GSK-3β-mediated fatty acid synthesis enhances epithelial ...
SAN TA C RUZ BI OTEC HNOL OG Y, INC . 3β-HSD (A-1): sc … · No MSDS required. APPLICATIONS...
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SANTA CRUZ BIOTECHNOLOGY, INC. 3β-HSD (A-1): sc-515120 Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com BACKGROUND 3β-hydroxysteroid dehydrogenase (3β-HSD), also known as HSD3B1 or HSDB3, is a bifunctional enzyme that plays a crucial role in the synthesis of all classes of hormonal steroids. Two human 3β-HSD proteins, designated type I (3β-HSD) and type II (3β-HSD2), are expressed by different genes and function in different areas of the body. Localized to the membrane of the endoplasmic reticulum (ER) and expressed in skin and placenta, 3β-HSD is the type I protein that catalyzes the oxidative conversion of δ5-ene-3-β- hydroxy steroid, as well as the conversion of various ketosteroids. Defects in the gene encoding 3β-HSD are associated with classic salt wasting, genital ambiguity, hypogonadism, Insulin-resistant polycystic ovary syndrome (PCOS) and an increased susceptibility to prostate cancer. Additionally, congenital deficiency of 3β-HSD activity results in a severe depletion of steroid formation which can be lethal in young children. REFERENCES 1. Thomas, J.L., et al. 2002. Structure/function relationships responsible for the kinetic differences between human type 1 and type 2 3β-hydroxysteroid dehydrogenase and for the catalysis of the type 1 activity. J. Biol. Chem. 277: 42795-42801. 2. Thomas, J.L., et al. 2003. Structure/function relationships responsible for coenzyme specificity and the isomerase activity of human type 1 3β-hydroxysteroid dehydrogenase/isomerase. J. Biol. Chem. 278: 35483-35490. CHROMOSOMAL LOCATION Genetic locus: HSD3B1/HSD3B2 (human) mapping to 1p12; Hsd3b1/Hsd3b2 (mouse) mapping to 3 F2.2. SOURCE 3β-HSD (A-1) is a mouse monoclonal antibody specific for an epitope mapping between amino acids 152-178 within an internal region of 3β-HSD of human origin. PRODUCT Each vial contains 200 μg IgG 3 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. 3β-HSD (A-1) is available conjugated to agarose (sc-515120 AC), 500 μg/ 0.25 ml agarose in 1 ml, for IP; to HRP (sc-515120 HRP), 200 μg/ml, for WB, IHC(P) and ELISA; and to either phycoerythrin (sc-515120 PE), fluorescein (sc-515120 FITC), Alexa Fluor ® 488 (sc-515120 AF488) or Alexa Fluor ® 647 (sc-515120 AF647), 200 μg/ml, for IF, IHC(P) and FCM. Blocking peptide available for competition studies, sc-515120 P, (100 μg peptide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% stabilizer protein). Alexa Fluor ® is a trademark of Molecular Probes, Inc., Oregon, USA STORAGE Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. APPLICATIONS 3β-HSD (A-1) is recommended for detection of 3β-HSD and 3β-HSD2 of mouse, rat and human origin by Western Blotting (starting dilution 1:100, dilution range 1:100-1:1000), immunoprecipitation [1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500), immunohistochemistry (including paraffin- embedded sections) (starting dilution 1:50, dilution range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000). Molecular Weight of 3β-HSD: 42 kDa. Positive Controls: rat adrenal gland extract: sc-364802. RECOMMENDED SUPPORT REAGENTS To ensure optimal results, the following support reagents are recommended: 1) Western Blotting: use m-IgGκ BP-HRP: sc-516102 or m-IgGκ BP-HRP (Cruz Marker): sc-516102-CM (dilution range: 1:1000-1:10000), Cruz Marker™ Molecular Weight Standards: sc-2035, UltraCruz ® Blocking Reagent: sc-516214 and Western Blotting Luminol Reagent: sc-2048. 2) Immunopre- cipitation: use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/2.0 ml). 3) Immunofluorescence: use m-IgGκ BP-FITC: sc-516140 or m-IgGκ BP-PE: sc-516141 (dilution range: 1:50-1:200) with UltraCruz ® Mounting Medium: sc-24941 or UltraCruz ® Hard-set Mounting Medium: sc-359850. 4) Immuno- histochemistry: use m-IgGκ BP-HRP: sc-516102 with DAB, 50X: sc-24982 and Immunohistomount: sc-45086, or Organo/Limonene Mount: sc-45087. DATA SELECT PRODUCT CITATIONS 1. Duan, T., et al. 2016. Role of peroxiredoxin 2 in H 2 O 2 -induced oxidative stress of primary Leydig cells. Mol. Med. Rep. 13: 4807-4813. 2. Wang, W., et al. 2017. Continuous soy isoflavones exposure from weaning to maturity induces downregulation of ovarian steroidogenic factor 1 gene expression and corresponding changes in DNA methylation pattern. Toxicol. Lett. 281: 175-183. RESEARCH USE For research use only, not for use in diagnostic procedures. 3β-HSD (A-1): sc-515120. Near-infrared western blot analysis of 3β-HSD expression in rat adrenal gland tissue extract. Blocked with UltraCruz ® Blocking Reagent: sc-516214. Detection reagent used: m-IgGκ BP-CFL 680: sc-516180. 51K– 35K– < 3β-HSD 3β-HSD (A-1): sc-515120. Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing cytoplasmic staining of trophoblastic cells (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue show- ing cytoplasmic staining of glandular cells (B). B A
Transcript of SAN TA C RUZ BI OTEC HNOL OG Y, INC . 3β-HSD (A-1): sc … · No MSDS required. APPLICATIONS...
SANTA CRUZ BIOTECHNOLOGY, INC.
3β-HSD (A-1): sc-515120
Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com
BACKGROUND
3β-hydroxysteroid dehydrogenase (3β-HSD), also known as HSD3B1 orHSDB3, is a bifunctional enzyme that plays a crucial role in the synthesis ofall classes of hormonal steroids. Two human 3β-HSD proteins, designatedtype I (3β-HSD) and type II (3β-HSD2), are expressed by different genes andfunction in different areas of the body. Localized to the membrane of theendoplasmic reticulum (ER) and expressed in skin and placenta, 3β-HSD isthe type I protein that catalyzes the oxidative conversion of δ5-ene-3-β-hydroxy steroid, as well as the conversion of various ketosteroids. Defects inthe gene encoding 3β-HSD are associated with classic salt wasting, genitalambiguity, hypogonadism, Insulin-resistant polycystic ovary syndrome (PCOS)and an increased susceptibility to prostate cancer. Additionally, congenitaldeficiency of 3β-HSD activity results in a severe depletion of steroid formationwhich can be lethal in young children.
REFERENCES
1. Thomas, J.L., et al. 2002. Structure/function relationships responsible forthe kinetic differences between human type 1 and type 2 3β-hydroxysteroiddehydrogenase and for the catalysis of the type 1 activity. J. Biol. Chem.277: 42795-42801.
2. Thomas, J.L., et al. 2003. Structure/function relationships responsiblefor coenzyme specificity and the isomerase activity of human type 13β-hydroxysteroid dehydrogenase/isomerase. J. Biol. Chem. 278:35483-35490.
CHROMOSOMAL LOCATION
Genetic locus: HSD3B1/HSD3B2 (human) mapping to 1p12; Hsd3b1/Hsd3b2(mouse) mapping to 3 F2.2.
SOURCE
3β-HSD (A-1) is a mouse monoclonal antibody specific for an epitope mappingbetween amino acids 152-178 within an internal region of 3β-HSD of humanorigin.
PRODUCT
Each vial contains 200 µg IgG3 kappa light chain in 1.0 ml of PBS with < 0.1%sodium azide and 0.1% gelatin.
3β-HSD (A-1) is available conjugated to agarose (sc-515120 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; to HRP (sc-515120 HRP), 200 µg/ml, for WB,IHC(P) and ELISA; and to either phycoerythrin (sc-515120 PE), fluorescein(sc-515120 FITC), Alexa Fluor® 488 (sc-515120 AF488) or Alexa Fluor® 647(sc-515120 AF647), 200 µg/ml, for IF, IHC(P) and FCM.
Blocking peptide available for competition studies, sc-515120 P, (100 µgpeptide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% stabilizerprotein).
Alexa Fluor® is a trademark of Molecular Probes, Inc., Oregon, USA
STORAGE
Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date ofshipment. Non-hazardous. No MSDS required.
APPLICATIONS
3β-HSD (A-1) is recommended for detection of 3β-HSD and 3β-HSD2 ofmouse, rat and human origin by Western Blotting (starting dilution 1:100,dilution range 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µgof total protein (1 ml of cell lysate)], immunofluorescence (starting dilution1:50, dilution range 1:50-1:500), immunohistochemistry (including paraffin-embedded sections) (starting dilution 1:50, dilution range 1:50-1:500) andsolid phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000).
Molecular Weight of 3β-HSD: 42 kDa.
Positive Controls: rat adrenal gland extract: sc-364802.
RECOMMENDED SUPPORT REAGENTS
To ensure optimal results, the following support reagents are recommended:1) Western Blotting: use m-IgGκ BP-HRP: sc-516102 or m-IgGκ BP-HRP (CruzMarker): sc-516102-CM (dilution range: 1:1000-1:10000), Cruz Marker™Molecular Weight Standards: sc-2035, UltraCruz® Blocking Reagent:sc-516214 and Western Blotting Luminol Reagent: sc-2048. 2) Immunopre-cipitation: use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/2.0 ml).3) Immunofluorescence: use m-IgGκ BP-FITC: sc-516140 or m-IgGκ BP-PE:sc-516141 (dilution range: 1:50-1:200) with UltraCruz® Mounting Medium:sc-24941 or UltraCruz® Hard-set Mounting Medium: sc-359850. 4) Immuno-histochemistry: use m-IgGκ BP-HRP: sc-516102 with DAB, 50X: sc-24982and Immunohistomount: sc-45086, or Organo/Limonene Mount: sc-45087.
DATA
SELECT PRODUCT CITATIONS
1. Duan, T., et al. 2016. Role of peroxiredoxin 2 in H2O2-induced oxidativestress of primary Leydig cells. Mol. Med. Rep. 13: 4807-4813.
2. Wang, W., et al. 2017. Continuous soy isoflavones exposure from weaningto maturity induces downregulation of ovarian steroidogenic factor 1gene expression and corresponding changes in DNA methylation pattern.Toxicol. Lett. 281: 175-183.
RESEARCH USE
For research use only, not for use in diagnostic procedures.
3β-HSD (A-1): sc-515120. Near-infrared western blotanalysis of 3β-HSD expression in rat adrenal glandtissue extract. Blocked with UltraCruz® BlockingReagent: sc-516214. Detection reagent used: m-IgGκBP-CFL 680: sc-516180.
51 K –
35 K –
< 3β-HSD
3β-HSD (A-1): sc-515120. Immunoperoxidase stainingof formalin fixed, paraffin-embedded human placentatissue showing cytoplasmic staining of trophoblasticcells (A). Immunoperoxidase staining of formalin fixed,paraffin-embedded human adrenal gland tissue show-ing cytoplasmic staining of glandular cells (B).
BA
