develop or improve your hPLC Method - Brechbühler AG · Can be used as reversed- or normal-phase...

158 Phenomenex

HPLC

Luna

TM

Luna®

Try Gemini® C18 for even longer column lifetimes (see page 126).

Luna® is a registered trademark of Phenomenex, Inc.

Material CharacteristicsPacking Particle Pore Surface Carbon

CalculatedBonded pH

Material Shape/Size Size Area Load PhaseCoverage Stability (μm) (Å) (m2/g) % (µmole/m2)

LunaSilica(2)† Spher.3,5,10,15 100 400 0 – 1.5-7.0LunaC5 Spher.5,10 100 440 12.5 7.85 1.5-10LunaC8 Spher.5,10 100 440 14.75 5.50 1.5-10LunaC8(2) Spher.3,5,10,15 100 400 13.5 5.50 1.5-10LunaC18 Spher.5,10 100 440 19 3.00 1.5-10LunaC18(2) Spher.2.5,3,5,10,15 100 400 17.5 3.00 1.5-10LunaPhenyl-Hexyl Spher.3,5,10,15 100 400 17.5 4.00 1.5-10LunaCN† Spher.3,5,10 100 400 7.0 3.80 1.5-7.0LunaNH2

† Spher.3,5,10 100 400 9.5 5.80 1.5-11LunaSCX† Spher.5,10 100 400 BindingCapacity:0.15meq/g 2.0-7.0

USP Phases for Virtually Every Application Luna Bonded Phase Selectivity Chart

OnE Of thE wOrLd'S LEAding hPLC COLUMnS

develop or improve your hPLC Method• wide ph stability from 1.5 to 10†

• Exceptionally rugged USP Phases: Silica, C5, C8, C18, Phenyl-hexyl, Cn, nh2, SCX

• Easy method scalability with 3, 5, 10, 10-PREP and 15 μm medias

• Extensive batch traceability and reproducibility data supplied with every column

USP Column Classification

Phase

Description

Common applications

L1 C18(2) 2.5,3,5,10,10-PREP,15µmC18phase.Excellentefficiency,peakshapeandresolution.SlightlylowercarbonloadthanoriginalLunaC18.

Acetaminophen,Aspirin,Caffeine,Albuterol,AmitriptylineHydrochloride,Amoxicillin,Atenolol,Cephalexin,Cephradinecapsules,Chloramphenicol,CortisoneAcetate,Dextromethorphan,Diphenhydramine,Pseudoephedrine,Dopamine,Estradiol,Guaifenesin,Ibuprofen,SterileImipenem,Imipramine,Lidocaine,Lorazepam,Minoxidil,Naproxen,PhenylephrineHydrochloride,Phenylpropanolamine,Prednisoneoralsolution,Procainamide,Propoxyphene,Reserpine

L3 Silica(2) 3,5,10µmUltra-puresilicawithhighcolumnbedstabilityenhancedbyparticleshapeuniformity.

Alprazolam,Hydrocodonebitartrate,Hydrocortisone,FatSolubleVitamins,Phthalates,FattyAcids,Lutein,Lycopene,Estradiol

L7C8(2) 3,5,10,10-PREP,15µm

C8phaseforexcellentefficiency,peakshapeandresolution.SignificantlyimprovedperformanceovertraditionalC8phasesduetohighsurfacecoverage.

Doxepine,Doxylaminesuccinate,Fluoxetine,Glyburide,IbuprofenOralSuspension,Propranolol,Levonorgestrel,Ethinylestradiol,Melengestrolacetate,Glucosamine

L8 NH2 3,5,10µmAminophase.Canbeusedinreversedornormalphasemodes.StablefrompH1.5to11.0andunder100%aqueousconditions.Highperformancesilicaandbondingtechniquesproducesarugged,highlyreproduciblecolumn.

Simplesugars,Carboplatin,Lactuloseconcentrate,Levocarnitinetablets

L9 SCX 5,10µmABenzeneSulfonicAcidbondedphaseisusedtomakethisStrongCationExchange(SCX)column.Offersgreatpeakshapeandresolution.

Coughandcoldcompounds,Raclopride,SodiumAcetate,Erythromycin

L10 CN 3,5,10µmCyanophase.Canbeusedasreversed-ornormal-phasematerial.TheuseofLunabasesilicaresultsinoverallphasereproducibilityandperformance.

BenzalkoniumChloride,NortriptylineHClCapsules,Prednisolone,Tetracaine,Quinapriltablets

L11 Phenyl-Hexyl

3,5,10,10-PREP,15µmAphenylphasewhichemploysahexylalkyllinkerasopposedtothetraditionalpropylchain.Offersgreatstabilityaswellasalternativeselectivity.

Oxacillin,Captopril,Chlorpheniramine,Pseudoephedrine,MethadoneHydrochlorideOralConcentration

If Luna does not provide at least anequivalentseparationascomparedtoacompeting columnof the sameparticlesize,similarphaseanddimensions,sendinyourcomparativedatawithin45daysandkeeptheLunacolumnforFREE

159Phenomenex

HPLCLuna

TM

Luna®

LUnA SiLiCA

A Backbone and Phase designed for Long Column LifetimesLuna’s excellent performance is not simply the result of ultra-pure metal-free silica (99.99 % purity). Meticulous care is given to the quality control of surface smoothness, pore structure and pore consistency to ensure particles of uniform structure and enhanced mechanical strength. Either bonded or unbonded, Luna silica produces highly advanced HPLC columns:

• Low percentage of “fines” from damaged silica leading to lower backpressures and enhanced column performance and lifetimes

• high column bed stability enhanced by particle shape uniformity

incredible Silica SmoothnessLuna silica is extremely smooth and spherical. For bonded phases, this provides a uniform bonding surface for consistent and even bonded phase coverage. The likelihood of silica particle shearing and breakage during bonding and packing is very low; thus, Luna columns have high efficiencies and long column lifetimes.

Compare Surface Smoothness

Phe

nom

enex

Lun

a®

5 μm

C18

Wat

ers

Sym

met

ry®

5 μm

C18

Agi

lent

Tec

hnol

ogie

s Z

orb

ax®

5 μm

SB

-C18

natural Products Column: Luna5μmSilica(2)Dimensions: 150x4.6mmPart no.: 00F-4274-E0Mobile Phase: Hexane/Dioxane(85:15)Flow Rate: 1.5mL/minDetection: UV@230nmSample: 1. Dihydrokawain 2. Yangonin 3. Kawain 4. Dihydromethysticin 5. Methysticin

App

ID1

138

10 20 min0

1

2

3

4

5

Low High

Hydrophobicity

H-Bonding

AromaticSelectivity

SilanolActivity(pH2.5)

uSP: L3

Luna Silica(2)

—3µm,5µm,10μm,10μm-PREP,and15μmUnbondedsilicaNon-polarcompounds

pH StabilityParticleSize

PhaseApplication

n/A

n/A

n/A

• recommended for preparative and bulk packing into dAC systems, see page 288 for more information

see p. 379 for testing probes.

160 Phenomenex

HPLC

Luna

TM

Luna®

App

ID1

100

1

2

3Pyridine Asymmetry:As 2.27

0 2 4 6 8 10 12 min

0

100

200

300

1

2

3

Pyridine Adsorption:No elution

0 2 4 6 8 10 12 min

0

100

200

300

400

500

1

2

3

0 2 4 6 8 10 12 min

0

100

200

300

400 Pyridine Asymmetry:As 1.44

Zorbax® 5 μm C18

Symmetry® 5 μm C18

Luna® 5 μm C18(2)

Peak asymmetry Comparison of Competing Columns**

Conditions for all columns Dimensions: 150x4.6mmMobile Phase: Acetonitrile/Water(50:50)Flow Rate: 1.0mL/minDetection: UV@254nmSample: 1. Pyridine 2. Phenol 3. Toluene

Comparison of 7 different 5 μm reversed phase columns. This survey measures the degree of silanol activity on the surface of each silica. In this survey, Luna 5 µm C18(2) material demonstrates the lowest silanol activity.

Pyridine Peak asymmetry Comparison**

LUnA C18(2), C8(2), C5

take One Small Step and a giant Leap in PerformanceLuna has found a place as one of the World’s top reversed phase columns because it provides a measurable improvement over many HPLC columns for two important chromatographic properties: resolution and peak shape. The high efficiencies and bonded phase surface coverage provide for sharp peaks. The result:

• free exposed silanols virtually eliminated by complete bonding and endcapping

• Sharp peak shape for good method sensitivity

• ph stable from 1.5 to 10.0 for over 1,000 hours

** Thecomparativedatapresentedheremaynotberepresentative forallapplications.

Hydrophobicity

H-Bonding

AromaticSelectivity


1.5-10.05μm,10μmC5,endcappedSmallmoleculeswhenlessretentionandgreaterspeedisdesiredGreaterhydrolyticandpHstabilitycomparedtomostC4phases


PhaseApplication

Strength

Low High

LC/MS Certified

Luna C5

1.5-10.02.5µm,3μm,5μm,10μm,10μm-PREP,and15μmC18,endcappedSmallmoleculesWidepHstabilityprovideslongercolumnlifetimeandgreatermethodflexibility


PhaseApplication

Strength

Low High

Hydrophobicity

H-Bonding

AromaticSelectivity


uSP: L1

LC/MS Certified

Luna C18(2)

1.5-10.03μm,5μm,10μm,10μm-PREP,and15μmC8,endcappedSmallmoleculeswhenlessretentionandgreaterspeedisdesiredLowersilanolactivitythanC18(2)phasepluswidepHstabilityforlongercolumnlifeandgreatermethodflexibility


PhaseApplication

Strength

Low High

Hydrophobicity

H-Bonding

AromaticSelectivity


uSP: L7

LC/MS Certified

Luna C8(2)




161Phenomenex

HPLCLuna

TM

Luna®

2 4 6 8 10 12 14 16 min

1

2

3

4

5

0

Steroids Column: Luna5μmC18(2)Dimensions: 150x4.6mmPart no.: 00F-4252-E0Mobile Phase: 0.1%H3PO4/Acetonitrile/Methanol(54:35:11)Flow Rate: 0.75mL/minDetection: UV@254nmSample: 1. Hydrocortisone 3. 11-α-Hydroxyprogesterone 2. Corticosterone 4. CortisoneAcetate 5. 11-Ketoprogesterone

App

ID1

185 Polynuclear Aromatic hydrocarbons:

EPA Method 8310 Column: Luna5μmC18(2)Dimensions: 250x4.6mmPart no.: 00G-4252-E0Mobile Phase: A:WaterB:AcetonitrileGradient: A/B(25:75)to100%Bin25minFlow Rate: 2.0mL/minTemperature: 22°CDetection: UV@254nmSample: 1.Naphthalene 5.Anthracene 9. Chrysene 2.Acenaphthylene 6.Fluoranthene 10. Benzo[e]pyrene 3.Fluorene 7.Pyrene 11. Benzo[b]fluoranthene 4.Phenanthrene 8.Benz[a]anthracene 12. Benzo[k]fluoranthene 13. Benzo[a]pyrene 14. Dibenz[a,h]anthracene 15. Benzo[g,h,i]perylene 16. Indenol[1,2,3-c,d]pyrene

App

ID1

4269

2 4 6 8 10 12 min

3

1

2

0

Acetaminophen, USP Method Column: Luna5μmC18(2)Dimensions: 150x4.6mmPart no.: 00F-4252-E0Mobile Phase: Water/Methanol/AceticAcid(69:28:3)Flow Rate: 1.5mL/minDetection: UV@275nmSample: 1. Acetaminophen 2. Caffeine 3. BenzoicAcid

App

ID1

293water Soluble Vitamins

Column: Luna5μmC18(2)Dimensions: 150x4.6mmPart no.: 00F-4252-E0Mobile Phase: 20mMPotassiumPhosphate,pH3.0/Acetonitrile(95:5)Flow Rate: 1.5mL/minTemperature: 22°CDetection: UV@214nmSample: 1. Thiamine 4. Pantothenicacid 2. Cyanocobalamin(VitaminB12) 5. Niacinamide 3. Ascorbicacid 6. p-Aminobenzoicacid

App

ID3

305

Pharmaceutical Preservatives Column: Luna5μmC5Dimensions: 150x4.6mmPart no.: 00F-4043-E0Mobile Phase: A:0.5%Aceticacidinwater/acetonitrile(80:20) B:0.5%Aceticacidinwater/acetonitrile(20:80)Gradient: A/B(100:0)toA/B(0:100)in30minFlow Rate: 1mL/minTemperature: 25°CDetection: UV@254nmSample: 1. Propylparabenimpurity 2. Benzylalcohol 3. Phenol 4. Benzoicacid 5. Methylparaben 6. Benzaldehyde 7. Ethylparaben 8. Propylparaben

App

ID1

141 Penicillin Antibiotics

Column: Luna5μmC8Dimensions: 150x4.6mmPart no.: 00F-4040-E0Mobile Phase: Methanol/25mMKH2P04,pH4.6(55:45)Flow Rate: 1mL/minTemperature: 22°CDetection: UV@254nmSample: 1. Amoxicillin 2. Ampicillin 3. PenicillinG 4. PenicillinV

App

ID1

215

Luna C18(2), C8(2), C5 (cont'd)

162 Phenomenex

HPLC

Luna

TM

Luna®

min0 2

3

2

1

4

4

5

6 8 10 12

Adenine family Column: Luna5μmC18(2)Dimensions: 150x4.6mmPart no.: 00F-4252-E0Mobile Phase: A:20mMAmmoniumacetate,pH4.5 B:AcetonitrileGradient: A/B(97:3)to(60:40)in10minFlow Rate: 1mL/minTemperature: AmbientDetection: UV@260nmSample: 1. ATP 4. Adenine 2. ADP 5. Adenosine 3. AMP

App

ID1

5756

� 0 �� 10 �� 20 � 25

1

2

3

4

5

6

min

7

8

Saw Palmeto Berry, p-Bromophenacyl Esters Column: Luna3μmC8(2)Dimensions: 150x4.6mmPart no.: 00F-4248-E0Mobile Phase: Acetonitrile/Water(87:13)Flow Rate: 1.5mL/minTemperature: 25°CDetection: UV@254nmSample: 1. CapricAcid 5. LinoleicAcid 2. LauricAcid 6. PalmiticAcid 3. LinolenicAcid 7. OleicAcid 4. MyristicAcid 8. StearicAcid

App

ID1

072

0 2 4 6 8 10 min

1

2 3

4

5

glucocorticoids Column: Luna5μmC8Dimensions: 150x4.6mmPart no.: 00F-4040-E0Mobile Phase: Acetonitrile/Water(50:50)Flow Rate: 1.0mL/minDetection: UV@240nmSample: 1. Dexamethasone21-Phosphate 2. Prednisolone 3. Dexamethasone 4. Prednisolone21-Acetate 5. Betamethasone17-Valerate

App

ID1

284

Polyamines (dansylated) Column: Luna5μmC18+SecurityGuardDimensions: 250x4.6mmPart no.: 00G-4252-E0andKJ0-4282andAJ0-4287Mobile Phase: A:93%MethanolinWater B:65%AcetonitrileinWaterGradient: A/B(0:100)toA/B(100:0)in28mintoA/B(0:100)in40minutesTemperature: 35°CFlow Rate: 1.5mL/minDetection: λEX360nm,λEM530nmSample: 1. Putrescine 5. Spermine 2. DHX 6. Homospermine 3. DHP 7. Methylethylhomospermine 4. Spermidine 8. Diethylhomospermine(DEHOP)

App

ID1

288

Sun Screens Column: Luna5μmC18Dimensions: 250x4.6mmPart no.: 00G-4041-E0Mobile Phase: Methanol/Water(80:20)Flow Rate: 1.5mL/minTemperature: 22°CDetection: UV@240nmSample: 1. Benzophenone3 2. DCHP 3. ParsolMCX 4. Homosalate2 5. Octylsalicylate 6. Homosalate1 7. Homosalate

App

ID1

239

hormones Column: Luna5μmC18Dimensions: 100x4.6mmPart no.: 00D-4041-E0Mobile Phase: Water/Tetrahydrofuran/Acetonitile(60:22.5:17.5)Flow Rate: 1.2mL/minTemperature: 40°CDetection: UV@244nmSample: 1. Norgestrel 5. syn-Norgestimate 2. syn-Norgestreloxime 6. syn-Norgestreloximediacetate 3. anti-Norgestreloxime 7. anti-Norgestimate 4. Norgestrelacetate 8. anti-Norgestreloximediacetate

App

ID9

903

Luna C18(2), C8(2), C5 (cont'd)

163Phenomenex

HPLCLuna

TM

Luna®

App

ID1

0823

App

ID1

201

5 10 15 20 min

12

3

45

67

8

Luna® 5 μm Phenyl-Hexyl

App

ID1

0822

Waters Spherisorb® 5 μm Phenyl

App

ID1

0821 Agilent Technologies

Zorbax® 5 μm SB-Phenyl

Agilent Technologies Zorbax® 5 μm

Phenyl

Chromatographic Comparisons of Phenyl Columns**

LUnA PhEnyL-hEXyL

Enhanced SelectivityLuna Phenyl-Hexyl provides separations not achievable on C18 or C8 columns; such as increased retention for polar, aromatic compounds as well as reversals in analyte elution order. Luna Phenyl-Hexyl is a reproducible, extremely stable phenyl phase. Most phenyl phases use a short propyl (3 carbon) linker, which limits phase stability. The Phenyl-Hexyl bonded phase employs a phenyl ring with a hexyl (6 carbon) linker and is densely bonded to Luna silica surface. Dense bonding and the hexyl linker reduce bonded phase hydrolysis and increases chemical stability. The result:

• highly reproducible and stable phenyl phase

• dual selectivity of both phenyl phase and a short alkyl phase (C5 or C8)

• Excellent retention of aromatic and polar, amine compounds

• 1.5 to 10 ph stability for over 6000 hours

**Thecomparativedatapresentedheremaynotberepresentativeforallapplications.

Antibacterials Conditionsforallcolumns:Dimensions: 150x4.6mmMobile Phase: A: 20mMKH2PO4,pH2.5 B: AcetonitrileGradient: StartA/B(80:20)toA/B(75:25)in 5min,gotoA/B(55:45)in15minFlow Rate: 1.0mL/minDetection: UV@254nmSample: 1. Carbadox 5. Sulfadimethoxine 2. Thiamphenicol 6. Sulfaquinoxaline 3. Furazolidone 7. NalidixicAcid 4. OxolinicAcid 8. PiromidicAcid

see p. 379 for testing probes.1.5-10.03μm,5μm,10μm,10μm-PREP,and15μmPhenylwithHexyl(C6)linker,endcappedNon-polarcompoundsAromaticselectivityenhancedbyhigherhydrophobicityduetohexyllinker


PhaseApplication

Strength

Low High

Hydrophobicity

H-Bonding

AromaticSelectivity


uSP: L11

LC/MS Certified

Luna Phenyl-hexyl

164 Phenomenex

HPLC

Luna

TM

Luna®

0 2 4 6 8 min

15859

1 2

3

4

food Additives Column: Luna5μmPhenyl-HexylDimensions: 150x4.6mmPart no.: 00F-4257-E0Mobile Phase: A:50mMKH2PO4+0.1%H3PO4 B:AcetonitrileGradient: A/B(75:25)toA/B(25:75)in18min, holdatA/B(25:75)for12minFlow Rate: 1mL/minTemperature: 22°CInjection: 20µLDetection: UV@230nmSample: 1. Saccharin 5. Dehydroaceticacid 2. p-Hydroxybenzoicacid 6. p-Toluicacid 3. Sorbicacid 7. p-Hydroxybenzoicacidethylester 4. p-Hydroxybenzoicacid 8. n-Propylp-hydroxybenzoate methylester

App

ID1

199

Cough and Cold Medicine Column: Luna3μmPhenyl-HexylDimensions: 75x4.6mmPart no.: 00C-4256-EOMobile Phase: A:Acetonitrile B:20mMKH2PO4/Methanol(80:20)pH9.0Gradient: A/B(0:100)toA/B(80:20)in5minFlow Rate: 1mL/minTemperature: 22°CInjection: 20µLDetection: UV@214nmSample: 1. p-Aminophenol 5. Butylparaben 2. Benzoicacid 6. Chlorpheniramine 3. Acetaminophen 7. Diphenhydramine 4. Pseudoephedrine 8. Dextromethorphan

App

ID2

572

Luna Phenyl-hexyl (cont'd)

Enhance Aromatic Selectivity with Methanol!Luna Phenyl-Hexyl can provide separations not achievable on alkyl phases due to an increased retention for aromatic compounds. To fully express this aromatic selectivity, varying amounts of methanol can be used as the organic mobile phase in place of acetonitrile. π-π interactions between the bonded phase and different analytes can be modulated by the amounts of acetonitrile and methanol used in the mobile phase.

goldenseal Extract - Methanol Effects Columns: Luna5μmC18(2) Luna5μmPhenyl-HexylDimensions: 150x4.6mmMobile Phase: 20mmPotasiumPhosphate,pH2.5/Methanol(50:50)Flow Rate: 1mL/minDetection: UV@254nmSample: 1. Hydrastatine 2. Berberine

Luna 5 µm C18(2)

Luna 5 μm Phenyl Hexyl

1

15472

2

0 2 4 min

1

15471

2

0 2 4 min

App

ID1

5471

App

ID1

5472

Dramatic increase in retention with high organic due to aromatic selectivity

tricyclic Antidepressants Column: Luna5μmPhenyl-HexylDimensions: 150x4.6mmPart no.: 00F-4257-EOMobile Phase: 20mMPhosphatebufferpH7.0/ Acetonitrile/Methanol(30:35:35)Flow Rate: 1.5mL/minDetection: UV@254nmSample: 1. Nortriptyline 3. Amitriptyline 2. Imipramine 4. Clomipramine

App

ID1

5859

165Phenomenex

HPLCLuna

TM

Luna®

Agilent Technologies® Zorbax® 5 μm SB-CN

Waters® Spherisorb® 5 μm CN

Cn Column Comparsion**

Luna® 5 μm CNBatch-to-Batch reproducibility Column: Luna®5μmCNDimensions: 150x4.6mmMobile Phase: A:Hexane,B:Methylenechloride/Methanol(80:20),A/B(80:20)Flow Rate: 2.0mL/minDetection: UV@254nmInjection: 1.0µLTemperature: AmbientSample: 1. Hydrocortisone 2. Prednisone 3. Cortisone 4. HydrocortisoneAcetate

App

ID3

464

Column-to-Column reproducibility Column: Luna®5μmCNDimensions: 150x4.6mmMobile Phase: A:Hexane,B:Methylenechloride/Methanol(80:20),A/B(95:5)Flow Rate: 1.0mL/minInjection: 5µLDetection: UV@254nmTemperature: AmbientSample: 1. Dimethylphthalate 2. Diethylphthalate 3. Dibutylphthalate 4. Dioctylphthalate

App

ID3

466

normal Phase Conditions Conditionsforallcolumns:Dimensions: 150x4.6mmMobile Phase: A:Hexane,B:Methylenechloride/Methanol(80:20),A/B(99:1)Flow Rate: 1.0mL/minDetection: UV@254nmTemperature: AmbientSample: PhthalateEsters 1. Di-n-octylphthalate 2. Bis(2-Ethylhexyl)phthalate 3. Butylbenzylphthalate 4. Di-n-butylphthalate 5. Diethylphthalate 6. Dimethylphthalate

App

ID3

471

** Thecomparativedatapresentedheremaynotberepresentative forallapplications.

LUnA Cn (CyAnO)

Proven reproducibilityFor carboxyl, carbonyl and amine containing compounds, Luna CN offers a unique polar selectivity in reversed phase and normal phase mode. Luna CN provides sharp peaks and great reproducibility run-to-run, column-to-column and batch-to-batch. Luna’s smooth silica allows for a more uniform bonding with improved resistance to bonded phase hydrolysis to produce one of the most stable CN phases. The result:

• improved peak shape

• One of the most stable Cn columns under reversed phase or normal phase conditions.

• ph stable from 1.5 to 7.0

1.5-7.03μm,5μm,and10μmCyano,endcappedCompoundswithCOOH,CO,NH2,NHR2,orNR2

Improvedreproducibilityformoreconsistentresultsrun-to-run,column-tocolumn,batch-to-batch


PhaseApplication

Strength

Low High

Hydrophobicity

H-Bonding

AromaticSelectivity


uSP: L10

Luna Cn

reversed Phase Conditions Column: Luna®5μmCNDimensions: 150x4.6mmMobile Phase: A:Acetonitrile,B:20mMPotassiumphosphatepH4.0,A/B(35:65)Flow Rate: 1.0mL/minDetection: UV@210nmSample: TricyclicAntidepressants 1. Doxepin 2. Desipramine 3. Nortriptyline 4. Amitriptyline 5. Protriptyline

App

ID1

2958


2

1

3

2

54

4 6 8 10 12 14 16 18 min0

166 Phenomenex

HPLC

Luna

TM

Luna®

12-18mer poly-dt Oligonucleotide Column: Luna5μmNH2Dimensions: 150x4.6mmPart no.: 00F-4378-E0Mobile Phase: A) 20mMSodiumPhosphate,10%Acetonitrile,pH7.0 B) 20mMSodiumPhosphate,10%Acetonitrile, 1MSodiumChloride,pH7.0Gradient: A/B(75:25)toA/B(5:95)in50minutesFlow Rate: 1mL/minTemperature: 40°CDetection: UV@260nmSample: 12-18merpoly-DToligonucleotidestandard

App

ID1

4628

40 min0 20 30

4 65327

1

Simple Sugars Column: Luna5μmNH2Dimensions: 250x4.6mmPart no.: 00G-4378-E0Mobile Phase: Acetonitrile/Water(80:20)Flow Rate: 3mL/minTemperature: 40°CDetection: RISample: 1. Xylose 5. Maltose 2. Fructose 6. Melezitose 3. Glucose 7. Raffinose 4. Sucrose

App

ID1

4285

Steroids Column: Luna5μmNH2Dimensions: 250x4.6mmPart no.: 00G-4378-E0Mobile Phase: Hexane/Ethanol(85:15)Flow Rate: 2mL/minTemperature: 22°CDetection: UV@240nmSample: 1. 11-Ketoprogesterone 4. Prednisolone21-Acetate 2. 11-Hydroxyprogesterone 5. Cortisone 3. CortisoneAcetate 6. Prednisolone

App

ID1

4299

LUnA nh2 (AMinO)

developed for ruggednessLuna NH2 retains hydrogen-bonding compounds under three separation modes: Reversed Phased, Normal Phase, and Ion Exchange. Luna NH2 provides reproducible retention and selectivity with improved column lifetime. Amino columns can be problematic as the bonded phase easily hydrolyses off the silica, shortening retention time over the life of the column. The bonded phase stability of Luna NH2 is illustrated by 1.5 to 11.0 pH stability and 100 % aqueous mobile phase stability. The result:

• increased bonded phase stability improves reproducibility and column lifetime

• Excellent retention of simple sugars, complex sugars, sugar alcohols and other hydrogen-bonding compounds under reversed Phase, normal Phase, ion Exchange conditions

• ph stable from 1.5 to 11.0

• Stable in 100 % aqueous mobile phases

Stability in 100 % Aqueous Mobile Phase Column: Luna5μmNH2Dimensions: 250x4.6mmPart no.: 00G-4378-E0Mobile Phase: 20mMPotassiumPhosphateBufferpH2.7Flow Rate: 1.5mL/minDetector: UV@254nmTemperature: AmbientInjection: 2.5µLConditions: Continuouslyflushedat1.0mL/minusing100%20mMPotassium PhosphateBufferpH2.7betweeninjections

1.5-11.03μm,5μm,and10μmAminoCompoundswithCOOH,CO,NH2,NHR2,orNR2

Sugarsbyreversedphase,steroidsbynormalphase,oligonucleotidesbyionexchange


PhaseApplication

Strength

Low High

Hydrophobicity

H-Bonding

AromaticSelectivity


uSP: L8

Luna nh2

n/A


167Phenomenex

HPLCLuna

TM

Luna®

min2

1

2

3

0 4 6 8 10 12

0 100 200 300 400

10

15

0

5

-5

20

25

30

35

Ret

entio

n T

ime

(min

)

Stability Time (hours)

CMPUMP

Stability of Luna Amino at ph 1.5Column: Luna5µmNH2 Dimensions: 250x4.6mmPart no.: 00G-4378-E0Mobile Phase: 20mMPotassiumPhosphateBuffer pH2.7/Acetonitrile(95:5)Flow Rate: 1.5mL/minDetector: UV@254nmTemperature: AmbientStability Conditions: Continuouslyflushedat1.0mL/min using20mMPotassiumPhosphateBuffer pH1.5/Acetonitrile(95:5)betweeninjections

Stability of Luna Amino at ph 11.0Column: Luna5µmNH2Dimensions: 250x4.6mmPart no.: 00G-4378-E0Mobile Phase: 20mMPotassiumPhosphateBuffer pH2.7/Acetonitrile(95:5)Flow Rate: 1.5mL/minDetector: UV@254nmTemperature: AmbientStability Conditions: Continuously flushed at 1.0 mL/min using 20 mM Potassium Phosphate Buffer pH 11.0/Acetonitrile (95:5) between injections

Luna NH2 (cont'd)

Method flexibilityStable from pH 1.5 to 11.0, Luna Amino offers greater flexibility in method development. By adjusting the pH of the mobile phase, retention times of analytes can often be manipulated - depending on the pKa of the sample analyte. Even if extreme pH is not required, this pH stability indicates column ruggedness and generally longer column life.

Phospholipids in hiLiC mode Column: Luna5μmNH2Dimension: 150x4.6mmPart no.: 00F-4378-E0Mobile Phase: A:Water,B:AcetonitrileGradient: A/B(5:95)to(50:50)in15minFlow Rate: 1mL/minTemperature: 30°CDetection: ELSD(ambient)Sample: 1. L-α-Phosphatidylcholine 2. L-α-Phosphatidylethanolamine 3. L-α-Phosphatidylinositol

App

ID1

5733

Phthalates Column: Luna5μmNH2Dimension: 150x4.6mmPart no.: 00F-4378-E0Mobile Phase: Hexane/Ethanol(95:5)Flow Rate: 1mL/minDetection: UV@254nmSample: 1. DiisooctylPhthalate 2. DibutylPhthalate 3. DiethylPhthalate 4. DimethylPhthalate

App

ID1

4301

Protein Bar Column: Luna5μmNH2Dimension: 250x4.6mmPart no.: 00G-4378-E0Mobile Phase: Acetonitrile/Water(80:20)Flow Rate: 3mL/minTemperature: 40°CDetection: RefractiveIndex(RI)(ambient)Sample: 1. Unknown 2. Fructose 3. Glucose 4. Sucrose 5. Maltose

App

ID1

4293

0 2 4 6 8 min

1

2

4

3

0 2 4 6 10 min

1

2

43

5

8

168 Phenomenex

HPLC

Luna

TM

Luna®

min0

1

2

34

5

6

5 10 15 20 25 30 35 40

Peptides Column: Luna5μmSCXDimensions: 150x4.6mmPart no.: 00F-4398-E0Mobile Phase: A) 20mMPotassiumPhosphate,25%Acetonitrile,pH2.5 B) 20mMPotassiumPhosphate,25%Acetonitrile,400mM PotassiumChloride,pH2.5Gradient: A/B(95:5)toA/B(10:90)in45minutesFlow Rate: 1mL/minTemperature: 35°CDetection: UV@215nmInjection Volume: 2µL(5µgoncolumn)Sample: PeptideMixture-SubstanceP 1. Fragment5-11(+1) 2. Fragment4-11(+1) 3. Fragment2-11(+2) 4. Fragment1-9(+3) 5. Intact(+3) 6. (ARG-PHE-TRP-LEU)(+3)

App

ID1

4765

LUnA SCX (StrOng CAtiOn EXChAngE)Luna SCX provides excellent resolution and peak shape of basic, cationic compounds. However, most SCX columns show poor peak shape and bad resolution causing many chromatographers to ignore this important phase for small molecule method development, until now. Luna SCX contains a benzene sulfonic acid ligand providing ion-exchange reversed phase, and aromatic interactions. Such interaction make Luna SCX great as a first dimension of 2D LC applications as well as improved resolution for small molecules. The result:

• resolving power and sharp peak shape to separate complex cationic/basic and nitrogen containing compounds

• 5 and 10 μm columns and bulk media for analytical through preparative separations

• Benzene Sulfonic acid ligand provides mixed-mode interaction improving separation for 2d peptide applications

20 10 min4 86

1

4

3

2

Childrens tylenol Cold Syrup Column: Luna5μmSCXDimension: 150x4.6mmPart no.: 00F-4398-E0Mobile Phase: 50mMKH2PO4,pH2.5/Acetonitrile(35:65)Injection Volume: 1µLFlow Rate: 1.5mL/minDetection: UV@210nmSample Prep: Dissolve1partChildrensTylenolColdin10partsMethanolSample: 1. Acetaminophen 2. Pseudoephedrine 3. Dextromethorphan 4. Chlorpheniramine

App

ID1

4555

min0 5 10 15 20 25 30 35 40 45

tryptic digest of Bovine Cytochrome c Column: Luna5μmSCXDimensions: 150x4.6mmPart no.: 00F-4398-E0Mobile Phase: A) 20mMPotassiumPhosphate,pH2.5/ 25%Acetonitrile B) 20mMPotassiumPhosphate,pH2.5/ 25%Acetonitrile/350mMPotassiumChlorideGradient: 100%Ato100%Bin50minutesFlow Rate: 1mL/minTemperature: 35°CDetection: UV@215nmInjection Volume: 50µL(20µgoncolumn)Sample: BovineCytochromectrypsindigest

App

ID1

4767

SCX Method Development and pH: ThestandardoperatingpHrangeforLunaSCXis2.0to7.0.MostSCXmethodsaretypicallyrunbetweenpH2.0and5.0foroptimalperformance.Thisensuresthatnitrogen-containinganalytes,especiallythosewithadjacentconjugatedsystemareprotonated.Runninginhighlyacidic(pH<2.0)orbasic(pH>7.0)mobilephasesmaycausethisphasetoundergodegradation,asiscommonforallsilica-basedSCXphases.

see p. 379 for testing probes.2.0-7.03µmand10µmBenzeneSulfonicAcid,StrongCationExchangeAmineandpolyaminecontainingcompoundsGuaranteedtoprovidesharperpeakshapeandbetterresolutioncomparedtotraditionalSCXcolumns


PhaseApplication

Strength

Low High

Hydrophobicity

H-Bonding

AromaticSelectivity


uSP: L9

Luna SCXn/A

n/A

169Phenomenex

HPLCLuna

TM

Luna®

Luna SCX (cont'd)

12 min12 min1010882200

200200

100100

00

mAUmAU

6644

11

66

55

44

33

22

10 12 min820

80

60

40

20

0

mAU

64

1

4, 5

3, 6

2

10 min820

80

60

40

20

100

0

mAU

64

1

6

5

4

3

2

10 12 min820

200

100

0

mAU

64

1, 2

6

5

4

3

App

ID1

4549

App

ID1

4548

App

ID1

4550

App

ID1

4547 Luna® 5 μm

SCXWaters® Spherisorb®

5 μm SCX

Macherey-Nagel Nucleosil® 5 μm SA

Whatman® Partisil® 5 μm SCX

SCX Column Comparison**

** Thecomparativedatapresentedheremaynotberepresentativeforallapplications.

Cocaine and Metabolites Conditionsforallcolumns:Columns: Luna5μmSCX100Å Spherisorb5μmSCX80Å Nucleosil5μmSA100Å Partisil5μmSCX85ÅDimensions: 150x4.6mmMobile Phase: 50mMKH2PO4,pH2.5 /Acetonitrile(65:35)Injection Volume: 20µLFlow Rate: 2.0mL/minDetection: UV@210nm

Sample: 1. m-HydroxylbenzoylEcgonine(80µL/mL) 2. NorbenzoylEcgonine(50µL/mL) 3. Norcocaine(100µL/mL) 4. BenzoylEcgonineIsopropylEster(50µL/mL) 5. Cocaine(85µL/mL) 6. EcgonineMethylEster(690µL/mL)

170 Phenomenex

HPLC

Luna

TM

Luna®

Particle Size

Surface Coverage

Carbon Load %

Particle Size Distribution

Metal (ppm/10)Surface Area (x100m2/g)

Pore Size (x100Å)

Inert test

SRM-869

Selectivity of Basic Drugs,

K/K2

Selectivity of Basic Drugs, K2/K1

Hydrophobicity

Hydrogen Bonding

Excellent reproducibilityIt is tough to meet Luna quality standards. The Silica Reproducibility and Bonded Phase Reproducibility Tanaka plots below illustrate the reproducibility of 13 different quality control parameters for 9 batches of Luna material - nearly no variation is observed. Each batch represents hours of quality control testing for over 25 different parameters. We know chromatographers rely on Luna for rugged, reproducible methods worldwide; therefore, an extensive Materials Validation Document (MVD) accompanying each column confirms consistent column specifications and consistent performance.

Material Validation documentQuality Proven

Extensive testing and quality control test data is shown on the Materials Validation Document (MVD) that accompanies each 3 and 5 μm Luna column. Over 25 individual chemical and physical tests are performed and documented on the MVD, including:

• Particle Analysis

- ParticleSize

- PoreDiameter

- ParticleSizeDistribution

- SurfaceArea

• total Metal Content

• Bonded Phase Coverage

- TotalCarbon

- SurfaceCoverage

• diagnostic Chromatography test

- Inertness

- MetalSensitivity

- HydrophobicIndex

• Performance Chromatography test

- BasicDrugStandards

- LongevityTests(pHStability)

- ScanningElectronMicroscopy(measures surfacesmoothnessandparticleshape)

Column-to-Column reproducibility

Bonded Phase reproducibility

Bonding reproducibility test data from 9 Luna 5 μm C18(2) batches. Since differences in bonded phase coverage can show selectivity changes, each Luna batch undergoes 6 tests for bonding.

Silica reproducibility

Quality control test data designed to monitor subtle changes in Luna silica that may affect reproducibility.

Batch No.

0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

4.00

4.50

5.00

Hydrogen Bonding

Hydrophobic Index

Metal Test

Inertness

Luna 10 µm C18(2) Batch Number

Luna

10

µm C

18(2

) Sp

eci�

catio

n R

ange

s

5293-1

5293-3

5293-5

5293-7

5293-9

5293-11

5293-13

5293-15

5293-17

5293-19

5293-21

5293-23

5293-25

5293-27

5293-29

5293-31

5293-33

5293-35

5293-37

5293-395293-40

With over 10 years of proven reproducibility, you can be confident in your choice to develop methods on Luna. The following graph shows consistency in both inertness and hydrophobicity across 40 batches of Luna 10 µm C18(2).

171Phenomenex

HPLCLuna

TM

Luna®

MercuryMS™ LC/MS Cartridges (mm)

10 x 2.0 10 x 4.0 20 x 2.0 20 x 4.0Qty Pricing 3 μm Phase I I I I LUNA C18(2) 00N-4251-B0-CE 00N-4251-D0-CE 00M-4251-B0-CE 00M-4251-D0-CE

LUNA C8(2) 00N-4248-B0-CE 00N-4248-D0-CE 00M-4248-B0-CE 00M-4248-D0-CE

5 μmLUNA C18(2) 00N-4252-B0-CE 00N-4252-D0-CE 00M-4252-B0-CE 00M-4252-D0-CE

LUNA C8(2) 00N-4249-B0-CE 00N-4249-D0-CE 00M-4249-B0-CE 00M-4249-D0-CE

MercuryMSCartridgesrequireholder,seep.276

3 μm and 5 μm Capillary Columns (mm) 50 x 0.30 150 x 0.30 250 x 0.30 50 x 0.50 150 x 0.50 250 x 0.50 Phases

3μmC8(2) I 00B-4248-AC I 00F-4248-AC I 00G-4248-AC I 00B-4248-AF I 00F-4248-AF I 00G-4248-AF

3μmC18(2) 00B-4251-AC 00F-4251-AC 00G-4251-AC — 00F-4251-AF 00G-4251-AF

5μmC8(2) 00B-4249-AC 00F-4249-AC 00G-4249-AC 00B-4249-AF 00F-4249-AF 00G-4249-AF

5μmC18(2) 00B-4252-AC 00F-4252-AC 00G-4252-AC 00B-4252-AF 00F-4252-AF 00G-4252-AF

OrdEring infOrMAtiOn

3 μm Microbore and Minibore Columns (mm) SecurityGuard™Cartridges 30 x 1.0 50 x 1.0 150 x 1.0 30 x 2.0 50 x 2.0 100 x 2.0 150 x 2.0 4 x 2.0 mm Phases

Silica(2) I — I — I — I 00A-4162-B0 I 00B-4162-B0 I 00D-4162-B0 I 00F-4162-B0 AJ0-4347

C8(2) 00A-4248-A0 00B-4248-A0 00F-4248-A0 00A-4248-B0 00B-4248-B0 00D-4248-B0 00F-4248-B0 AJ0-4289

C18(2) 00A-4251-A0 00B-4251-A0 00F-4251-A0 00A-4251-B0 00B-4251-B0 00D-4251-B0 00F-4251-B0 AJ0-4286

CN — — — 00A-4254-B0 00B-4254-B0 00D-4254-B0 00F-4254-B0 AJ0-4304

Phenyl-Hexyl 00A-4256-A0 00B-4256-A0 — 00A-4256-B0 00B-4256-B0 00D-4256-B0 00F-4256-B0 AJ0-4350

NH2 — — 00F-4377-A0 00A-4377-B0 00B-4377-B0 00D-4377-B0 00F-4377-B0 AJ0-4301

SecurityGuard™AnalyticalCartridgesrequireuniversalholderPartNo.:KJO-4282

forID:2.0-3.0mm

forID:2.0-3.0mm 3.2-8.0mm

Seep.282forFusedSilicaCapillaryAdapterandCapillaryGuardColumns.

3 μm Analytical Columns (mm) SecurityGuard™Cartridges 30 x 3.0 50 x 3.0 150 x 3.0 30 x 4.6 50 x 4.6 75 x 4.6 100 x 4.6 150 x 4.6 4 x 2.0 mm 4 x 3.0 mm Phases

Silica(2) I — I — I 00F-4162-Y0 I — I 00B-4162-E0 I — I 00D-4162-E0 I 00F-4162-E0 AJ0-4347 I AJ0-4348

C8(2) 00A-4248-Y0 00B-4248-Y0 00F-4248-Y0 00A-4248-E0 00B-4248-E0 00C-4248-E0 00D-4248-E0 00F-4248-E0 AJ0-4289 AJ0-4290

C18(2) 00A-4251-Y0 00B-4251-Y0 00F-4251-Y0 00A-4251-E0 00B-4251-E0 00C-4251-E0 00D-4251-E0 00F-4251-E0 AJ0-4286 AJ0-4287

CN — 00B-4254-Y0 00F-4254-Y0 00A-4254-E0 00B-4254-E0 00C-4254-E0 00D-4254-E0 00F-4254-E0 AJ0-4304 AJ0-4305

Phenyl-Hexyl — 00B-4256-Y0 00F-4256-Y0 00A-4256-E0 00B-4256-E0 00C-4256-E0 00D-4256-E0 00F-4256-E0 AJ0-4350 AJ0-4351

NH2 — 00B-4377-Y0 00F-4377-Y0 00A-4377-E0 00B-4377-E0 — 00D-4377-E0 00F-4377-E0 AJ0-4301 AJ0-4302

2.5 μm High Speed Technology (HST) Columns (mm) 30 x 2.0 50 x 2.0 100 x 2.0 50 x 3.0 100 x 3.0 Phase

Luna2.5µmC18(2)-HST I 00A-4446-B0 I 00B-4446-B0 I 00D-4446-B0 I 00B-4446-Y0 I 00D-4446-Y0

Seep.277forinformationaboutHSTColumns.

If Luna does not provide at least anequivalentseparationascomparedtoacompeting columnof the sameparticlesize,similarphaseanddimensions,sendinyourcomparativedatawithin45daysandkeeptheLunacolumnforFREE.

/10pk

/10pk /10pk

172 Phenomenex

HPLC

Luna

TM

Luna®

SecurityGuard™AnalyticalCartridgesrequireuniversalholderPartNo.:KJO-4282

forID:2.0-3.0mm

forID:2.0-3.0mm 3.2-8.0mm

5 μm Microbore and Minibore Columns (mm) SecurityGuard™Cartridges 50 x 1.0 150 x 1.0 250 x 1.0 30 x 2.0 50 x 2.0 150 x 2.0 250 x 2.0 4 x 2.0 mm

Phases

Silica(2) I — I — I — I — I 00B-4274-B0 I 00F-4274-B0 I 00G-4274-B0 AJ0-4347

C5 — — — 00A-4043-B0 00B-4043-B0 00F-4043-B0 — AJ0-4292

C8 — — — 00A-4040-B0 00B-4040-B0 00F-4040-B0 — AJ0-4289

C8(2) 00B-4249-A0 00F-4249-A0 00G-4249-A0 00A-4249-B0 00B-4249-B0 00F-4249-B0 00G-4249-B0 AJ0-4289

C18 — — — — 00A-4041-B0 00B-4041-B0 00F-4041-B0 00G-4041-B0 AJ0-4286

C18(2) 00B-4252-A0 00F-4252-A0 00G-4252-A0 00A-4252-B0 00B-4252-B0 00F-4252-B0 00G-4252-B0 AJ0-4286

CN — — — 00A-4255-B0 00B-4255-B0 00F-4255-B0 00G-4255-B0 AJ0-4304

Phenyl-Hexyl 00B-4257-A0 00F-4257-A0 00G-4257-A0 00A-4257-B0 00B-4257-B0 00F-4257-B0 00G-4257-B0 AJ0-4350

NH2 — 00F-4378-A0 — 00A-4378-B0 00B-4378-B0 00F-4378-B0 00G-4378-B0 AJ0-4301

5 μm Analytical Columns (mm) SecurityGuard™Cartridges 30 x 3.0 50 x 3.0 150 x 3.0 250 x 3.0 30 x 4.6 50 x 4.6 75 x 4.6 4 x 2.0 mm 4 x 3.0 mm Phases

Silica(2) I — I — I — I — I — I 00B-4274-E0 I — AJ0-4347 I AJ0-4348

C5 — — 00F-4043-Y0 — — 00B-4043-E0 — AJ0-4292 AJ0-4293

C8 — — 00F-4040-Y0 00G-4040-Y0 00A-4040-E0 00B-4040-E0 00C-4040-E0 AJ0-4289 AJ0-4290

C8(2) — 00B-4249-Y0 00F-4249-Y0 00G-4249-Y0 00A-4249-E0 00B-4249-E0 00C-4249-E0 AJ0-4289 AJ0-4290

C18 — — 00F-4041-Y0 00G-4041-Y0 00A-4041-E0 00B-4041-E0 00C-4041-E0 AJ0-4286 AJ0-4287

C18(2) 00A-4252-Y0 00B-4252-Y0 00F-4252-Y0 00G-4252-Y0 00A-4252-E0 00B-4252-E0 00C-4252-E0 AJ0-4286 AJ0-4287

CN — 00B-4255-Y0 00F-4255-Y0 00G-4255-Y0 00A-4255-E0 00B-4255-E0 00C-4255-E0 AJ0-4304 AJ0-4305

Phenyl-Hexyl 00A-4257-Y0 00B-4257-Y0 00F-4257-Y0 00G-4257-Y0 00A-4257-E0 00B-4257-E0 00C-4257-E0 AJ0-4350 AJ0-4351

NH2 — 00B-4378-Y0 00F-4378-Y0 00G-4378-Y0 00A-4378-E0 00B-4378-E0 00C-4378-E0 AJ0-4301 AJ0-4302

SCX — — — — — 00B-4398-E0 — AJ0-4307 AJ0-4308


/10pk

/10pk /10pk

173Phenomenex

HPLCLuna

Luna®

TM

forID:3.2-8.0mm 9-16mm

OrdEring infOrMAtiOnSecurityGuard™AnalyticalCartridgesrequireuniversalholderPartNo.:KJO-4282

Seep.218forSecurityGuardCartridgeHolders

5 μm Analytical, Semi-Prep and Preparative Columns (mm) SecurityGuard™ Cartridges 100 x 4.6 150 x 4.6 250 x 4.6 250 x 10 250 x 15 250 x 21.2 250 x 30 4 x 3.0 mm 10 x 10 mm 15 x 21.2 mm Phases

Silica(2) I 00D-4274-E0 I 00F-4274-E0 I 00G-4274-E0 I 00G-4274-N0I — I 00G-4274-P0 I 00G-4274-U0 AJ0-4348 I AJ0-7223 I AJ0-7229

C5 00D-4043-E0 00F-4043-E0 00G-4043-E0 — — — — AJ0-4293 AJO-7372 —

C8 00D-4040-E0 00F-4040-E0 00G-4040-E0 00G-4040-N0 — — — AJ0-4290 AJ0-7222 AJ0-7840

C8(2) 00D-4249-E0 00F-4249-E0 00G-4249-E0 00G-4249-N0 00G-4249-AK 00G-4249-P0 00G-4249-UO AJ0-4290 AJ0-7222 AJ0-7840

C18 00D-4041-E0 00F-4041-E0 00G-4041-E0 00G-4041-N0 — — — AJ0-4287 AJ0-7221 AJ0-7839

C18(2) 00D-4252-E0 00F-4252-E0 00G-4252-E0 00G-4252-N0 00G-4252-AK 00G-4252-P0 00G-4252-U0 AJ0-4287 AJ0-7221 AJ0-7839

CN 00D-4255-E0 00F-4255-E0 00G-4255-E0 00G-4255-N0 — — — AJ0-4305 AJ0-7313 AJ0-8220

Phenyl-Hexyl 00D-4257-E0 00F-4257-E0 00G-4257-E0 00G-4257-N0 — 00G-4257-P0 00G-4257-U0 AJ0-4351 AJ0-7314 AJ0-7841

NH2 00D-4378-E0 00F-4378-E0 00G-4378-E0 00G-4378-N0 — 00G-4378-P0 00G-4378-U0 AJ0-4302 AJ0-7364 AJ0-8162

SCX 00D-4398-E0 00F-4398-E0 00G-4398-E0 00G-4398-N0 — 00G-4398-P0 — AJ0-4308 AJ0-7369 —

15 μm Preparative and Pilot Scale Columns (mm) Guards 250 x 4.6 250 x 21.2 250 x 30 250 x 50 50 x 50 Phases

Silica(2) I 00G-4271-E0 I 00G-4271-P0 I 00G-4271-U0 I 00G-4271-V0 03B-4271-V0

C8(2) 00G-4272-E0 00G-4272-P0 00G-4272-U0 00G-4272-V0 03B-4272-V0

C18(2) 00G-4273-E0 00G-4273-P0 00G-4273-U0 00G-4273-V0 03B-4273-V0

Phenyl-Hexyl 00G-4286-E0 00G-4286-P0 00G-4286-U0 00G-4286-V0 03B-4286-V0

10 μm Semi-Prep and Preparative Columns (mm) Guards SecurityGuard™Cartridges 250 x 4.6 250 x 10 250 x 15 250 x 21.2 250 x 30 250 x 50 50 x 50 10 x 10 mm 15 x 21.2 mm Phases

Silica(2) I 00G-4091-E0 I 00G-4091-N0 I — I 00G-4091-P0 I — I 00G-4091-V0 03B-4091-V0 AJO-7223 I AJO-7229

C5 00G-4092-E0 00G-4092-N0 — 00G-4092-P0 — — — AJO-7372 —

C8 00G-4093-E0 00G-4093-N0 — — — — — AJO-7222 AJO-7840

C8(2) 00G-4250-E0 00G-4250-N0 — 00G-4250-P0 00G-4250-U0 00G-4250-V0 03B-4250-V0 AJO-7222 AJO-7840

C18 00G-4094-E0 00G-4094-N0 — — — — — AJO-7221 AJO-7839

C18(2) 00G-4253-E0 00G-4253-N0 00G-4253-AK 00G-4253-P0 00G-4253-U0 00G-4253-V0 03B-4253-V0 AJO-7221 AJO-7839

CN 00G-4300-E0 00G-4300-N0 — — — 00G-4300-V0 03B-4300-V0 AJO-7313 AJO-8220

Phenyl-Hexyl 00G-4285-E0 00G-4285-N0 — 00G-4285-P0 00G-4285-U0 00G-4285-V0 03B-4285-V0 AJ0-7314 AJO-7841

NH2 00G-4379-E0 00G-4379-N0 — 00G-4379-P0 — — — AJ0-7364 AJO-8162

SCX 00G-4401-E0 00G-4401-N0 — 00G-4401-P0 — — — AJ0-7369 —

forID:9-16mm 18-30mm

ImproveanalytesensitivityandreducebaselinenoisewithStrataSPEtubesandwellplates.Seepp.3-22formoreinformation

Seeourlatestdevelopmentsinhigh-throughoutpurificationsstartingonpage284

18-30mm

* **‡

**‡

*SecurityGuard™ Analytical Cartridges require holder, Part No.: KJ0-4282‡Semi-prep SecurityGuard™ Cartridges require holder, Part No.: AJ0-7220

**PREP SecurityGuard™ Cartridges require holder, Part No.: AJ0-8223

/10pk /3pk /ea

/3pk /ea

174 Phenomenex

HPLC

Luna

TM

Luna®

MEthOd dEVELOPMEnt KitSLuna Method Development Kits combine the excellent performance of Luna HPLC columns with either 3 or 5 different selectivities. The column kits include a Luna Phenyl-Hexyl (moderate polarity) and your choice of a Luna CN (polar) or a C8(2) or C18(2) (non-polars).

MEthOd VALidAtiOn KitSThree columns of the same phase from three different batches provide an excellent benchmark for method validation. Method reproducibility is easily and quickly assessed, clearly demonstrating chromatographic performance validation potential and long term compliance with Luna Method Validation Kits.


Method Development KitsPart no. Description Dimensions (mm) unit Price

KH0-4760 I LunaMethodDevelopmentKit(5columns) I

I I 1eachof5μmC18(2),C8(2),C5,CN,Phenyl-Hexylphases 150x4.6 5/pk

KH0-4354 LunaRapid-MDKit(3columns) 1eachof3μmC18(2),C8(2)andPhenyl-Hexylphases 50x4.6 3/pk

KHO-4761 LunaSelectivityKit1(3columns) 1eachof5μmC18(2),Phenyl-Hexyl,CNphases 150x4.6 3/pk

KH0-4762 LunaSelectivityKit2(3columns) 1eachof5μmC8(2),Phenyl-Hexyl,CNphases 150x4.6 3/pk

Method Validation KitsPart no. Description Dimensions (mm)* unit Price

KH0-4356 I Luna5μmC18(2)MethodValidationKit I 150x4.6 I 3/pk I

KH0-4358 Luna5μmC8(2)MethodValidationKit 150x4.6 3/pk

KH0-4359 Luna5μmPhenyl-HexylMethodValidationKit 150x4.6 3/pk

*Otherdimensionsavailableonrequest.

Seep.89forGeneralPurpose/PharmaceuticalMethodDevelopmentKits.

175Phenomenex

HPLCLuna

TM

Luna®

BULK MAtEriALOrdEring infOrMAtiOn

10 μm Bulk Packings 100 g 1 kg 5 kg 10 kg 50 kg 100 kg Phases

Silica(2) I 04G-4091 I 04K-4091 I 04L-4091 I 04M-4091 I 04N-4091 I 04P-4091

C8(2) 04G-4250 04K-4250 04L-4250 04M-4250 04N-4250 04P-4250

C18(2) 04G-4253 04K-4253 04L-4253 04M-4253 04N-4253 04P-4253

CN 04G-4300 04K-4300 04L-4300 04M-4300 04N-4300 04P-4300

NH2 04G-4379 04K-4379 04L-4379 04M-4379 04N-4379 04P-4379

SCX 04G-4401 04K-4401 04L-4401 04M-4401 04N-4401 04P-4401

Phenyl-Hexyl 04G-4285 04K-4285 04L-4285 04M-4285 04N-4285 04P-4285

15 μm Bulk Packings 100 g 1 kg 5 kg 10 kg 50 kg 100 kg Phases


C8(2) 04G-4272 04K-4272 04L-4272 04M-4272 04N-4272 04P-4272

C18(2) 04G-4273 04K-4273 04L-4273 04M-4273 04N-4273 04P-4273


10 μm-PREP Bulk Packings

LowercostexcellentperformancealternativetoLUNA10μm.

100 g 1 kg 5 kg 10 kg 50 kg 100 kg Phases


C8(2) 04G-4323 04K-4323 04L-4323 04M-4323 04N-4323 04P-4323

C18(2) 04G-4324 04K-4324 04L-4324 04M-4324 04N-4324 04P-4324


If Luna does not provide at least anequivalentseparationascomparedtoacompeting columnof the sameparticlesize,similarphaseanddimensions,sendinyourcomparativedatawithin45daysandkeeptheLunacolumnforFREE.

develop or improve your hPLC Method - Brechbühler AG · Can be used as reversed- or normal-phase...

Documents

Transcript of develop or improve your hPLC Method - Brechbühler AG · Can be used as reversed- or normal-phase...