www.kmac.to Surface Plasmon Resonance Analyzer

SPR System Based on Microfluidics Wide Dynamic Range Kinetic Analysis by Detection of Association /Dissociation of Bio-Molecules Measuring of Mass Change below ~10nM.

Θ

Flow Cell

Photo diode DetectorPolarized Light

Coupling Prism

Solution of Protein

Surface refractive index is monitored over time as binding events occur.

Self assembled monolayer50mm Gold Surface

Refr

activ

e In

dex

Time

Inject Analyte

Prism

Ligand

Analyte

Inject Ligand

The SPR signal originates in changes in the refractive index at the surface layer, caused by concentration changed on the sensor chip surface with adsorbed bio-molecules.The SPR is detected by measurement of the intensity of reflected light. From the SPR angle, a sharp decrease or dip in intensity can be measured.Any change in thickness or index of refraction of the material adsorbed onto gold surface results in a shift of the SPR angle. The SPR angle shift is used as a response unit to quantify binding molecules to the sensor surface.

Glass (10mm)

Cr (2nm)Au (50nm)SAM (~3nm)

Substance

5 6

Principles of

SPR System Based on Microfluidics Wide Dynamic Range Kinetic Analysis by Detection of Association /Dissociation of Bio-Molecules Measuring of Mass Change below ~10nM.

Θ

Flow Cell

Photo diode DetectorPolarized Light

Coupling Prism

Solution of Protein

Surface refractive index is monitored over time as binding events occur.

Self assembled monolayer50mm Gold Surface

Refr

activ

e In

dex

Time

Inject Analyte

Prism

Ligand

Analyte

Inject Ligand

The SPR signal originates in changes in the refractive index at the surface layer, caused by concentration changed on the sensor chip surface with adsorbed bio-molecules.The SPR is detected by measurement of the intensity of reflected light. From the SPR angle, a sharp decrease or dip in intensity can be measured.Any change in thickness or index of refraction of the material adsorbed onto gold surface results in a shift of the SPR angle. The SPR angle shift is used as a response unit to quantify binding molecules to the sensor surface.

Glass (10mm)

Cr (2nm)Au (50nm)SAM (~3nm)

Substance

5 6

Principles of

Measurement Mode

Analysis Mode

• Pre-scanning Mode (θ-R plot) : Incident angle vs Reflectance

• Fixed Angle Mode ( T-R plot) : Time vs Reflectance

• Repeated Angle Mode ( T- Rθ plot): Time vs Resonance angle

Pre-scanning mode is the method for measuring reflectance intensity as incident angle of the p-polarized light source and finding minimum reflectance angle.This mode is the angle resolution of 0.01 ~ 0.5 degree and measurement range within 30 ~ 80 degree.

Fixed angle mode is the method for finding the maximum slope of the SPR curve and measuring the reflectivity with real time at this fixed angle . This process is performed in order to rapid monitoring at the minimum 0.2 second interval. As a measuring of change of reflectance with time function, this mode is displayed by “Time vs Reflectance intensity change”.

Repeated angle mode is the method for measuring the change of minimum resonance angle in the constant narrow angle range by repeatedly angle scanning and fitting. As a measuring of resonance angle shift with time function, this mode is displayed by “ Time vs Resonance angle change ”.

The interpretation of the measurement data and of the experimental result using the Simulation Mode, estimation of the experimental result, comparison with the result of theoretical and experimental.

SOFTWARE

Sample Flow Channel

Flow Tube

Flow cell Material

Flow Tube Material

Flow rate

Sample Volume

Pump Method

Sample InjectionMethod

Control Range

Control Method

Temp. Accuracy

Flow

System

Temp.

Control

System

1

0.5mm(dia.)

PEEK + Kalrez O-ring

PEEK

5-100ul/min

Sample Cell : 8.06 ul

Injection Volume : < 100ul (changeable)

1 × Syringe Pump for Buffer and Sample

2-Position Injection Valve(10port)

1st Loop for Sample, 2nd Loop for Air

From 4℃ to 40℃ (5℃/hr)

Peltier Element

± 0.1℃

Sensor

System

Measurement Method

Sensing Point

Light Source

Detector

Incident Angle Range

Incident Angle Change Mechanism

Au Coated Substrate

Outer Dimensions

Weight

Power Supply

Software(Window 98, 2000, XP)

Angle Scan Sensor & Sample Flow

1

Semiconductor Laser(635nm, 2.5mW)

1 × Photodiode

30 - 80 degree

Pulse Motor

18(W)×18(D)×10(H) mm

600(D) × 450(W) × 270(H) mm

45 kg

AC 220V, 60Hz

SPR LAB Ver.2.X

600.00

274.

00

450.00

7 8

SPECIFICATION • DIMENSIONS

UNIT : mm

Measurement Mode

Analysis Mode

• Pre-scanning Mode (θ-R plot) : Incident angle vs Reflectance

• Fixed Angle Mode ( T-R plot) : Time vs Reflectance

• Repeated Angle Mode ( T- Rθ plot): Time vs Resonance angle

Pre-scanning mode is the method for measuring reflectance intensity as incident angle of the p-polarized light source and finding minimum reflectance angle.This mode is the angle resolution of 0.01 ~ 0.5 degree and measurement range within 30 ~ 80 degree.

Fixed angle mode is the method for finding the maximum slope of the SPR curve and measuring the reflectivity with real time at this fixed angle . This process is performed in order to rapid monitoring at the minimum 0.2 second interval. As a measuring of change of reflectance with time function, this mode is displayed by “Time vs Reflectance intensity change”.

Repeated angle mode is the method for measuring the change of minimum resonance angle in the constant narrow angle range by repeatedly angle scanning and fitting. As a measuring of resonance angle shift with time function, this mode is displayed by “ Time vs Resonance angle change ”.

The interpretation of the measurement data and of the experimental result using the Simulation Mode, estimation of the experimental result, comparison with the result of theoretical and experimental.

SOFTWARE

Sample Flow Channel

Flow Tube

Flow cell Material

Flow Tube Material

Flow rate

Sample Volume

Pump Method

Sample InjectionMethod

Control Range

Control Method

Temp. Accuracy

Flow

System

Temp.

Control

System

1

0.5mm(dia.)

PEEK + Kalrez O-ring

PEEK

5-100ul/min

Sample Cell : 8.06 ul

Injection Volume : < 100ul (changeable)

1 × Syringe Pump for Buffer and Sample

2-Position Injection Valve(10port)

1st Loop for Sample, 2nd Loop for Air

From 4℃ to 40℃ (5℃/hr)

Peltier Element

± 0.1℃

Sensor

System

Measurement Method

Sensing Point

Light Source

Detector

Incident Angle Range

Incident Angle Change Mechanism

Au Coated Substrate

Outer Dimensions

Weight

Power Supply

Software(Window 98, 2000, XP)

Angle Scan Sensor & Sample Flow

1

Semiconductor Laser(635nm, 2.5mW)

1 × Photodiode

30 - 80 degree

Pulse Motor

18(W)×18(D)×10(H) mm

600(D) × 450(W) × 270(H) mm

45 kg

AC 220V, 60Hz

SPR LAB Ver.2.X

600.00

274.

00

450.00

7 8

SPECIFICATION • DIMENSIONS

UNIT : mm

SPR Imaging System Based on Array type High Throughput Screening of Target Molecules. Rapid Monitoring of Biochip Ease to Use Powerful image Processing S/W

ΔR

rA

rR

α

Schematic of SPR imaging. A parallel beam is incident on a prism near the SPR resonance angle. The reflected light is detected by a CCD Array. Contrast in SPR image arises from variation in the reflected light intensity ΔR.

With the advance of proteomics and the requirement of analysis of a variety of expressed proteins, rapid and high-throughput detection of the proteins is the hot issue. SPRi detects the specific binding of unlabeled bio-molecules to arrayed capture molecules attached to a modified gold thin film by measuring changes in the local index of refraction upon adsorption. It has been proven to be a valuable tool for investigating molecular interactions without the use of any fluorescent or radiochemical labels.

SPR imaging using fast optical array detectors permits simultaneous measurements across an array of immobilized molecules. SPR imaging provides excellent spatial resolution at the same sensitivity as classical SPR. These features make SPR imaging a promising detection technology for biochips.Also, SPR imaging has applied to bioanalytical chemistry such as label-free investigation of enzyme–substrate interactions, DNA hybridization, antibody–antigen binding and protein–DNA or protein–protein.

CCD arrayLight Source

Parallel Beam

AnalyteMetal LayerPrism

9 10

Principles of

SPR Imaging System Based on Array type High Throughput Screening of Target Molecules. Rapid Monitoring of Biochip Ease to Use Powerful image Processing S/W

ΔR

rA

rR

α

Schematic of SPR imaging. A parallel beam is incident on a prism near the SPR resonance angle. The reflected light is detected by a CCD Array. Contrast in SPR image arises from variation in the reflected light intensity ΔR.

With the advance of proteomics and the requirement of analysis of a variety of expressed proteins, rapid and high-throughput detection of the proteins is the hot issue. SPRi detects the specific binding of unlabeled bio-molecules to arrayed capture molecules attached to a modified gold thin film by measuring changes in the local index of refraction upon adsorption. It has been proven to be a valuable tool for investigating molecular interactions without the use of any fluorescent or radiochemical labels.

SPR imaging using fast optical array detectors permits simultaneous measurements across an array of immobilized molecules. SPR imaging provides excellent spatial resolution at the same sensitivity as classical SPR. These features make SPR imaging a promising detection technology for biochips.Also, SPR imaging has applied to bioanalytical chemistry such as label-free investigation of enzyme–substrate interactions, DNA hybridization, antibody–antigen binding and protein–DNA or protein–protein.

CCD arrayLight Source

Parallel Beam

AnalyteMetal LayerPrism

9 10

Principles of

Spot detection Viewer

Line Profile Viewer

3 Dimension Viewer

This viewer is display detection of target spot and information of spot, which is apply to setting range of detection spot and background . The detection method is automation and manual type.

It is the viewer that display line intensity profiling of X axis or Y- axis image using 2-Dimension image data

It is the viewer that display 3-Dimension image based on intensity of 2-Dimension image data, which is measuring or loading

SOFTWARE

3D Image

Protein Concentration

11 12

Sensor

System

Measurement Method

Sensing Point

Light Source

Detector

Incident Angle Change Mechanism

Au Coated Substrate

Outer Dimensions

Weight

Power Supply

Software(Window 98, 2000, XP)

Prism Material

Intensity Interrogation

Light Emitting Diode (760nm)

Array

BK7, SF10, Fused Silica, BaCD4 or User Modify available

2D Charge Coupled Device (CCD)

Manual type

18(W) x 18(D) x 0.5(H) mm

430(W) x 380(D) x 430(H)

30kg

AC 220V, 60(Hz)

MS window XP

422.

50

433.00 370.19

SPECIFICATION • DIMENSIONS

UNIT : mm

Spot detection Viewer

Line Profile Viewer

3 Dimension Viewer

This viewer is display detection of target spot and information of spot, which is apply to setting range of detection spot and background . The detection method is automation and manual type.

It is the viewer that display line intensity profiling of X axis or Y- axis image using 2-Dimension image data

It is the viewer that display 3-Dimension image based on intensity of 2-Dimension image data, which is measuring or loading

SOFTWARE

3D Image

Protein Concentration

11 12

Sensor

System

Measurement Method

Sensing Point

Light Source

Detector

Incident Angle Change Mechanism

Au Coated Substrate

Outer Dimensions

Weight

Power Supply

Software(Window 98, 2000, XP)

Prism Material

Intensity Interrogation

Light Emitting Diode (760nm)

Array

BK7, SF10, Fused Silica, BaCD4 or User Modify available

2D Charge Coupled Device (CCD)

Manual type

18(W) x 18(D) x 0.5(H) mm

430(W) x 380(D) x 430(H)

30kg

AC 220V, 60(Hz)

MS window XP

422.

50

433.00 370.19

SPECIFICATION • DIMENSIONS

UNIT : mm

The sensor chip is the signal transducer in real time SPR. The chip is a glass slide with a thin layer of gold deposited on one side. Gold is chosen for its combination of chemical inertness and good SPR response. The gold film on the sensor chip is covered with a covalently bound matrix to which bio-molecules may be immobilized using well defined chemistry.

GOLD CHIP

Types of Gold Chip

G1 G2

G3

G5

G6

G1 10㎜×12㎜×0.3㎜

G2 18㎜×18㎜×0.3㎜

G3 25Φ×1.0㎜

G4 Block Prism 90

Model Specipication

G5 25㎜×25㎜×1.0㎜, BK7

G6 Silicone wafer(4")

G7 SF10 Glass

G8 Quartz cell

G9 Slide Glass

G10 Reaction Chamber

G11 Prism reference

G12 Order Made

G4

G9

The sensor chip is the signal transducer in real time SPR. The chip is a glass slide with a thin layer of gold deposited on one side. Gold is chosen for its combination of chemical inertness and good SPR response. The gold film on the sensor chip is covered with a covalently bound matrix to which bio-molecules may be immobilized using well defined chemistry.

GOLD CHIP

Types of Gold Chip

G1 G2

G3

G5

G6

G1 10㎜×12㎜×0.3㎜

G2 18㎜×18㎜×0.3㎜

G3 25Φ×1.0㎜

G4 Block Prism 90

Model Specipication

G5 25㎜×25㎜×1.0㎜, BK7

G6 Silicone wafer(4")

G7 SF10 Glass

G8 Quartz cell

G9 Slide Glass

G10 Reaction Chamber

G11 Prism reference

G12 Order Made

G4

G9

Cleaning & Reaction Holder

The holders can be used SPR sensor chip cleaning and reaction such as SAM formation

Constant Temperature Incubator

13 14

Power Supply

Control Range

Resolution

Display

Interface

AC220V, 60Hz

From 4℃ to 40℃

0.1℃/min

Character LCD

Key pad type

PCH5

PCH1

SCH1

ACCESSORY

Types Prism coupled chip holder(PCH5, PCH1)

Slide chip holder (SCH)

360

136

222UNIT : mm

Address : 104-11 Munji-dong, Yuseong-gu, Daejeon, 305-380, Korea

K-MAC R&D Center

Tel : +82-42-9303-900, +82-42-9303-800Fax : +82-42-8686-867E-mail : spr@kmac.to

Korea Headquarters

554 Yongsan-dong,Yuseong-gu, Daejeon, 305-500 KoreaTel.: +82-42-9303-900Fax: +82-42-9303-979E-mail: sales1@kmac.to

Taiwan Branch

3F., No. 42, Singjhong Rd.,Neihu District, Taipei City 114, Taiwan ROCTel.: +886-2-8791-9296Fax: +886-2-8791-9297

Beijing Office

4-201 2zhuanglou, fuyuandongli 2qu,Beijing jingji jishu kaifaqu, Beijing, ChinaTel.: +86-10-6473-7808 (ext.:878)Fax: +86-10-6472-3808E-mail: Impark@kmac.to

Kunshan Office

41 haobieshu, Zitenghuayuan,Kunshan, Jiangsu 215300, ChinaTel.: +86-512-5717-0842Fax: +86-512-5778-5842

Cleaning & Reaction Holder

The holders can be used SPR sensor chip cleaning and reaction such as SAM formation

Constant Temperature Incubator

13 14

Power Supply

Control Range

Resolution

Display

Interface

AC220V, 60Hz

From 4℃ to 40℃

0.1℃/min

Character LCD

Key pad type

PCH5

PCH1

SCH1

ACCESSORY

Types Prism coupled chip holder(PCH5, PCH1)

Slide chip holder (SCH)

360

136

222UNIT : mm

Address : 104-11 Munji-dong, Yuseong-gu, Daejeon, 305-380, Korea

K-MAC R&D Center

Tel : +82-42-9303-900, +82-42-9303-800Fax : +82-42-8686-867E-mail : spr@kmac.to

Korea Headquarters

554 Yongsan-dong,Yuseong-gu, Daejeon, 305-500 KoreaTel.: +82-42-9303-900Fax: +82-42-9303-979E-mail: sales1@kmac.to

Taiwan Branch

3F., No. 42, Singjhong Rd.,Neihu District, Taipei City 114, Taiwan ROCTel.: +886-2-8791-9296Fax: +886-2-8791-9297

Beijing Office

4-201 2zhuanglou, fuyuandongli 2qu,Beijing jingji jishu kaifaqu, Beijing, ChinaTel.: +86-10-6473-7808 (ext.:878)Fax: +86-10-6472-3808E-mail: Impark@kmac.to

Kunshan Office

41 haobieshu, Zitenghuayuan,Kunshan, Jiangsu 215300, ChinaTel.: +86-512-5717-0842Fax: +86-512-5778-5842

www.kmac.to