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SAN TA C RUZ BI OTEC HNOL OG Y, INC . SDF-1 (P-159X): … · pre-B cell stimulatory factor, ... use...
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SANTA CRUZ BIOTECHNOLOGY, INC. SDF-1 (P-159X): sc-74271 Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com BACKGROUND The C-X-C or α chemokine family is characterized by a pair of cysteine resi- dues separated by a single amino acid and primarily functions as chemoat- tractants for neutrophils. The C-X-C family includes IL-8, NAP-2, MSGA and stromal cell-derived factor-1, or SDF-1. SDF-1 was originally described as a pre-B cell stimulatory factor, but has now been shown to function as a potent chemoattractant for T cells and monocytes, but not neutrophils. Receptors for the C-X-C family are G protein-coupled, seven-pass transmembrane domain proteins which include IL-8RA, IL-8RB and fusin (also designated LESTR or CXCR-4). Fusin is highly homologous to the IL-8 receptors, sharing 37% se- quence identity at the amino acid level. The IL-8 receptors bind to IL-8, NAP-2 and MSGA, while fusin binds to its cognate ligand, SDF-1. Fusin has been identified as the major co-receptor for T-tropic HIV-1, and SDF-1 has been shown to inhibit HIV-1 infection. Two isforms, SDF-1α and SDF-1β, are the result of alternative splicing of CXCL12, the gene encoding SDF-1. REFERENCES 1. Laterveer, L., et al. 1996. Rapid mobilization of hematopoietic progenitor cells in Rhesus monkeys by a single intravenous injection of interleukin-8. Blood 87: 781-788. 2. Deng, H., et al. 1996. Identification of a major co-receptor for primary isolates of HIV-1. Nature 381: 661-666. 3. Nagasawa, T., et al. 1996. Defects of B cell lymphopoiesis and bone- marrow myelopoiesis in mice lacking the C-X-C chemokine PBSF/SDF-1. Nature 382: 635-638. 4. Bleul, C.C., et al. 1996. The lymphocyte chemoattractant SDF-1 is a ligand for LESTR/fusin and blocks HIV-1 entry. Nature 382: 829-833. CHROMOSOMAL LOCATION Genetic locus: CXCL12 (human) mapping to 10q11.21; Cxcl12 (mouse) mapping to 6 F1. SOURCE SDF-1 (P-159X) is a mouse monoclonal antibody raised against amino acids 20-89 of SDF-1 of human origin. PRODUCT Each vial contains 100 μg IgG 1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and protein stabilizer. STORAGE Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. PROTOCOLS See our web site at www.scbt.com for detailed protocols and support products. APPLICATIONS SDF-1 (P-159X) is recommended for detection of SDF-1α and SDF-1β of mouse, rat and human origin by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500) and immunohistochemistry (including paraffin- embedded sections) (starting dilution 1:50, dilution range 1:50-1:500). Suitable for use as control antibody for SDF-1 siRNA (h): sc-39367, SDF-1 siRNA (m): sc-39368, SDF-1 shRNA Plasmid (h): sc-39367-SH, SDF-1 shRNA Plasmid (m): sc-39368-SH, SDF-1 shRNA (h) Lentiviral Particles: sc-39367-V and SDF-1 shRNA (m) Lentiviral Particles: sc-39368-V. Molecular Weight of SDF-1: 10 kDa. Positive Controls: RAW 264.7 whole cell lysate: sc-2211. RECOMMENDED SUPPORT REAGENTS To ensure optimal results, the following support reagents are recommended: 1) Western Blotting: use m-IgGκ BP-HRP: sc-516102 or m-IgGκ BP-HRP (Cruz Marker): sc-516102-CM (dilution range: 1:1000-1:10000), Cruz Marker™ Molecular Weight Standards: sc-2035, UltraCruz ® Blocking Reagent: sc-516214 and Western Blotting Luminol Reagent: sc-2048. 2) Immunofluo- rescence: use m-IgGκ BP-FITC: sc-516140 or m-IgGκ BP-PE: sc-516141 (dilution range: 1:50-1:200) with UltraCruz ® Mounting Medium: sc-24941 or UltraCruz ® Hard-set Mounting Medium: sc-359850. 3) Immunohisto- chemistry: use m-IgGκ BP-HRP: sc-516102 with DAB, 50X: sc-24982 and Immunohistomount: sc-45086, or Organo/Limonene Mount: sc-45087. SELECT PRODUCT CITATIONS 1. Bhattacharyya, B.J., et al. 2008. The chemokine stromal cell-derived factor-1 regulates GABAergic inputs to neural progenitors in the post- natal dentate gyrus. J. Neurosci. 28: 6720-6730. 2. Katoh, H., et al. 2010. COX-2 and prostaglandin EP3/EP4 signaling regulate the tumor stromal proangiogenic microenvironment via CXCL12-CXCR4 chemokine systems. Am. J. Pathol. 176: 1469-1483. 3. Wilson, N.M., et al. 2011. CXCR4 signaling mediates morphine-induced tactile hyperalgesia. Brain Behav. Immun. 25: 565-573. 4. Schulte, J., et al. 2012. Expression of the E-cadherin repressors Snail, Slug and Zeb1 in urothelial carcinoma of the urinary bladder: relation to stromal fibroblast activation and invasive behaviour of carcinoma cells. Histochem. Cell Biol. 138: 847-860. 5. Liu, X., et al. 2014. Hyperbaric oxygen preconditioning promotes neovascu- larization of transplanted skin flaps in rats. Int. J. Clin. Exp. Pathol. 7: 4734-4744. 6. Roy, L.D., et al. 2014. Systemic neutralization of IL-17A significantly reduces breast cancer associated metastasis in arthritic mice by reducing CXCL12/SDF-1 expression in the metastatic niches. BMC Cancer 14: 225. RESEARCH USE For research use only, not for use in diagnostic procedures.
Transcript of SAN TA C RUZ BI OTEC HNOL OG Y, INC . SDF-1 (P-159X): … · pre-B cell stimulatory factor, ... use...
SANTA CRUZ BIOTECHNOLOGY, INC.
SDF-1 (P-159X): sc-74271
Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com
BACKGROUND
The C-X-C or α chemokine family is characterized by a pair of cysteine resi-dues separated by a single amino acid and primarily functions as chemoat-tractants for neutrophils. The C-X-C family includes IL-8, NAP-2, MSGA andstromal cell-derived factor-1, or SDF-1. SDF-1 was originally described as apre-B cell stimulatory factor, but has now been shown to function as a potentchemoattractant for T cells and monocytes, but not neutrophils. Receptors forthe C-X-C family are G protein-coupled, seven-pass transmembrane domainproteins which include IL-8RA, IL-8RB and fusin (also designated LESTR orCXCR-4). Fusin is highly homologous to the IL-8 receptors, sharing 37% se-quence identity at the amino acid level. The IL-8 receptors bind to IL-8, NAP-2and MSGA, while fusin binds to its cognate ligand, SDF-1. Fusin has beenidentified as the major co-receptor for T-tropic HIV-1, and SDF-1 has beenshown to inhibit HIV-1 infection. Two isforms, SDF-1α and SDF-1β, are theresult of alternative splicing of CXCL12, the gene encoding SDF-1.
REFERENCES
1. Laterveer, L., et al. 1996. Rapid mobilization of hematopoietic progenitorcells in Rhesus monkeys by a single intravenous injection of interleukin-8.Blood 87: 781-788.
2. Deng, H., et al. 1996. Identification of a major co-receptor for primaryisolates of HIV-1. Nature 381: 661-666.
3. Nagasawa, T., et al. 1996. Defects of B cell lymphopoiesis and bone-marrow myelopoiesis in mice lacking the C-X-C chemokine PBSF/SDF-1.Nature 382: 635-638.
4. Bleul, C.C., et al. 1996. The lymphocyte chemoattractant SDF-1 is a ligandfor LESTR/fusin and blocks HIV-1 entry. Nature 382: 829-833.
CHROMOSOMAL LOCATION
Genetic locus: CXCL12 (human) mapping to 10q11.21; Cxcl12 (mouse) mappingto 6 F1.
SOURCE
SDF-1 (P-159X) is a mouse monoclonal antibody raised against amino acids20-89 of SDF-1 of human origin.
PRODUCT
Each vial contains 100 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1%sodium azide and protein stabilizer.
STORAGE
Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date ofshipment. Non-hazardous. No MSDS required.
PROTOCOLS
See our web site at www.scbt.com for detailed protocols and supportproducts.
APPLICATIONS
SDF-1 (P-159X) is recommended for detection of SDF-1α and SDF-1β ofmouse, rat and human origin by Western Blotting (starting dilution 1:200,dilution range 1:100-1:1000), immunofluorescence (starting dilution 1:50,dilution range 1:50-1:500) and immunohistochemistry (including paraffin-embedded sections) (starting dilution 1:50, dilution range 1:50-1:500).
Suitable for use as control antibody for SDF-1 siRNA (h): sc-39367, SDF-1siRNA (m): sc-39368, SDF-1 shRNA Plasmid (h): sc-39367-SH, SDF-1 shRNAPlasmid (m): sc-39368-SH, SDF-1 shRNA (h) Lentiviral Particles: sc-39367-Vand SDF-1 shRNA (m) Lentiviral Particles: sc-39368-V.
Molecular Weight of SDF-1: 10 kDa.
Positive Controls: RAW 264.7 whole cell lysate: sc-2211.
RECOMMENDED SUPPORT REAGENTS
To ensure optimal results, the following support reagents are recommended:1) Western Blotting: use m-IgGκ BP-HRP: sc-516102 or m-IgGκ BP-HRP (CruzMarker): sc-516102-CM (dilution range: 1:1000-1:10000), Cruz Marker™Molecular Weight Standards: sc-2035, UltraCruz® Blocking Reagent:sc-516214 and Western Blotting Luminol Reagent: sc-2048. 2) Immunofluo-rescence: use m-IgGκ BP-FITC: sc-516140 or m-IgGκ BP-PE: sc-516141(dilution range: 1:50-1:200) with UltraCruz® Mounting Medium: sc-24941or UltraCruz® Hard-set Mounting Medium: sc-359850. 3) Immunohisto-chemistry: use m-IgGκ BP-HRP: sc-516102 with DAB, 50X: sc-24982 andImmunohistomount: sc-45086, or Organo/Limonene Mount: sc-45087.
SELECT PRODUCT CITATIONS
1. Bhattacharyya, B.J., et al. 2008. The chemokine stromal cell-derivedfactor-1 regulates GABAergic inputs to neural progenitors in the post-natal dentate gyrus. J. Neurosci. 28: 6720-6730.
2. Katoh, H., et al. 2010. COX-2 and prostaglandin EP3/EP4 signaling regulatethe tumor stromal proangiogenic microenvironment via CXCL12-CXCR4chemokine systems. Am. J. Pathol. 176: 1469-1483.
3. Wilson, N.M., et al. 2011. CXCR4 signaling mediates morphine-inducedtactile hyperalgesia. Brain Behav. Immun. 25: 565-573.
4. Schulte, J., et al. 2012. Expression of the E-cadherin repressors Snail, Slugand Zeb1 in urothelial carcinoma of the urinary bladder: relation to stromalfibroblast activation and invasive behaviour of carcinoma cells. Histochem.Cell Biol. 138: 847-860.
5. Liu, X., et al. 2014. Hyperbaric oxygen preconditioning promotes neovascu-larization of transplanted skin flaps in rats. Int. J. Clin. Exp. Pathol. 7:4734-4744.
6. Roy, L.D., et al. 2014. Systemic neutralization of IL-17A significantlyreduces breast cancer associated metastasis in arthritic mice by reducingCXCL12/SDF-1 expression in the metastatic niches. BMC Cancer 14: 225.
RESEARCH USE
For research use only, not for use in diagnostic procedures.
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