Fig. 1

8
Fig. 1 b c (µM) CG200745 Acetyl-histone H3 β- actin - 0. 1 1 10 SAHA 0 - CG200745 Acetyl-histone H3 β- actin + + + + + + + 0.5 1 2 4 8 1 6 2 4 (h) a

description

Fig. 1. a. b. 0. 0.5. 1. 2. 4. 8. 16. 24. (h). -. +. +. +. +. +. +. +. CG200745. Acetyl-histone H3. β -actin. c. -. (µM). 0.1. 1. 10. CG200745. SAHA. Acetyl-histone H3. β -actin. Fig. 2. a. 4. 8. 16. 24. 16. (h). 0. 1. 2. -. +. +. +. +. +. - PowerPoint PPT Presentation

Transcript of Fig. 1

Page 1: Fig. 1

Fig. 1

b

c

(µM)CG200745

Acetyl-histone H3

β-actin

- 0.1

1 10 SAHA

0-CG200745

Acetyl-histone H3

β-actin

+ + + + + + +0.5 1 2 4 8 16 24 (h)

a

Page 2: Fig. 1

Fig. 2

a

b

p53

0

CG200745 - 1 2 4 8 16 24 (h)

+ + + + + + SAHA

16

β-actin

p21Waf1/Cip1

Mdm2

c

Page 3: Fig. 1

Fig. 3

ba* *p<0.05

RC10.1RKO

*

*

*p<0.05*

*

*

RC10.1RKO

Page 4: Fig. 1

a

p53

0

β-actin

05 5 1010 20 20 4040 60 60 (min)

8hr+CHX 8hr+CHX+CG200745

b

Fig. 4

8hr+CHX8hr+CHX+CG200745

Page 5: Fig. 1

Fig. 5

b

p53

β-actin

K320R

K373R

K382R

Vectorp53 mutants

- - + - - + + - + -

- - - + - + - + + -

- - - - + - + + + -

a

CG200745 InputIgG

IP: p53

IB: Ac-p53(K320)

IB: Ac-p53(K373)

IB: Ac-p53(K382)

0

-

1 2 4 8 16 24 (hr)

+ + + + + + SAHA

16

IB: p53

C p53 WT

Page 6: Fig. 1

c

Fig. 5 (continued)

p53 mutants

No treatment

CG200745 (10 μM)

K320R

K373R

K382R

- - + - - + + - +

- - - + - + - + +

- - - - + - + + +

C p53 WT

Page 7: Fig. 1

Fig. 6

No treatment CG200745 (10 μM)

p53 mutants

K320R

K373R

K382R

- - + - - + + - +

- - - + - + - + +

- - - - + - + + +

C p53 WT

Page 8: Fig. 1

Supplementary Fig. 1a

β-actin

p53 - + - + - + - + - +CG200745

vector

- + - + - + - + - + - +

Mdm2

p21Waf1/Cip1

K320R K373R K382RK320RK373R

K373RK382R

K320RK382R

K320RK373RK382R

K320R

K373R

K382R

Vectorp53 mutants

- - + - - + + - + -

- - - + - + - + + -

- - - - + - + + + -

K320R

K373R

K382R

Vectorp53 mutants

- - + - - + + - + -

- - - + - + - + + -

- - - - + - + + + -

b c

C p53 WT C p53 WT

C p53 WTp53 WT


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