PiNK Plus Prestained Protein Ladder PiNK Plus Prestained Protein … · 2020. 8. 14. · GeneDireX,...
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GeneDireX, Inc. www.genedirex.com [email protected] PiNK Plus Prestained Protein Ladder Cat. No.: SP005-0600 Size: 300 μl x 2 vials Cat. No.: SP005-0025 Size: 25 μl Description The PiNK Plus Prestained Protein Ladder contains 11 proteins that resolve into sharp, tight bands in the range of 10-175 kilodalton (kDa). The PiNK Plus Prestained Protein Ladder allows you to monitor molecular weight separation during on SDS- polyacrylamide gel electrophoresis (SDS-PAGE), estimate molecular weights of proteins of interest, and evaluate western transfer efficiency. Features Broad range: 10-175 kDa (Tris-glycine-SDS running buffer) Ready-to-use: supplied in a loading buffer for direct loading on gels Easy to identify: includes the ~10, ~40 and ~90 kDa reference bands coupled with an blue dyes Sharp bands Applications Monitoring of protein migration during SDS-PAGE gel electrophoresis. Monitoring of protein transfer onto membranes during Western blots. Sizing of proteins on SDS-PAGE gels and Western blots. Storage Buffer Approximately 0.2~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 1 mM Dithiothreitol, 4.8 M Urea, and 12 % (v/v) Glycerol. Quality Control The quality of the PiNK Plus Prestained Protein Ladder is tested on a lot-to-lot basis to ensure consistent product quality. Storage Stable for up to 2 weeks at 25°C. Stable for up to 3 months at 4°C. For long term storage, store at -20°C. PiNK Plus Prestained Protein Ladder Protocol 1. Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil. 2. Mix thoroughly to ensure the solution is homogeneous. 3. Load the following volumes of the ladder on SDS-PAGE gel: 5 μl per well for mini-gels, 2.5 μl per well for blots 10 μl per well for large gels, 5 μl per well for blots Apply more for thicker (> 1.5 mm) or larger gel Guide for Molecular Weight Estimation (kDa) Migration patterns of PiNK Plus Prestained Protein Ladder in different electrophoresis conditions are listed below: kDa ~90 ~70 ~175 ~130 ~60 ~50 ~40 ~10 ~30 ~20 ~15 kDa ~80 ~61 ~165 ~120 ~52 ~42 ~32 ~10 ~24 ~17 ~13 kDa ~82 ~62 ~165 ~125 ~55 ~45 ~34 ~7 ~26 ~16 ~12 Tris-Glycine 15% Bis-Tris 4~12% MES buffer Bis-Tris 4~12% MOPS buffer
Transcript of PiNK Plus Prestained Protein Ladder PiNK Plus Prestained Protein … · 2020. 8. 14. · GeneDireX,...
GeneDireX, [email protected]
PiNK Plus Prestained Protein LadderCat. No.: SP005-0600 Size: 300 μl x 2 vialsCat. No.: SP005-0025 Size: 25 μl
DescriptionThe PiNK Plus Prestained Protein Ladder contains 11 proteins that resolve into sharp, tight bands in the range of 10-175 kilodalton (kDa). The PiNK Plus Prestained Protein Ladder allows you to monitor molecular weight separation during on SDS-polyacrylamide gel electrophoresis (SDS-PAGE), estimate molecular weights of proteins of interest, and evaluate western transfer efficiency.
Features Broad range: 10-175 kDa (Tris-glycine-SDS running buffer) Ready-to-use: supplied in a loading buffer for direct loading on gels Easy to identify: includes the ~10, ~40 and ~90 kDa reference bands coupled with an blue dyes Sharp bands
Applications Monitoring of protein migration during SDS-PAGE gel electrophoresis. Monitoring of protein transfer onto membranes during Western blots. Sizing of proteins on SDS-PAGE gels and Western blots.
Storage BufferApproximately 0.2~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 1 mM Dithiothreitol, 4.8 M Urea, and 12 % (v/v) Glycerol.
Quality ControlThe quality of the PiNK Plus Prestained Protein Ladder is tested on a lot-to-lot basis to ensure consistent product quality.
StorageStable for up to 2 weeks at 25°C.Stable for up to 3 months at 4°C.For long term storage, store at -20°C.
PiNK Plus Prestained Protein Ladder Protocol1. Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil.2. Mix thoroughly to ensure the solution is homogeneous.3. Load the following volumes of the ladder on SDS-PAGE gel: 5 μl per well for mini-gels, 2.5 μl per well for blots 10 μl per well for large gels, 5 μl per well for blots Apply more for thicker (> 1.5 mm) or larger gel Guide for Molecular Weight Estimation (kDa)Migration patterns of PiNK Plus Prestained Protein Ladder in different electrophoresis conditions are listed below:
kDa
~90~70
~175~130
~60
~50
~40
~10
~30
~20
~15
kDa
~80
~61
~165~120
~52
~42
~32
~10
~24
~17
~13
kDa
~82
~62
~165~125
~55
~45
~34
~7
~26
~16
~12
Tris-Glycine15%
Bis-Tris 4~12%MES buffer
Bis-Tris 4~12%MOPS buffer
GeneDireX, [email protected]
% ofmigration
0 %8 %
175130
9090
175
10 % 12 % 15 % 4-20 % MOPS MES TA
10 %20 %30 %40 %
50 %60 %
70 %80 %90 %
100 %
Tris Glycine Gel 4-12%Bis Tris Gel
3-8%Tris
Acetate
7060
50
30
40
130
7060
50
30
20
40
175130
40
90
10
130175
706050
30
20
15
40
90
10
130175
706050
30
20
15
10
32
80
131724
425261
120165
7
34
82
121626
455562
165
85
40
160115
655545
27181615
85
38
165125
706048
25
1915
11
10
40
90
15
20
30
5060
70125
TG
EVOgel
Note:1. The apparent molecular weight of each protein has been determined by calibration against unstained protein standards2. Supplemental data should be considered for more accurate adjustment in different electrophoresis conditions.All products are for research use only.Caution: Not intended for human or animal diagnostic or therapeutic uses.
