment and selection kits, antibodies, and a range of primary cell products or contact our knowledgeable technical support team for detailed protocol information at [email protected]. DOCUMENT #900094 | VERSION 1.1.0
Transcript of WA90094-Adipocyte Life Cycle - Stemcell Technologies · 2019. 4. 3. · aP2, Glut4, perlilipin,...
Hypertrophic adipocyteTNFα, ROS, FFA
Dying adipocyteperilipin, crown body
Mature adipocyteaP2, Glut4, perlilipin,LPL, leptin, resistin
Endothelial cellPECAM-1,VE-cadherin,VEGFR2, Tie2
Mural cellSMA, NG2,PDGFRβ
Adipose stem cellCD34, CD29, Sca-1, CD24, PPARγ, Zfp423
Transcription factors
Signal transduction
Pharmacology
Lipid storage
Hormones
PPARγC/EBPαC/EBPβ, δKLF5, KLF6Krox20SREBP-1cSTAT5aZfp423, Zfp467
TNFαIL-6TZDs
High fat dietTZDsHGFVEGF
p21Cyclin D1RbE2FsEstrogen
A D I P O S E D I F F E R E N T I AT I O N A N D O B E S I G E N I C E X PA N S I O N
1 ADIPOSE STEM CELL PROLIFERATION
2 ADIPOCYTE DIFFERENTIATION
3 ADIPOCYTE TURNOVER
4 ADIPOCYTE OBESIGENIC EXPANSION
TZDsInsulinSulfonylureasOrlistat
SibutraminePhentermineTopiramate
CD36LPLFatty acid synthaseSREBP-1CPerilipinDGAT1ANGPTL4
BMPFGFPKAWntHhTGFβMAPKPDGFRβ
TCF7L1pCREBPref1KLF2GATA2/3FoxO1FoxA2FoxC2
LeptinIGF-1IGFBPMCSF
InsulinIGF1EstrogenGlucocorticoidsProgesteroneβ-adrenergicRetinoic acid
ThyroidTZDsGHVEGFHGF
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SnapShot: Adipocyte Life CycleYuwei Jiang, A-Young Jo, and Jonathan M. GraffDepartment of Developmental Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
MesenCult™Your High-performance System for MSCIsolation, Culture, and Differentiation
Adipose tissue-derived mesenchymal stromal cells (MSCs), also referred to as adipose tissue-derived stem cells, represent an abundant and accessible source of adult stromal cells with the ability to differentiate along the adipogenic, osteogenic, and chondrogenic lineages.
STEMCELL Technologies is committed to providing high-quality, standardized media and reagents for human and mouse MSC isolation, expansion, characterization, and differentiation. Choose from a comprehensive range of MesenCult™ specialty products for both basic and translational research designed to standardize your cell culture system.
MesenCult™-ACF Plus Medium Kit(Catalog #05448) Animal component- and serum-free culture kit for isolation and culture of human MSCs. Cells cultured in MesenCult™-ACF Plus expand faster and demonstrate superior differentiation potential compared to cells cultured in serum-based media. Human platelet lysate- and serum-based media for human MSC expansion are also available.
MesenCult™ Expansion Kit(Mouse: Catalog #05513) Enrich for and expand mouse MSCs in culture without serial passaging and generate enough cells to perform experiments as early as passage 0.
MesenCult™ Adipogenic Differentiation Medium (Human: Catalog #05412) Complete medium specifically formulated for the in vitro differentiation of human bone marrow-, umbilical cord-, and adipose-derived MSCs into adipocytes. It is optimized for cells previously cultured in serum-containing, serum-free, and animal component-free media as well as platelet lysate formulations.
MesenCult™-ACF Chondrogenic Differentiation Medium (Catalog #05455) Animal component-free medium for the robust differentiation of human MSCs into chondrocytes.
Please visit www.stemcell.com/MesenCult for additional information on all products and resources available to help your MSC research, including cell enrichment and selection kits, antibodies, and a range of primary cell products or contact our knowledgeable technical support team for detailed protocol information at [email protected].
DOCUMENT #900094 | VERSION 1.1.0
