Supplementary Figure 1
description
Transcript of Supplementary Figure 1
B
C
A
Supplementary Figure 1
SW620
CoCl2 (h) 0 2 4 8 24
Nur77
β -actin
β -actin
Nur77HCT116
RT-PCR Western Blot
DFX (h) 0 2 4 8 24
SW480 Nur77
β -actin
HT29 Nur77
β -actin
HT29Nur77
18s rRNA
Nur77
β -actin
SW620Nur77
18s rRNA
Nur77
β -actin
HT29
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SW620
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HT29*
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SW620
0 2 4 8 241% O2 (h) 0 2 4 8 241% O2 (hr)
CoCl2 (h) 0 2 4 8 24
DFX (h) 0 2 4 8 24
Supplementary Figure 1. Hypoxic conditions induce the mRNA and protein lev els of Nur77. Colon cancer cells were serum starved for 24 h, and the n (A) incubated at 1% O2 level in a modulator; or treated with the hypoxia mimetic (B) cobalt chloride (CoCl2, 100 mM) or (C) desferrioxamine (DFX, 100 M) for the time indicated. Total RNA or proteins were extracted from cell lysates for subsequent RT-RCR and Western blotting to determine the mRNA and protein expression respectively. Whole-cell lysates were analyzed by immunoblotting. β-actin was used as internal control for these analyses. Immunoblots were quantified by densitometry and the values were normalized with β-actin. All blots shown are representative of three independent experiments. bar, ± SD. *, P < 0.05.