For Research Use Only SDHB Polyclonal ANTIBODY … · Catalog Number:10620-1-AP Featured Product 9...
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SDHB Polyclonal ANTIBODY Catalog Number: 10620-1-AP Featured Product 10 Publications For Research Use Only www.ptglab.com Basic Information Catalog Number: 10620-1-AP Size: 150UL , Concentration: 133 μg/ml by Bradford method using BSA as the standard; Source: Rabbit Isotype: IgG Immunogen Catalog Number: AG0968 GenBank Accession Number: BC007840 GeneID (NCBI): 6390 Full Name: succinate dehydrogenase complex, subunit B, iron sulfur (Ip) Calculated MW: 32 kDa Observed MW: 28-32 kDa Purification Method: Antigen affinity purification Recommended Dilutions: WB 1:5000-1:50000 IP 0.5-4.0 ug for IP and 1:500-1:1000 for WB IHC 1:100-1:400 IF 1:50-1:500 Applications Tested Applications: IF, IHC, IP, WB, ELISA Cited Applications: IF, IHC, WB Species Specificity: human, mouse, rat Cited Species: human, mouse, rat Note-IHC: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 Positive Controls: WB : mouse heart tissue, mouse liver tissue, HepG2 cells, HEK-293 cells, rat heart tissue IP : HEK-293 cells, IHC : human heart tissue, human renal cell carcinoma tissue, human kidney tissue, mouse kidney tissue IF : HepG2 cells, Background Information SDHB(Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial) is also named as SDH, SDH1 and belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. The SDHD, SDHB, and SDHC genes encode subunits of mitochondrial complex II (succinate dehydrogenase). Mitochondrial complex II is a heterotetrameric complex involved in the aerobic electron transport chain and catalyses the oxidation of succinate to fumarate (Krebs cycle) with transport of electrons to the ubiquinone pool. SDHA and SDHB are the hydrophilic catalytic part of the complex and are highly conserved(J Med Genet 2004;41:e99). Defects in SDHB are a cause of susceptibility to pheochromocytoma (PCC), paragangliomas type 4 (PGL4), paraganglioma and gastric stromal sarcoma (PGGSS) and Cowden-like syndrome (CWDLS). Notable Publications Author Pubmed ID Journal Application Yiqiang Huang 30352407 Endocr Connect IHC Yi Zeng 31236191 Oxid Med Cell Longev WB Hao Wu 30898010 Autophagy WB Storage Storage: Store at -20°C. Stable for one year after shipment. Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20 º C storage T: 1 (888) 4PTGLAB (1-888-478-4522) (toll free in USA), or 1(312) 455-8498 (outside USA) E: [email protected] W: ptglab.com For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech Group brand and is not available to purchase from any other manufacturer.
Transcript of For Research Use Only SDHB Polyclonal ANTIBODY … · Catalog Number:10620-1-AP Featured Product 9...
SDHB Polyclonal ANTIBODYCatalog Number:10620-1-AP Featured Product 10 Publications
For Research Use Only
www.ptglab.com
Basic Information Catalog Number:10620-1-AP
Size:150UL , Concentration: 133 μg/ml byBradford method using BSA as thestandard;
Source:Rabbit
Isotype:IgG
Immunogen Catalog Number:AG0968
GenBank Accession Number:BC007840
GeneID (NCBI):6390
Full Name:succinate dehydrogenase complex,subunit B, iron sulfur (Ip)
Calculated MW:32 kDa
Observed MW:28-32 kDa
Purification Method:Antigen affinity purification
Recommended Dilutions:WB 1:5000-1:50000 IP 0.5-4.0 ug for IP and 1:500-1:1000for WBIHC 1:100-1:400 IF 1:50-1:500
Applications Tested Applications:IF, IHC, IP, WB, ELISA
Cited Applications:IF, IHC, WB
Species Specificity:human, mouse, rat
Cited Species:human, mouse, rat
Note-IHC: suggested antigen retrieval withTE buffer pH 9.0; (*) Alternatively, antigenretrieval may be performed with citratebuffer pH 6.0
Positive Controls:
WB : mouse heart tissue, mouse liver tissue, HepG2cells, HEK-293 cells, rat heart tissue
IP : HEK-293 cells,
IHC : human heart tissue, human renal cell carcinomatissue, human kidney tissue, mouse kidney tissue
IF : HepG2 cells,
Background Information SDHB(Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial) is also named as SDH, SDH1 andbelongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. The SDHD, SDHB, and SDHCgenes encode subunits of mitochondrial complex II (succinate dehydrogenase). Mitochondrial complex II is aheterotetrameric complex involved in the aerobic electron transport chain and catalyses the oxidation of succinateto fumarate (Krebs cycle) with transport of electrons to the ubiquinone pool. SDHA and SDHB are the hydrophiliccatalytic part of the complex and are highly conserved(J Med Genet 2004;41:e99). Defects in SDHB are a cause ofsusceptibility to pheochromocytoma (PCC), paragangliomas type 4 (PGL4), paraganglioma and gastric stromalsarcoma (PGGSS) and Cowden-like syndrome (CWDLS).
Notable Publications Author Pubmed ID Journal Application
Yiqiang Huang 30352407 Endocr Connect IHC
Yi Zeng 31236191 Oxid Med Cell Longev WB
Hao Wu 30898010 Autophagy WB
Storage Storage:Store at -20°C. Stable for one year after shipment.Storage Buffer:PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Aliquoting is unnecessary for -20ºC storage
T: 1 (888) 4PTGLAB (1-888-478-4522) (toll freein USA), or 1(312) 455-8498 (outside USA)
E: [email protected] W: ptglab.com
For technical support and original validation data for this product please contact: This product is exclusively available under ProteintechGroup brand and is not available to purchase from anyother manufacturer.
Selected Validation Data
Various lysates were subjected to SDS PAGEfollowed by western blot with 10620-1-AP (SDHBantibody) at dilution of 1:20000 incubated at roomtemperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10620-1-AP (SDHB Antibody) at dilution of 1:200 (under 10xlens).
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10620-1-AP (SDHB Antibody) at dilution of 1:200 (under 40xlens).
Immunofluorescent analysis of (4% PFA) fixedHepG2 cells using 10620-1-AP (SDHB antibody) atdilution of 1:100 and CoraLite488-ConjugatedAffiniPure Goat Anti-Rabbit IgG(H+L).
IP Result of anti-SDHB (IP:10620-1-AP, 4ug;Detection:10620-1-AP 1:500) with HEK-293 cellslysate 2800ug.
