Differential expression in genes involved in the NF-κβ pathway among asymptomatic HTLV-1 carriers...
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POSTER PRESENTATION Open Access Differential expression in genes involved in the NF-b pathway among asymptomatic HTLV-1 carriers and HAM/TSP patients in Peru Michael Talledo 1,2* , Jason Rosado 1,2 , Carolina Alvarez 2,3 , Daniel Clark 2,4 , Eduardo Gotuzzo 2,5,6 From 16th International Conference on Human Retroviruses: HTLV and Related Viruses Montreal, Canada. 26-30 June 2013 HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) is a neuroinflammatory, non-remitting and disabling disease. NF-b pathway plays a role in the patho- genesis of this condition. We compare the expression at peripheral level of84 genes involved in the NF-b pathway among asymptomatic HTLV-1 carriers (AC) and HAM/ TSP patients. mRNA from PBMCs was isolated from 12 HTLV-1 carriers classified into three groups: fourAC (=patients without any neurological condition) and eight patients with HAM/TSP (four with EDSS scores of 1-5 and four with EDSS score of 5.5-9.0). 84 genes related to the NF-b pathway were evaluated using Superarray plates (SABiosciences) and Real Time PCR in both groups. Ct values under 35 were considered positive, False Discovery Rate was used to control by multiple comparisons. Three genes were dysregulated in HAM/TSP patients. The expression level of NF-bIA was higher in AC compared to HAM/TSP patients, while EGR1 and IL-8 showed a lower expression in AC compared to HAM/TSP patients (p<0.05 after multiple testing correction). These results are in agreement to our previous genetic findings; nevertheless, a validation in an independent group is required to confirm these results. Further studies evaluating the role of genes involved in the NF-b pathway as potential biomarkers for HAM/TSP are needed. Authors’ details 1 Laboratorio de Epidemiología Molecular, Universidad Peruana Cayetano Heredia, Lima, Peru. 2 Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru. 3 Rega Institute for Medical Research, Katholieke Universiteit Leuven, Leuven, Belgium. 4 Laboratorios de Investigación y Desarrollo, Universidad Peruana Cayetano Heredia, Lima, Peru. 5 Facultad de Medicina, Universidad Peruana Cayetano Heredia, Lima, Peru. 6 Hospital Nacional Cayetano Heredia, Lima, Peru. Published: 7 January 2014 doi:10.1186/1742-4690-11-S1-P126 Cite this article as: Talledo et al.: Differential expression in genes involved in the NF-b pathway among asymptomatic HTLV-1 carriers and HAM/TSP patients in Peru. Retrovirology 2014 11(Suppl 1):P126. Submit your next manuscript to BioMed Central and take full advantage of: • Convenient online submission • Thorough peer review • No space constraints or color figure charges • Immediate publication on acceptance • Inclusion in PubMed, CAS, Scopus and Google Scholar • Research which is freely available for redistribution Submit your manuscript at www.biomedcentral.com/submit * Correspondence: [email protected] 1 Laboratorio de Epidemiología Molecular, Universidad Peruana Cayetano Heredia, Lima, Peru Full list of author information is available at the end of the article Talledo et al. Retrovirology 2014, 11(Suppl 1):P126 http://www.retrovirology.com/content/11/S1/P126 © 2014 Talledo et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Transcript of Differential expression in genes involved in the NF-κβ pathway among asymptomatic HTLV-1 carriers...
POSTER PRESENTATION Open Access
Differential expression in genes involved in theNF-�b pathway among asymptomatic HTLV-1carriers and HAM/TSP patients in PeruMichael Talledo1,2*, Jason Rosado1,2, Carolina Alvarez2,3, Daniel Clark2,4, Eduardo Gotuzzo2,5,6
From 16th International Conference on Human Retroviruses: HTLV and Related VirusesMontreal, Canada. 26-30 June 2013
HTLV-1-associated myelopathy/tropical spastic paraparesis(HAM/TSP) is a neuroinflammatory, non-remitting anddisabling disease. NF-�b pathway plays a role in the patho-genesis of this condition. We compare the expression atperipheral level of84 genes involved in the NF-�b pathwayamong asymptomatic HTLV-1 carriers (AC) and HAM/TSP patients. mRNA from PBMCs was isolated from 12HTLV-1 carriers classified into three groups: fourAC(=patients without any neurological condition) and eightpatients with HAM/TSP (four with EDSS scores of 1-5 andfour with EDSS score of 5.5-9.0). 84 genes related to theNF-�b pathway were evaluated using Superarray plates(SABiosciences) and Real Time PCR in both groups. Ctvalues under 35 were considered positive, False DiscoveryRate was used to control by multiple comparisons. Threegenes were dysregulated in HAM/TSP patients. Theexpression level of NF-�bIA was higher in AC comparedto HAM/TSP patients, while EGR1 and IL-8 showed alower expression in AC compared to HAM/TSP patients(p<0.05 after multiple testing correction). These results arein agreement to our previous genetic findings; nevertheless,a validation in an independent group is required to confirmthese results. Further studies evaluating the role of genesinvolved in the NF-�b pathway as potential biomarkers forHAM/TSP are needed.
Authors’ details1Laboratorio de Epidemiología Molecular, Universidad Peruana CayetanoHeredia, Lima, Peru. 2Instituto de Medicina Tropical Alexander vonHumboldt, Universidad Peruana Cayetano Heredia, Lima, Peru. 3RegaInstitute for Medical Research, Katholieke Universiteit Leuven, Leuven,Belgium. 4Laboratorios de Investigación y Desarrollo, Universidad Peruana
Cayetano Heredia, Lima, Peru. 5Facultad de Medicina, Universidad PeruanaCayetano Heredia, Lima, Peru. 6Hospital Nacional Cayetano Heredia, Lima,Peru.
Published: 7 January 2014
doi:10.1186/1742-4690-11-S1-P126Cite this article as: Talledo et al.: Differential expression in genesinvolved in the NF-�b pathway among asymptomatic HTLV-1 carriersand HAM/TSP patients in Peru. Retrovirology 2014 11(Suppl 1):P126.
Submit your next manuscript to BioMed Centraland take full advantage of:
• Convenient online submission
• Thorough peer review
• No space constraints or color figure charges
• Immediate publication on acceptance
• Inclusion in PubMed, CAS, Scopus and Google Scholar
• Research which is freely available for redistribution
Submit your manuscript at www.biomedcentral.com/submit
* Correspondence: [email protected] de Epidemiología Molecular, Universidad Peruana CayetanoHeredia, Lima, PeruFull list of author information is available at the end of the article
Talledo et al. Retrovirology 2014, 11(Suppl 1):P126http://www.retrovirology.com/content/11/S1/P126
© 2014 Talledo et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative CommonsAttribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction inany medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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