For Research Use Only SDHB Polyclonal ANTIBODY Catalog Number:10620-1-AP Featured Product 9...

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Transcript of For Research Use Only SDHB Polyclonal ANTIBODY Catalog Number:10620-1-AP Featured Product 9...

  • SDHB Polyclonal ANTIBODY Catalog Number:10620-1-AP Featured Product 10 Publications

    For Research Use Only

    www.ptglab.com

    Basic Information Catalog Number: 10620-1-AP

    Size: 150UL , Concentration: 133 μg/ml by Bradford method using BSA as the standard;

    Source: Rabbit

    Isotype: IgG

    Immunogen Catalog Number: AG0968

    GenBank Accession Number: BC007840

    GeneID (NCBI): 6390

    Full Name: succinate dehydrogenase complex, subunit B, iron sulfur (Ip)

    Calculated MW: 32 kDa

    Observed MW: 28-32 kDa

    Purification Method: Antigen affinity purification

    Recommended Dilutions: WB 1:5000-1:50000 IP 0.5-4.0 ug for IP and 1:500-1:1000 for WB IHC 1:100-1:400 IF 1:50-1:500

    Applications Tested Applications: IF, IHC, IP, WB, ELISA

    Cited Applications: IF, IHC, WB

    Species Specificity: human, mouse, rat

    Cited Species: human, mouse, rat

    Note-IHC: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

    Positive Controls:

    WB : mouse heart tissue, mouse liver tissue, HepG2 cells, HEK-293 cells, rat heart tissue

    IP : HEK-293 cells,

    IHC : human heart tissue, human renal cell carcinoma tissue, human kidney tissue, mouse kidney tissue

    IF : HepG2 cells,

    Background Information SDHB(Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial) is also named as SDH, SDH1 and belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. The SDHD, SDHB, and SDHC genes encode subunits of mitochondrial complex II (succinate dehydrogenase). Mitochondrial complex II is a heterotetrameric complex involved in the aerobic electron transport chain and catalyses the oxidation of succinate to fumarate (Krebs cycle) with transport of electrons to the ubiquinone pool. SDHA and SDHB are the hydrophilic catalytic part of the complex and are highly conserved(J Med Genet 2004;41:e99). Defects in SDHB are a cause of susceptibility to pheochromocytoma (PCC), paragangliomas type 4 (PGL4), paraganglioma and gastric stromal sarcoma (PGGSS) and Cowden-like syndrome (CWDLS).

    Notable Publications Author Pubmed ID Journal Application Yiqiang Huang 30352407 Endocr Connect IHC

    Yi Zeng 31236191 Oxid Med Cell Longev WB

    Hao Wu 30898010 Autophagy WB

    Storage Storage: Store at -20°C. Stable for one year after shipment. Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

    Aliquoting is unnecessary for -20ºC storage

    T: 1 (888) 4PTGLAB (1-888-478-4522) (toll free in USA), or 1(312) 455-8498 (outside USA)

    E: proteintech@ptglab.com W: ptglab.com

    For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech Group brand and is not available to purchase from any other manufacturer.

  • Selected Validation Data

    Various lysates were subjected to SDS PAGE followed by western blot with 10620-1-AP (SDHB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

    Immunohistochemical analysis of paraffin- embedded human heart tissue slide using 10620-1- AP (SDHB Antibody) at dilution of 1:200 (under 10x lens).

    Immunohistochemical analysis of paraffin- embedded human heart tissue slide using 10620-1- AP (SDHB Antibody) at dilution of 1:200 (under 40x lens).

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10620-1-AP (SDHB antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

    IP Result of anti-SDHB (IP:10620-1-AP, 4ug; Detection:10620-1-AP 1:500) with HEK-293 cells lysate 2800ug.