Test sample chromatograms

Loic Harrault

Method: ArchaeolstescanLH1017FROH TMSReindeer dung

RT: 29.95 - 47.42

30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47

Time (min)

0

2000000

4000000

6000000

8000000

10000000

12000000

14000000

16000000

18000000

20000000

22000000

24000000

26000000

28000000

30000000

32000000

34000000

36000000

38000000

40000000

42000000

44000000

46000000

48000000

50000000

52000000

54000000

56000000

Re

lative

Ab

un

da

nce

36.3234.4731.77

36.13

34.3932.5033.36

37.23

30.68

32.7036.98

30.77

34.7040.06

31.61

33.5538.26

30.90

35.08 41.18

41.8735.46

40.9139.5638.99

46.6737.48 45.8943.70 45.01

43.42

NL:7.26E7

TIC MS LH1017FROH01

Arch

aeol

Copr

osta

nol

Epic

opro

stan

ol?

24-m

ethy

l-5a-

chol

est-

22E-

en-3

b-ol

C28Δ

5,7

or 8

C28Δ

5,7

or 8

24-m

ethy

lcho

lest

a-5,

22-

dien

-3β-

ol

C28O

HCh

oles

tero

lCh

oles

tano

lEp

i-b-c

ampe

stan

ol24

-eth

yl-5

β-ch

oles

ta-2

2-en

-3β-

ol24

-eth

yl-5

β-ch

oles

ta-2

2-en

-3α-

ol

24-e

thyl

-cop

rost

anol

24-e

thyl

-epi

copr

osta

nol

C28Δ

5,7

or 8

Lich

este

rol

Cam

pest

anol

C29

Δ0Sti

gmas

tero

l24

-eth

yl-5

α-ch

oles

ta-2

2-en

-3β-

ol (s

tigm

asta

nol)

24-e

pi-f

ungi

ster

ol o

r Erg

osta

-7-e

n-3b

-ol

C30O

HSi

tost

erol

Sito

stan

ol+

β-am

yrin

Fuco

ster

ol

24-e

thyl

-5α-

chol

est-

24(2

8)Z-

en-3

β-ol

α-am

yrin Sc

hott

enol

Avan

aste

rol

??

C29

or C

30 tr

iterp

ene

alco

hol

Betu

lin d

eriv

ative

Citr

osta

dien

ol +

2 ?

2 ?

Betu

lin?? ?Be

tulin

ic a

cid

Hop

anoi

d

Urs

olic

aci

d*

*

*

**

* *

* = series of unknown compounds with characteristic m/z fragment: 131, 215, 381

LH1017FROH01 #1300 RT: 39.40 AV: 1 SB: 2 39.30, 39.50 NL: 2.62E5T: + c Full ms [ 50.00-650.00]

50 100 150 200 250 300 350 400 450 500 550 600 650

m/z

0

5

10

15

20

25

30

35

40

45

50

55

60

65

70

75

80

85

90

95

100

Re

lativ

e A

bu

nd

an

ce

131.2

215.3

199.373.1

191.295.1

133.2381.6

216.4143.2

382.6282.2 347.4252.3 472.9416.4 488.8 544.2 582.0 602.8

RT: 36.89 - 40.12

37.0 37.5 38.0 38.5 39.0 39.5 40.0

Time (min)

0

50

100

0

50

100

0

50

100

0

50

100

Re

lativ

e A

bu

nd

an

ce 0

50

100

0

50

100

0

50

10039.01

39.4037.2536.98 38.24 39.5937.46 40.0837.80 38.53 38.8438.01

39.4039.01

37.2336.9638.26 39.56

40.0437.51 39.8837.76 38.14 38.44 38.61

39.4037.2537.11

38.26 39.07 39.5637.48 37.80 38.14 39.9038.38 38.99

38.26

37.08 38.6337.9137.5737.19 39.4039.09 40.0238.76 39.73

39.40 39.8837.9137.8036.98 37.29 39.5038.7438.3038.01 39.3039.0338.51

39.40 39.7539.1138.6537.84 39.9438.9137.51 38.2837.23 38.14 38.40 39.54

39.4437.08 37.51 38.26 40.0637.93 38.70 38.9737.70 38.57 39.9639.34

NL:1.03E6

m/z= 130.5-131.5 MS LH1017FROH01

NL:2.47E5

m/z= 214.5-215.5 MS LH1017FROH01

NL:8.86E4

m/z= 198.5-199.5 MS LH1017FROH01

NL:5.43E4

m/z= 380.5-381.5 MS LH1017FROH01

NL:1.62E3

m/z= 581.5-582.5 MS LH1017FROH01

NL:2.11E3

m/z= 543.5-544.5 MS LH1017FROH01

NL:1.74E3

m/z= 601.5-602.5 MS LH1017FROH01