CCP1Polyclonal ANTIBODYCatalog Number:14067-1-AP Featured Product 8 Publications

For Research Use Only

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Basic Information Catalog Number:14067-1-APSize:26 μg/150 μlSource:RabbitIsotype:IgGPurif ication Method:Antigen aff inity purif icationImmunogen Catalog Number:AG5122

GenBank Accession Number:BC060815GeneID (NCBI):23287Full Name:ATP/GTP binding protein 1Calculated MW:138 kDaObserved MW:138 kDa

Recommended Dilutions:WB 1:500-1:3000 IP 0.5-4.0 ug for IP and 1:500-1:2000 for WBIHC 1:50-1:500 IF 1:10-1:100

Applications Tested Applications:IF, IHC, IP, WB, ELISACited Applications:IF, IHC, WBSpecies Specif icity:human, mouseCited Species:human, mouse

Positive Controls:WB : mouse brain tissue; A549 cells, mouse testistissueIP : mouse testis tissue;IHC : human testis tissue; human heart tissueIF : A549 cells;

Background Information AGTPBP1, also known as CCP1 (cytosolic carboxy-peptidase 1), or Nna1, belongs to the peptidase M14 family. AGTPBP1 is a zinc carboxypeptidase thatcontains nuclear localization signals and an ATP/GTP-binding motif f . Thus, it may contribute to nuclear signaling events in dif ferentiating and regeneratingneurons. It serves important functions in the nervous system, and AGTPBP1 mutations may cause Purkinje cell degeneration (pcd) phenotypes.

Notable Publications Author Pubmed ID Journal Application

Albert Herms 26013497 Nat Commun WB

Shouta Kitano 26106267 J Cent Nerv Syst Dis WB, IHC, IF

Buqing Ye 29593216 Nat Commun WB

Storage Storage:Store at -20ºC. Stable for one year af ter shipment.Storage Buffer:PBS with 0.02% sodium azide and 50% glycerol pH 7.3.Aliquoting is unnecessary for -20ºC storage

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This product is exclusively available underProteintech Group brand and is not available topurchase from any other manufacturer.

Selected Validation Data

mouse brain tissue were subjected to SDS PAGEfollowed by western blot with 14067-1-AP( CCP1Antibody) at dilution of 1:1500 incubated at roomtemperature for 1.5 hours

IP Result of anti-CCP1 (IP:14067-1-AP, 3ug;Detection:14067-1-AP 1:1000) with mouse testistissue lysate 10000ug.

Immunohistochemistry of paraff in-embeddedhuman testis tissue slide using 14067-1-AP(CCP1 Antibody) at dilution of 1:200 (under 10xlens).

Immunofluorescent analysis of ( -20 Ethanol )f ixed A549 cells using 14067-1-AP(CCP1antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Aff iniPure Goat Anti-Rabbit IgG(H+L)