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DAPI Flag-FOXO3a Merged WT MEFs Untreated Doxorubicin (16h) Untreated Doxorubicin (16h) p38α -/- MEFs Supplementary Figure S7. The transfected Flag-FOXO3a accumulates in the nuclei of the wild-type but not p38α-deficent MEFs following doxorubicin treatment Wild-type and p38α-deficent MEFs were seeded into slide chamber, transfected with the pCMV-Flag-FOXO3a (WT) and incubated with media in the absence or presence of doxorubicin for 16 h. Immunofluorescent staining was then performed using the Flag antibodies and DAPI. All images shown are typical results obtained from at least 10 different fields.

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Flag-FOXO3a. DAPI. Merged. Untreated. WT MEFs. Doxorubicin (16h ). Untreated. p38α -/- MEFs. Doxorubicin (16h). Supplementary Figure S7. The transfected Flag-FOXO3a accumulates in the nuclei of the wild-type but not p38α-deficent MEFs following doxorubicin treatment - PowerPoint PPT Presentation

Transcript of DAPI

Page 1: DAPI

DAPIFlag-FOXO3a Merged

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MEF

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Untreated

Doxorubicin (16h)

Untreated

Doxorubicin (16h)

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-/- M

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Supplementary Figure S7. The transfected Flag-FOXO3a accumulates in the nuclei of the wild-type but not p38α-deficent MEFs following doxorubicin treatmentWild-type and p38α-deficent MEFs were seeded into slide chamber, transfected with the pCMV-Flag-FOXO3a (WT) and incubated with media in the absence or presence of doxorubicin for 16 h. Immunofluorescent staining was then performed using the Flag antibodies and DAPI. All images shown are typical results obtained from at least 10 different fields.

Introduction to Cell & Molecular Biology Techniques ... · Introduction to Cell & Molecular Biology Techniques : Immunofluorescence DAPI (DNA) GFP γ Tubulin Merge

Introduction to Cell & Molecular Biology Techniques ... · Introduction to Cell & Molecular Biology Techniques : Immunofluorescence DAPI (DNA) GFP γ Tubulin Merge

DAPI H2AX RRM1 DAPI Cont siRNA RRM1 - Genes & Developmentgenesdev.cshlp.org/content/suppl/2010/01/25/24.4.333.DC1/... · 2010. 1. 25. · B C TFL TN1 TN2 TC2 TC1 CHROMO Binding RRM1

DAPI H2AX RRM1 DAPI Cont siRNA RRM1 - Genes & Developmentgenesdev.cshlp.org/content/suppl/2010/01/25/24.4.333.DC1/... · 2010. 1. 25. · B C TFL TN1 TN2 TC2 TC1 CHROMO Binding RRM1

Supplementary Figure 1-8 - images.nature.com · VZ 100μm WT Disc1 LI 0 100 200 300 400 Number of BrdU- positive cells WT Disc1 LI BrdU BrdU / DAPI Supplementary Figure 1 SVZ VZ a

Supplementary Figure 1-8 - images.nature.com · VZ 100μm WT Disc1 LI 0 100 200 300 400 Number of BrdU- positive cells WT Disc1 LI BrdU BrdU / DAPI Supplementary Figure 1 SVZ VZ a

-HA B A C1 A HA A RPP F - media.nature.com fileα-HA α-Kcr MERGE DAPI α-HA α-Kac MERGE DAPI F-RPP F F A A D HA-C1 HA-C1 A A A A A B Supplementary Figure S7. Various HDAC1 mutations

-HA B A C1 A HA A RPP F - media.nature.com fileα-HA α-Kcr MERGE DAPI α-HA α-Kac MERGE DAPI F-RPP F F A A D HA-C1 HA-C1 A A A A A B Supplementary Figure S7. Various HDAC1 mutations

NIH Public Access Saravanan Ayyadurai Emilie Viennois Moiz ...RNA interference (RNAi) mediated by small interfering RNAs (siRNAs) of 19–23 base ... (DAPI), Stealth RNAiTM siRNA negative

NIH Public Access Saravanan Ayyadurai Emilie Viennois Moiz ...RNA interference (RNAi) mediated by small interfering RNAs (siRNAs) of 19–23 base ... (DAPI), Stealth RNAiTM siRNA negative

DEPARTMENT OF BIOPATHOLOGY AND THERAPY OF … · 2019. 10. 7. · phalloidin labelled F-actin (red) and DAPI nuclear staining (blue) – left side. Hydrogel formula supports cell

DEPARTMENT OF BIOPATHOLOGY AND THERAPY OF … · 2019. 10. 7. · phalloidin labelled F-actin (red) and DAPI nuclear staining (blue) – left side. Hydrogel formula supports cell