Fill yourself in on reagents...
1
Fill yourself in on reagents... Every one of the reagents here is new. Recently synthesized in our laboratories, one or more oi them could be the reagent you've been looking for. For determination of serum albumin. ο - [ (p -Hydroxyphenyl) azo ] benzoic Acid (EASTMAN 10614) (also known as HABA) HABA dye binds with serum albumin in phosphate buffer at pH 6.2 to 6.4 to form a colored complex with absorbance at 485nm. The reaction follows Beer's law over a wide range of concentrations. [Clin. Chim. Acta, 12,532 (1965)]. For phosphorylation. 2-Cyanoethyl Phosphate Di(cyclohexylamine Salt) (EASTMAN 10964) Monophosphate esters of nucleotides are readily pre- pared by using 2-cyanoethyl phosphate di(cyclohexyl- amine salt). For example, thymidine-3'-phosphate is prepared from 5'-0-tritylthymidine by condensation, followed by acid hydrolysis, then mild alkaline hy- drolysis. [J.A.C.S., 83, 159 (1961)]. Similarly, steroid 21-phosphate esters are synthesized by using 2-cyano- ethyl phosphate. [J.A.C.S., 85, 1118 (1963)]. For reduction of polycyclic quinones. Aluminum Cyclohexoxide (1M in Cyclohexanol) (EASTMAN A10941) Polycyclic quinones are converted to hydrocarbons un- der reflux with aluminum cyclohexoxide; pentacene- 6,13-quinone yields pure pentacene. The starting qui- none is readily prepared from phthalaldehyde and 1,4-cyclohexanedione. [Tetrahedron Letters, N o . 1, 5 (I960)]. For reduction, debromination, and synthesis of cyclopropanes. Zinc-Copper Couple (EASTMAN 11122) Phthalide is prepared from phthalimide in 70% yield by using zinc-copper couple in aqueous alkali, followed by acidification and crystallization. [Org. Syn., Coll. Vol. 2, 526 (1943)]. 1,4-Dibromo-l-phenylpropane is debrominated with a mixture of zinc-copper couple and dimethylformamide to yield cyclopropylbenzene. [Org. Syn., 44, 30 (1964)]. A number of cyclopropanes are prepared by the Simmons-Smith reaction of olefins and methylene iodide with zinc-copper couple. [/. Org. Chem., 24, 1825 (1959) and /. Am. Chem. Soc, 80, 5323 (1958)]. We also offer some new reagents with only a few com- ments. For synthesis of difluorocarbene : Lithium Trifluorace- tate (EASTMAN 10953). For preparing t-BOC-amino acids: tert-Butyl 2,4,5- Trichlorophenyl Carbonate (EASTMAN 10914). For N-blocking of amino acids: Benzyl p-Nitrophenyl Carbonate (EASTMAN 10942). For cleaving N-protective phthaloyl groups in protein synthesis: Hydrazine Acetate (EASTMAN 11019). For selective formylation of terminal amine group of ornithine and lysine : p-Nitrophenyl Formate (EASTMAN 11008). For protection of hydroxyl and amine groups in syn- thesis of glycerides, steroids, and nucleosides: 2,2,2- Trichloroethyl Chloroformate (EASTMAN 11050). For preparation of BhOC amino acids useful in solid- phase peptide synthesis: Benzhydryl Azidoformate (EASTMAN 11059). GLC derivatizing reagent: Boron Fluoride (14% in Propanol) (EASTMAN A10980). Dye laser, biological stain (C.I. #45160), TLC visuali- zation reagent: Rhodamine 6G (EASTMAN 10724). Titrant for non-aqueous titrimetry: Tetrabutylam- monium Hydroxide Titrant [ 0.1 M in benzine-methanol (9:1)] (EASTMAN A10934). For protein synthesis: Twenty N-blocked amino acids along with a number of other new reagents are listed in Kodak Publication JJ-161, Supple- mental List of Reagents for Protein Synthesis and Structure Determination. Use the coupon below to order your copy. All of these new chemicals are available from the Eastman Organic Chemicals dealer nearest you. For a copy of the EASTMAN Organic Chemicals Catalog List 45, listing more than 6,000 reagents, and to receive periodic supplements to it automatically, simply com- plete and return the coupon. B&A CURTIN FISHER HOWE & FRENCH NORTH-STRONG PREISER SARGENT-WELCH WILL Dept. 412L Eastman Organic Chemicals Eastman Kodak Company, Rochester, N.Y. 14650 Π Send JJ-1, "EASTMAN Organic Chemicals" catalog Π Send JJ-161, "Protein Reagents Supplement" Name Address City State Zip Kodak C&EN 77
Transcript of Fill yourself in on reagents...
Fill yourself in on reagents... Every one of the reagents here is new. Recently synthesized in our laboratories, one or more oi them could be the reagent you've been looking for.
F o r d e t e r m i n a t i o n of s e r u m a l b u m i n . ο - [ (p -Hydroxyphenyl) azo ] benzoic Acid ( E A S T M A N 10614) (also known as H A B A ) H A B A dye binds with serum albumin in phosphate buffer at pH 6.2 to 6.4 to form a colored complex with absorbance at 485nm. The reaction follows Beer's law over a wide range of concentrations. [Clin. Chim. Acta, 12,532 (1965) ] .
F o r p h o s p h o r y l a t i o n . 2-Cyanoethyl Phosphate Di(cyclohexylamine Salt) ( E A S T M A N 10964)
Monophosphate esters of nucleotides are readily prepared by using 2-cyanoethyl phosphate di(cyclohexyl-amine sal t ) . For example, thymidine-3'-phosphate is prepared from 5'-0-tritylthymidine by condensation, followed by acid hydrolysis, then mild alkaline hydrolysis. [J.A.C.S., 83, 159 (1961) ] . Similarly, steroid 21-phosphate esters are synthesized by using 2-cyanoethyl phosphate. [J.A.C.S., 85, 1118 (1963) ] .
F o r r e d u c t i o n of po lycyc l ic q u i n o n e s . Aluminum Cyclohexoxide (1M in Cyclohexanol) ( E A S T M A N A10941) Polycyclic quinones are converted to hydrocarbons under reflux with aluminum cyclohexoxide; pentacene-6,13-quinone yields pure pentacene. The starting qui-none is readily prepared from phthalaldehyde and 1,4-cyclohexanedione. [Tetrahedron Letters, N o . 1, 5 ( I 9 6 0 ) ] .
F o r r e d u c t i o n , d e b r o m i n a t i o n , a n d s y n t h e s i s of c y c l o p r o p a n e s . Zinc-Copper Couple ( E A S T M A N 11122) Phthalide is prepared from phthalimide in 70% yield by using zinc-copper couple in aqueous alkali, followed by acidification and crystallization. [Org. Syn., Coll. Vol. 2, 526 (1943) ] . 1,4-Dibromo-l-phenylpropane is debrominated with a mixture of zinc-copper couple and dimethylformamide to yield cyclopropylbenzene. [Org. Syn., 44, 30 (1964) ] . A number of cyclopropanes are prepared by the Simmons-Smith reaction of olefins and methylene iodide with zinc-copper couple. [/. Org. Chem., 24, 1825 (1959) and / . Am. Chem. Soc, 80, 5323 (1958) ] .
We also offer some new reagents with only a few comments. For synthesis of difluorocarbene : Lithium Trifluorace-tate ( E A S T M A N 10953). For preparing t -BOC-amino acids: tert-Butyl 2,4,5-Trichlorophenyl Carbonate ( E A S T M A N 10914). For N-blocking of amino acids: Benzyl p-Nitrophenyl Carbonate ( E A S T M A N 10942). For cleaving N-protective phthaloyl groups in protein synthesis: Hydrazine Acetate ( E A S T M A N 11019). For selective formylation of terminal amine group of ornithine and lysine : p-Nitrophenyl Formate ( E A S T M A N 11008). For protection of hydroxyl and amine groups in synthesis of glycerides, steroids, and nucleosides: 2,2,2-Trichloroethyl Chloroformate ( E A S T M A N 11050). For preparation of BhOC amino acids useful in solid-phase peptide synthesis: Benzhydryl Azidoformate ( E A S T M A N 11059). GLC derivatizing reagent: Boron Fluoride (14% in Propanol) ( E A S T M A N A10980). D y e laser, biological stain (C.I. # 4 5 1 6 0 ) , TLC visualization reagent: Rhodamine 6G ( E A S T M A N 10724). Titrant for non-aqueous titrimetry: Tetrabutylam-monium Hydroxide Titrant [ 0.1 M in benzine-methanol (9 :1 ) ] ( E A S T M A N A10934).
For protein synthesis: Twenty N-blocked amino acids along with a number of other new reagents are listed in Kodak Publication JJ-161, Supplemental List of Reagents for Protein Synthesis and Structure Determination. Use the coupon below to order your copy.
All of these new chemicals are available from the Eastman Organic Chemicals dealer nearest you. For a copy of the EASTMAN Organic Chemicals Catalog List 45, listing more than 6,000 reagents, and to receive periodic supplements to it automatically, s imply complete and return the coupon.
B&A C U R T I N F I S H E R H O W E & F R E N C H
N O R T H - S T R O N G P R E I S E R S A R G E N T - W E L C H WILL
Dept. 412L Eastman Organic Chemicals Eastman Kodak Company, Rochester, N.Y. 14650
Π Send JJ-1, "EASTMAN Organic Chemicals" catalog Π Send JJ-161, "Protein Reagents Supplement"
Name
Address
City
State Zip
Kodak
C&EN 77
![PRODUCT INSERT - Trinity Biotech...Store all reagents at 2-8 C. Reagents are ready for use after equilibration to room temperature. Materials provided [REF] 11 16 ANCA Kit (ethanol)](https://static.fdocument.org/doc/165x107/611a21c3cfe46762924da8e4/product-insert-trinity-biotech-store-all-reagents-at-2-8-c-reagents-are-ready.jpg)
