Ch 14: TLC & Accessories - HPLC-Säulen, GC-Säulen ... · PDF filecommon TLC sorbent....
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TLC & ACCESSORIES For all of your analytical needs, visit us at sigma-aldrich.com/analytical Thin Layer Chromatography 2 HPTLC Plates 2 Standard Analytical TLC Plates 4 TLC Plates - Glass Support ....................................... 4 TLC Plates - Aluminum Foil Support ............................... 7 TLC Plates - Polyester (PET) Support ............................... 8 Prep TLC Plates 8 TLC Accessories 9 TLC Dessicating Cabinets ....................................... 9 TLC Developing Tanks & Chambers ............................... 9 Spectroline UV Viewing Cabinets ................................ 10 UV Lamps & Accessories ....................................... 10 TLC Plate Racks .............................................. 11 Adsorbent Scrapers ........................................... 12 Cutting Tools ................................................ 12 Syringes & Pipettes ........................................... 12 Other TLC Accessories ......................................... 12 Bulk TLC Adsorbents 13 Bulk Silica for TLC with Fluorescence ............................. 13 Bulk Silica for TLC without Flurorescence .......................... 13 Bulk Cellulose for TLC ......................................... 13 Bulk Polyamide for TLC ........................................ 13 Bulk FLORISIL for TLC .......................................... 13 Sequential Spraying Makes Drug and Lipid Detection Simpler 14 TLC Sprayers 14 TLC Reagents 16
Transcript of Ch 14: TLC & Accessories - HPLC-Säulen, GC-Säulen ... · PDF filecommon TLC sorbent....
TLC & ACCESSORIES
For all of your analytical needs, visit us at sigma-aldrich.com/analytical
Thin Layer Chromatography 2
HPTLC Plates 2
Standard Analytical TLC Plates 4TLC Plates - Glass Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4TLC Plates - Aluminum Foil Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7TLC Plates - Polyester (PET) Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Prep TLC Plates 8
TLC Accessories 9TLC Dessicating Cabinets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9TLC Developing Tanks & Chambers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9Spectroline UV Viewing Cabinets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10UV Lamps & Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10TLC Plate Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11Adsorbent Scrapers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12Cutting Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12Syringes & Pipettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12Other TLC Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Bulk TLC Adsorbents 13Bulk Silica for TLC with Fluorescence . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13Bulk Silica for TLC without Flurorescence . . . . . . . . . . . . . . . . . . . . . . . . . . 13Bulk Cellulose for TLC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13Bulk Polyamide for TLC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13Bulk FLORISIL for TLC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Sequential Spraying Makes Drug and LipidDetection Simpler 14
TLC Sprayers 14
TLC Reagents 16
Table of Contents
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Thin Layer ChromatographyEnsure Selection, Quality and Performance
BrandsWe offer our own high quality brand of TLC plates as well as brand nameTLC plates from Merck* and Analtech to meet our customers’ needs forbrand name, quality and price.
Plate TypesWe have a range of high performance TLC (HPTLC) plates as well as anextensive offering of standard analytical and preparative TLC plates.
SupportsThree different types of supports (or backings) are available to meet yourindividual application requirements.
• Glass• Aluminum Foil• Plastic (Polyester – PET)
Adsorbent Layers/MatricesOur glass, aluminum foil and PET (polyester) TLC plates are available coatedwith a variety of matrices and are available with and without fluorescentindicator.Available matrices include:
• Silica gel (unmodified, modified/bonded, chiral and high purity) – mostcommon TLC sorbent. Eluents used are similar to those used for normalphase HPLC. Does not have the catalytic properties of alumina.
• Aluminum oxide – exhibits selectivity similar to, although slightly differentthan, silica. Second most common TLC sorbent.
• Cellulose – available as either microcrystalline or fibrous cellulose. Spotsare generally more compact when separated on layers of microcrystallinecellulose than when separated on layers of fibrous cellulose.
• Polyamide – exhibits low diffusivity, allowing for tightly confined samplespots with maximum intensity. Corrosive visualization reagents are notrecommended.
BindersPlates are available with polymeric (organic) and inorganic binders as well aswithout binders. Binders do not generally impact the adsorptive propertiesof the matrix, instead they impact the adherence of the adsorbent layer tothe backing.Available binders:
• Polymeric (organic) binders - Plates with adsorbent layers containingpolymeric binders are generally most rugged, making sample handlingand application easier. They also permit the use of higher water content inthe developing solvent. They are generally recommended for all TLCapplications except for those that use charring for visualization.
• Inorganic binder - Plates with adsorbent layers containing inorganicbinders are not as rugged as those with organic binders but are generallymore rugged than plates without a binder or with a gypsum binder. Theyare the most water resistant and are often used for preparative TLC platesbecause thay are soft, allowing for spots containing the target molecule tobe easily scraped off the support for elution and recovery. Plates withinorganic binder are recommended for applications requiring charring forvisualization, or when developing or visualization reagents interact with anorgainc binder.
• Gypsum or no binder - Plates with adsorbent layers that do not contain abinder or that use a gypsum binder are generally least rugged andfracture easily. They are not recommended for use with developingsolvents containing more that 20% water. Like plates with inorganic layers,they allow spots containing target molecules to be easily scraped off thesupport for elution and recovery and are recommended for applicationsrequiring charring for visulization.
Plate DimensionsChoose from a comprehensive variety of plate dimensions:
• Larger plates (such as 20 cm x 20 cm plates) are oftern less costly as theycan be cut into smaller sizes as needed.
• Pre-scoring (available on some of our glass plates) makes cutting glassplates easier and safer as they can be easily snapped into smallerdimensions.
• Aluminum and plastic (PET)-backed plates are easy to cut to desireddimensionis with a pair of scissors.
• Smaller, pre-cut plates are also available and offer the advantages ofconvenience and ease of use that may make them the more practicalchoice in lavbs where productivity rather than per plate cost is the majordriver.
HPTLC PlatesAchieve Faster Separations and Increased SensitivityMuch like HPLC grew out of improvements in column chromatography, HPTLC grew out of improvements in the quality of sorbents, consistency of platemanufacture, techniques and equipment for sample and detection reagent application, and a generally greater understanding of chromatographic theory.HPTLC (high performance TLC) plates are characterized by smaller particles (≤10 μm), thinner layers (≤150 μm) and smaller plates (≤10 cm developingdistance). In addition, the particle size distribution of the sorbent is typically narrower than for standard TLC layers. The thinner layers allow for faster solventmigration. Sample volumes for HPTLC are generally less than one microliter. The smooth surface of HPTLC plates reduces noise when performingdensitometric quantitation, increasing sensitivity.
HPTLC plates offer users the following advantages over conventional TLC:• Better resolving power per unit distance• Faster development times• Lower sample diffusion• Reduced solvent consumption
Like our standard analytical TLC plates, our HPTLC plates are available coated with a variety of matrices, with and without indicators.
TLC & Accessories
Thin Layer Chromatography
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Silica Gel Matrix on Glass for HPTLC
Fluorescent Indicator Layer Thickness (μm) Binder Brand Cat. No. Qty
L × W 10 × 10 cm(scored)
Yes 150 Inorganic Analtech Z265330-1PAK 25 ea
L × W 10 × 10 cm
No 150 Organic Analtech Z265292-1PAK 25 ea
No 150 Inorganic Analtech Z265314-1PAK 25 ea
Yes 150 Organic Analtech Z265306-1PAK 25 ea
Yes 150 Inorganic Analtech Z265322-1PAK 25 ea
C18-Silica Gel Matrix on Glass for HPTLC
Fluorescent Indicator Layer Thickness (μm) Binder Brand Cat. No. Qty
L × W 10 × 10 cm
No 150 Organic Analtech Z265365-1PAK 25 ea
Yes 150 Organic Analtech Z265373-1PAK 25 ea
L × W 10 × 10 cm(50% silanized)
Yes 200 Organic Analtech Z265411-1PAK 25 ea
L × W 10 × 10 cm(high % carbon)
No 150 Organic Sigma-Aldrich Z122750 25 ea
C8-Silica Gel Matrix on Glass for HPTLC
Fluorescent Indicator Layer Thickness (μm) Binder Brand Cat. No. Qty
L × W 10 × 10 cm
Yes 150 Organic Analtech Z265403-1PAK 25 ea
Amino-Silica Gel Matrix on Glass for HPTLC
Fluorescent Indicator Layer Thickness (μm) Binder Brand Cat. No. Qty
L × W 10 × 10 cm
Yes 150 Organic Analtech Z265349-1PAK 25 ea
Cyano-Silica Gel Matrix on Glass for HPTLC
Fluorescent Indicator Layer Thickness (μm) Binder Brand Cat. No. Qty
L × W 10 × 10 cm
Yes 150 None Analtech Z265357-1PAK 25 ea
Fibrous Cellulose Matrix on Glass for HPTLC
Fluorescent Indicator Layer Thickness (μm) Binder Brand Cat. No. Qty
L × W 10 × 20 cm
No 100 Proprietary Sigma-Aldrich 95412-50EA 50 ea
L × W 20 × 20 cm
Yes 100 Proprietary Sigma-Aldrich 73128-25EA 25 ea
Nano Silica Gel Matrix on Aluminum Foil for HPTLC
Fluorescent Indicator Layer Thickness (μm) Binder Brand Cat. No. Qty
L × W 20 × 20 cm
Yes 150 Organic Sigma-Aldrich 60761-25EA 25 ea
TLC & Accessories
HPTLC Plates
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Fibrous Cellulose Matrix on Aluminum Foil for HPTLC
Fluorescent Indicator Layer Thickness (μm) Binder Brand Cat. No. Qty
L × W 10 × 20 cm
Yes 100 Proprietary - 22185-20EA-F 20 ea
L × W 20 × 20 cm
No 100 Proprietary Sigma-Aldrich 95413-25EA 25 ea
Cellulose Matrix on Polyester (PET) for HPTLC
Fluorescent Indicator Layer Thickness (μm) Binder Brand Cat. No. Qty
L × W 20 × 20 cm
No 100 None Sigma-Aldrich Z122866-25EA 25 ea
L × W 20 × 20 cm(PEI cellulose)
Yes 100 Polymeric Sigma-Aldrich Z122882-25EA 25 ea
L × W 20 × 20 cm(microcrystalline cellulose)
Yes 100 Proprietary Sigma-Aldrich 95725-25EA 25 ea
Polyamide 6 Matrix on Polyester (PET) for HPTLC
Fluorescent Indicator Layer Thickness (μm) Binder Brand Cat. No. Qty
L × W 20 × 20 cm
No 100 Proprietary - 17288-25EA 25 ea
Yes 100 Proprietary Sigma-Aldrich 17289-25EA 25 ea
Standard Analytical TLC PlatesDevelop the Method and Verify ResultsThin Layer Chromatography can be used for both qualitative and quantitative analysis. Standard analytical TLC plates typically have adsorbent layers that arenominally between 200 and 250 microns in thickness. Our standard TLC plates are available coated with a variety of matrices, with and without indicators.
TLC Plates - Glass Support
Glass is the most common plate backing because it is rigid, transparent and chemically resistant to all mobile phases and visualization methods, most notablyto charring with corrosive agents. Glass plates are also reusable; an important consideration for analysts who make their own plates. For in situ densitometricquantification, the use of highly uniform pre-coated layers on glass is virtually mandatory for adequate reproducibility and accuracy. Scored glass plates arealso available that can be easily snapped into smaller, more convenient sizes to accommodate fewer samples or smaller developing tanks.
High Purity Silica Gel Matrix on Glass
Fluorescent Indicator Layer Thickness (μm) Binder Brand Cat. No. Qty
L × W 5 × 10 cm
No 250 Gypsum/polymeric Sigma-Aldrich 60762-50EA 50 ea
Yes 250 Gypsum/polymeric - 60763-50EA 50 ea
L × W 20 × 20 cm(w/ concentration zone)
Yes 250 Gypsum/polymeric Sigma-Aldrich 60768-25EA 25 ea
L × W 20 × 20 cm(recommended for aflatoxin separation)
Yes 250 Gypsum/polymeric Sigma-Aldrich 08572-25EA-F 25 ea
Silica Gel Matrix on Glass w/o Fluorescent Indicator
Fluorescent Indicator Layer Thickness (μm) Binder Brand Cat. No. Qty
L × W 5 × 20 cm
No 250 Polymeric Sigma-Aldrich Z122688-100EA 100 ea
No 250 Inorganic Analtech Z265608-1PAK 25 ea
No 250 Organic Analtech Z265535-1PAK 25 ea
L × W 10 × 10 cm
No 200 Organic Sigma-Aldrich 09918-25EA 25 ea
TLC & Accessories
HPTLC Plates
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Fluorescent Indicator Layer Thickness (μm) Binder Brand Cat. No. Qty
L × W 10 × 20 cm
No 250 Polymeric Sigma-Aldrich Z185310-50EA 50 ea
No 250 Organic Merck Z292966-1PAK 50 ea
No 250 Inorganic Analtech Z265586-1PAK 25 ea
No 250 Organic Analtech Z265519-1PAK 25 ea
L × W 10 × 20 cm(impregnated with caffeine for PAH analytics (DIN 38407, part 7))
No 200 Proprietary Sigma-Aldrich 60796-50EA 50 ea
L × W 20 × 20 cm
No 250 Polymeric Sigma-Aldrich 99570-25EA99570-1EA
25 ea25 ea
No 250 Organic Merck Z292974-1PAK 25 ea
No 250 Inorganic Analtech Z265551-1PAK 25 ea
No 250 Organic Analtech Z265497-1PAK 25 ea
Silica Gel Matrix on Glass with Fluorescent Indicator
Fluorescent Indicator Layer Thickness (μm) Binder Brand Cat. No. Qty
L × W 2.5 × 7.5 cm
Yes 250 Organic Merck Z292982-1PAK 100 ea
L × W 5 × 10 cm
Yes 250 Organic Merck Z292990-1PAK 200 ea
L × W 5 × 20 cm
Yes 250 Inorganic Analtech Z265616-1PAK 25 ea
Yes 250 Organic Merck Z293008-1PAK 100 ea
Yes 250 Polymeric Sigma-Aldrich 99569-100EA 100 ea
Yes 250 Polymeric Sigma-Aldrich Z122696-100EA 100 ea
Yes 250 Organic Analtech Z265543-1PAK 25 ea
L × W 10 × 10 cm
Yes 250 Polymeric Sigma-Aldrich 99573-1EA99573-25EA
25 ea25 ea
Yes 200 Organic Sigma-Aldrich 09916-1SET09916-25EA
1 ea25 ea
L × W 10 × 20 cm(scored)
Yes 250 Inorganic Analtech Z274283-1PAK 25 ea
L × W 10 × 20 cm
Yes 250 Organic Analtech Z265527-1PAK 25 ea
Yes 200 Organic Sigma-Aldrich 09922-50EA 50 ea
Yes 250 Polymeric Sigma-Aldrich 99876-50EA99876-1EA
50 ea50 ea
Yes 250 Organic Merck Z293016-1PAK 50 ea
L × W 10 × 20 cm(w/ concentration zone)
Yes 250 Proprietary Sigma-Aldrich 60767-50EA 50 ea
L × W 20 × 20 cm
Yes 250 Inorganic Analtech Z265578-1PAK 25 ea
Yes 250 Organic Analtech Z265500-1PAK 25 ea
Yes 250 Organic Merck Z293024-1PAK 25 ea
Yes 250 Polymeric Sigma-Aldrich 99571-25EA 25 ea
Yes 250 Polymeric Sigma-Aldrich Z122726-25EA 25 ea
C18-Silica Gel Matrix on Glass w/o Fluorescent Indicator
Fluorescent Indicator Layer Thickness (μm) Binder Brand Cat. No. Qty
L × W 5 × 20 cm
No 250 Inorganic Analtech Z265470-1PAK 25 ea
L × W 10 × 20 cm
No 250 Inorganic Analtech Z265454-1PAK 25 ea
L × W 20 × 20 cm(scored)
No 250 Inorganic Analtech Z500666-1PAK 25 ea
L × W 20 × 20 cm
No 250 Inorganic Analtech Z265438-1PAK 25 ea
TLC & Accessories
Standard Analytical TLC PlatesTLC Plates - Glass Support
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C18-Silica Gel Matrix on Glass with Fluorescent Indicator
Fluorescent Indicator Layer Thickness (μm) Binder Brand Cat. No. Qty
L × W 5 × 20 cm
Yes 250 None Analtech Z265489-1PAK 25 ea
L × W 10 × 10 cm
Yes 250 Organic Sigma-Aldrich 99575-25EA 25 ea
L × W 10 × 20 cm(scored)
Yes 250 None Analtech Z500992-1PAK 25 ea
L × W 10 × 20 cm
Yes 250 None Analtech Z265462-1PAK 25 ea
L × W 10 × 20 cm(w/ low %C)
Yes 250 Polymeric Sigma-Aldrich Z292923-1PAK 50 ea
L × W 20 × 20 cm(scored)
Yes 250 None Analtech Z500887-1PAK 25 ea
L × W 20 × 20 cm
Yes 250 None Analtech Z265446-1PAK 25 ea
Yes 250 Organic Merck Z293032-1PAK 25 ea
Chiral Silica Gel Matrix on Glass
Fluorescent Indicator Layer Thickness (μm) Binder Brand Cat. No. Qty
L × W 5 × 20 cm
Yes 250 Organic - 99697-50EA 50 ea
L × W 10 × 20 cm
No 250 Polymeric Sigma-Aldrich Z148687-4EAZ148687-25EA
4 ea25 ea
L × W 20 × 20 cm
No 250 Polymeric Sigma-Aldrich Z148717-25EA 25 ea
Fibrous Cellulose Matrix on Glass
Fluorescent Indicator Layer Thickness (μm) Binder Brand Cat. No. Qty
L × W 10 × 20 cm
No 250 Proprietary Sigma-Aldrich Z122815-50EA 50 ea
Yes 250 Proprietary - Z122823-50EA 50 ea
L × W 20 × 20 cm
No 250 Proprietary Sigma-Aldrich Z122831-25EA 25 ea
Yes 250 Proprietary Sigma-Aldrich Z122858-25EA 25 ea
Microcrystalline Cellulose Matrix on Glass
Fluorescent Indicator Layer Thickness (μm) Binder Brand Cat. No. Qty
L × W 5 × 20 cm
Yes 250 None Analtech Z265799-1PAK 25 ea
L × W 20 × 20 cm(scored)
Yes 250 None Analtech Z513083-1PAK 25 ea
L × W 20 × 20 cm
No 250 None Analtech Z265764-1PAK 25 ea
Yes 250 None Analtech Z265772-1PAK 25 ea
Aluminum Oxide Matrix on Glass
Fluorescent Indicator Layer Thickness (μm) Binder Brand Cat. No. Qty
L × W 5 × 20 cm
No 250 Inorganic Analtech Z265659-1PAK 25 ea
Yes 250 Inorganic Analtech Z265667-1PAK 25 ea
L × W 10 × 20 cm(scored)
Yes 250 Inorganic Analtech Z500550-1PAK 25 ea
TLC & Accessories
Standard Analytical TLC PlatesTLC Plates - Glass Support
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Fluorescent Indicator Layer Thickness (μm) Binder Brand Cat. No. Qty
L × W 20 × 20 cm
No 250 Inorganic Analtech Z265632-1PAK 25 ea
Yes 250 Inorganic Analtech Z265640-1PAK 25 ea
No 250 Organic Sigma-Aldrich 02665-25EA 25 ea
Yes 250 Organic Sigma-Aldrich 90066-25EA 25 ea
TLC Plates - Aluminum Foil Support
Plates that employ aluminum foil backing are easy to handle, lightweight and flexible. They can be easily cut to desired dimensions with scissors or stored inlaboratory notebooks. They have high solvent resistance and good heat stability. A strong adsorbent layer adherence also makes them a good choice for usewith eluants containing high concentrations of water. However, unlike glass backing, aluminum foil backing is not reusable and aluminum backed plates arenot as chemically resistant as glass to reagents such as mineral acids and concentrated ammonia.
High Performance Silica on Aluminum Foil
• Outstanding wettability (even for 100 % aqueous visualisation reagents)• Easy and precise cutting (no flaking of silica)• Excellent separation efficiency and reproducibility from batch to batch
Fluorescent Indicator Layer Thickness (μm) Binder Brand Cat. No. Qty
L × W 4 × 8 cm
Yes 200 Polymeric Sigma-Aldrich 49859-50EA 50 ea
L × W 5 × 7.5 cm
Yes 200 Polymeric Sigma-Aldrich 52038-20EA 20 ea
No 200 Polymeric Sigma-Aldrich 55811-20EA 20 ea
L × W 5 × 10 cm
Yes 200 Polymeric Sigma-Aldrich 23478-50EA 50 ea
No 200 Polymeric Sigma-Aldrich 75196-50EA 50 ea
L × W 5 × 20 cm
Yes 200 Polymeric Sigma-Aldrich 12606-50EA 50 ea
No 200 Polymeric Sigma-Aldrich 92572-50EA 50 ea
L × W 20 × 20 cm
Yes 200 Polymeric Sigma-Aldrich 56524-25EA 25 ea
No 200 Polymeric Sigma-Aldrich 53356-25EA 25 ea
Silica Gel Matrix on Aluminum Foil
Fluorescent Indicator Layer Thickness (μm) Binder Brand Cat. No. Qty
L × W 4 × 8 cm
Yes 200 Polymeric Sigma-Aldrich 70643-50EA 50 ea
L × W 5 × 10 cm
Yes 200 Polymeric Sigma-Aldrich Z193275-1PAK 50 ea
No 200 Polymeric Sigma-Aldrich 70644-50EA 50 ea
Yes 200 Polymeric Sigma-Aldrich 91835-50EA 50 ea
L × W 10 × 20 cm
Yes 200 Polymeric Sigma-Aldrich 60800-20EA 20 ea
No 200 Polymeric Sigma-Aldrich 02599-20EA 20 ea
L × W 20 × 20 cm
Yes 200 Polymeric Sigma-Aldrich 60778-25EA 25 ea
No 200 Polymeric Sigma-Aldrich 60805-25EA 25 ea
Yes 200 Polymeric Sigma-Aldrich Z193291-1PAK 25 ea
Aluminum Oxide on Aluminum Foil
Fluorescent Indicator Layer Thickness (μm) Binder Brand Cat. No. Qty
L × W 20 × 20 cm
Yes 200 Organic Sigma-Aldrich 06408-25EA 25 ea
TLC & Accessories
Standard Analytical TLC PlatesTLC Plates - Glass Support
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TLC Plates - Polyester (PET) Support
Plates that utilize PET (polyethylene terephthalate) are easy to handle, lightweight and flexible. Developed PET-plates can be stored in laboratory notebooks fordocumentation or cut into strips; options that are not possible with glass plates. However, their flexibility can cause the sorbent layer to break and care mustbe taken not to allow them to curl during drying and activation processes. However, unlike glass backing, PET backing is not reusable and because PET backedplates have lower heat stability, charring must be done at lower temperatures.
High Purity Silica Gel Matrix on Polyester (PET)
Fluorescent Indicator Layer Thickness (μm) Binder Brand Cat. No. Qty
L × W 20 × 20 cm
Yes 250 Gypsum/polymeric Sigma-Aldrich Z122807-25EA 25 ea
Silica Gel Matrix on Polyester (PET)
Fluorescent Indicator Layer Thickness (μm) Binder Brand Cat. No. Qty
L × W 4 × 8 cm
Yes 250 Proprietary - 89070-50EA 50 ea
L × W 20 × 20 cm
Yes 200 Proprietary Sigma-Aldrich 99577-25EA 25 ea
No 250 Polymeric Sigma-Aldrich Z122777-25EA 25 ea
Yes 200 Polymeric Sigma-Aldrich Z122785-25EA 25 ea
Aluminum Oxide Matrix on Polyester (PET)
Fluorescent Indicator Layer Thickness (μm) Binder Brand Cat. No. Qty
L × W 4 × 8 cm
Yes 200 Organic - 89071-50EA 50 ea
L × W 20 × 20 cm
No 200 Organic Sigma-Aldrich Z234206-1PAK 25 ea
Yes 200 Organic Sigma-Aldrich Z234214-1PAK 25 ea
Prep TLC PlatesIsolate and Recover Your CompoundPreparative TLC is used to purify and isolate a particular substance by separating it from contaminants. Preparative TLC plates have adsorbent layers that aregreater than 500 microns in thickness allowing them to be used for the separation of larger quantities of material. When a binder is required, the softerinorganic binders are often used so the sample bands can be more easily removed. Our glass plates for Preparative TLC are available coated with bothunmodified and bonded silica matrices and are available with and without fluorescent indicator.
Silica Gel Matrix on Glass for Prep TLC
Fluorescent Indicator Layer Thickness (μm) Binder Brand Cat. No. Qty
L × W 20 × 20 cm
No 500 Inorganic Analtech Z512990-1PAK 25 ea
Yes 500 Inorganic Analtech Z513032-1PAK 25 ea
No 1,000 Inorganic Analtech Z265810-1PAK 25 ea
Yes 1,000 Inorganic Analtech Z265829-1PAK 25 ea
No 1,500 Inorganic Analtech Z513016-1PAK 25 ea
Yes 1,500 Inorganic Analtech Z513040-1PAK 25 ea
No 2000 Inorganic Analtech Z513024-1PAK 25 ea
Yes 2,000 Inorganic Analtech Z513059-1PAK 25 ea
C18-Silica Gel Matrix on Glass for Prep TLC
Fluorescent Indicator Layer Thickness (μm) Binder Brand Cat. No. Qty
L × W 20 × 20 cm
Yes 1,000 Inorganic Analtech Z272426-1PAK 25 ea
TLC & Accessories
Standard Analytical TLC PlatesTLC Plates - Polyester (PET) Support
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TLC Accessories
TLC Dessicating Cabinets
TLC desiccating cabinet
provides complete visability of stored TLC plates under desiccatingconditions
Acrylic cabinet provides complete visability of stored TLC plates underdesiccating conditions. Stores up to 24 (20 × 20 cm) plates. Plates should beoven dried before storage for optimal results.
Z266086-1EA 1 ea
TLC Developing Tanks & Chambers
Aldrich® rectangular TLC developing tanks, complete
Tank L × H × W (cm) Cat. No. Qty
7.5 × 15.5 × 8.0 Z204226-1EA 1 ea
12.1 × 10.8 × 8.3 Z146226-1EA 1 ea
17.5 × 6.2 × 6.8 Z204196-1EA 1 ea
17.5 × 11.0 × 6.2 Z204188-1EA 1 ea
17.5 × 16.0 × 6.2 Z204161-1EA 1 ea
17.5 × 16.0 × 8.2 Z204153-1EA 1 ea
27.0 × 26.5 × 7.0 Z126195-1EA 1 ea
Latch-lid™ TLC developing chambers
Latch-Lid™ units have a unique stainless steel latching device that holds thelid and tank firmly in place. This permits complete saturation of the chamberwith solvent vapor for uniform development of chromatograms. Glass tankfeatures flat ground tops, inner and outer rims beveled for safety, and flatground bottoms for stability. Accessorize with multi-plate TLC racks forsimultaneous development of up to six TLC plates.
Racks not included.
Description Cat. No. Qty
For use with 10 cm × 10 cm plates Z266019-1EA 1 ea
For use with 20 cm × 20 cm plates Z266000-1EA 1 ea
Aldrich® TLC developing tank - cylindrical
Can be used as a staining chamber. Made of glass with lid. Compact sizeoffers good plate visibility and minimal solvent use.
Description Cat. No. Qty
glass tank, O.D. 6.5 cm × H 10.5 cm Z243906-1EAZ243906-6X1EA
1 ea6 × 1 ea
glass tank, O.D. 6.5 cm × H 21.0 cm Z243914-1EAZ243914-3EA
1 ea3 ea
tank lid Z407259-1EA 1 ea
Pyrex® rectangular chromatography jar
Corning® 6944
Ground to the close tolerances needed for tight cover fit. Jar edges areground flat within 0.25mm. Covers are not supplied. Do not use heat,pressure or vacuum applications.
Description Cat. No. Qty
L 181 mm × W 238 mm × H324 mm
CLS694411L-1EA 1 ea
L 137 mm × W 162 mm × H267 mm
CLS69444L-6EA 6 ea
TLC & Accessories
TLC AccessoriesTLC Dessicating Cabinets
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9
Aldrich® TLC development chamber
Novel horizontal chambers1 permit the rapid screening of columnchromatography fractions. Designed for economy and use in studentlaboratories. Use with 25 × 70 mm flexible plastic or glass TLC plates thatmay be spotted with up to 10 fractions 7 mm apart along the long edge ofthe plate. Uses only 2 mL solvent with elution complete in about 45 sec(using 20% EtOAc/hexanes as eluant). Greater resolution may be achievedby extending elution time. Borosilicate glass.Performance features:Rapid analysis; highly efficient use of TLC plate; tube wall provides a straightbase for even solvent flow; very little solvent required.
Lit. cited: 1. Levine, S. G., J. Chem. Educ. 73, 77 (1996)
Single slot chamber, L 95.9 mm × O.D. 25.4 mm
Z278629-1EA 1 ea
Z278629-10EA 10 ea
Spectroline UV Viewing Cabinets
Spectroline® CM UV-viewing cabinet
Portable, lightweight aluminum minicabinets with 4 and 6 W removablelamps that may be used for hand-held applications. Lamps provide long-and shortwave irradiation for viewing TLC plates, and for UV fluorescence orabsorption demonstrations. Cabinets have a flexible, contoured viewer forbuilt-in UV-absorbing window, easy-access felt curtains, and removablebottoms for placement over transilluminators for greater intensity andcontrast. CE compliant, UL listed, and meets OSHA requirements.
CM-24: cabinet with removable, combination UV light source (365nm/254nm); 4-watt tubesCM-26: cabinet with removable, combination UV light source (365nm/254nm); 6-watt tubesW × L × H ..................................................................................................................................................... 9 in. × 12 in. × 61=2 in.CSA approved ......................................................................................................................................................................................... Yes
Description Cat. No. Qty
Cabinet CM-24, 115 V, 60 Hz Z169382-1EA 1 ea
Cabinet CM-24, 230 V50 Hz,European 2-pin plug
Z169390-1EA 1 ea
Cabinet CM-26, 115 V, 60 Hz Z169439-1EA 1 ea
Cabinet CM-26, 230 V50 Hz,European 2-pin plug
Z169447-1EA 1 ea
Spectroline® CX™ UV-viewing cabinet
Vinyl-clad aluminum cabinets have separate short- and longwave UV lightsources with specular reflectors for maximum intensity and fluorescentcontrast. Includes three 8 W light tubes; short- and longwave, and whitelight for visible illumination. Cabinets have a flexible, contoured eyepiece forUV-absorbing window, color-coded power buttons, push-buttonwavelength selection, and removable bottom panels for use with trans-illuminators. UL listed and meets OSHA requirements.W × L × H ....................................................................................................................................................... 12 in. × 16 in. × 9 in.
Cabinet CX-20
Description Cat. No. Qty
Cabinet CX-20, 115 V, 60 Hz Z169498-1EA 1 ea
Cabinet CX-20, 230 V50 Hz Z169528-1EA 1 ea
Spectroline® CX™ UV-viewing cabinet replacement parts
See Replacement parts for E-Series lamps for:Longwave UV light tube, Z16,954-4Shortwave UV light tube, Z16,955-2Shortwave Filter assembly, Z16,968-4
Description Cat. No. Qty
8 W, white light Z169560-1EA 1 ea
Longwave Z169579-1EA 1 ea
UV Lamps & Accessories
Spectroline® battery-operated UV lamp
• 4 W longwave bulb• Requires 4 AA batteries (not included)
size ......................................................................................................................................................................... 61=4 in. × 2 in. × 1 in.
Description Cat. No. Qty
Model UV-4B Z284661-1EA 1 ea
Replacement 4 W longwave bulb Z284688-1EA 1 ea
TLC & Accessories
TLC AccessoriesTLC Developing Tanks & Chambers
sigma-aldrich.com/analytical View our current seminar and tradeshow schedule at sigma-aldrich.com/analytical-events
10
Spectroline® E-Series UV lamp
May be used alone (hand-held) or in viewing cabinets. Lamps have onelongwave (365 nm) and one shortwave (254 nm) tube, 230 V.
Description Cat. No. Qty
4 W, 115 V Z169595-1EA 1 ea
6 W, 115 V Z169617-1EA 1 ea
8 W, 115 V Z169633-1EA 1 ea
4 W, 230 V Z169609-1EA 1 ea
6 W, 230 V Z169625-1EA 1 ea
8 W, 230 V Z169641-1EA 1 ea
Replacement Parts for E-Series Lamps
Description Cat. No. Qty
Filter, long/shortwave, 6 W Z169471-1EA 1 ea
Filter, long/shortwave, 8 W Z169684-1EA 1 ea
longwave, 6 W Z169455-1EA 1 ea
longwave, 8 W Z169544-1EA 1 ea
shortwave, 4 W Z169412-1EA 1 ea
shortwave, 6 W Z169463-1EA 1 ea
longwave, 4 W Z169404-1EA 1 ea
shortwave, 8 W Z169552-1EA 1 ea
TLC Plate Racks
Aluminum multi-plate racks
For use with Latch-Lid™ developing chambers.
Description Cat. No. Qty
For use with 10 cm × 10 cm plates Z266043-1EA 1 ea
For use with 20 cm × 20 cm plates Z266035-1EA 1 ea
PTFE multi-plate racks
For use with Latch-Lid™ developing chamber. Can accommodate up to sixTLC plates.
Description Cat. No. Qty
For use with 10 cm × 10 cm plates Z266078-1EA 1 ea
For use with 20 cm × 20 cm plates Z266051-1EA 1 ea
TLC plate rack
Holds two 20 × 20 cm, 10 × 20 cm, or 5 × 20 cm TLC plates in a standardglass developing chamber.
stainless steel rack
Z266027-1EA 1 ea
TLC plate storage racks
Used to carry and store TLC plates without damage to media. Racked platesmay be air or oven dried. Holds up to 10 standard plates in either a verticalor horizontal position. Racks are stainless steel with a carrying handle.stainless steel (20 gauge)
Description Cat. No. Qty
Rack for 10 cm × 10 cm plates Z266108-1EA 1 ea
Rack for 20 cm × 20 cm plates Z266094-1EA 1 ea
TLC plate holder
Horizontal and vertical grooves hold 25 TLC plates of any size in an uprightposition. Lightweight body resistant to usual TLC solvents and chemicals.Great for desiccator storage of plates.
Z265284-1EA 1 ea
TLC & Accessories
TLC AccessoriesUV Lamps & Accessories
For hazardous product information, visit sigma-aldrich.com/safetycenter
11
Adsorbent Scrapers
Adsorbent scrapers
Ideal flat 13 mm steel blade tapered to a sharp edge for rapid removal ofadsorbent from TLC plates. Aluminum handle; replaceable blade.
Z265268-1EA 1 ea
Adsorbent scraper blades
Replacement blades
Z265276-1PAK 5 ea
Cutting Tools
Scoring Tool
for cutting glass plates, tungsten carbide tip
Good for cutting glass plates. Tungsten carbide tip.
23746 1 ea
Diamond glass cutter
nickel plated
Scores/breaks single or double-strength glass. Nickel-plated brass cutterwith wooden handle.
Z169064-1EA 1 ea
Syringes & Pipettes
Hamilton® TLC syringes
PTFE coats the final 3=4 in. of this 2 in. (51 mm) long cemented needle. Thisallows for more reproducible sample spotting on TLC plates.needle L ............................................................................................................................................................................... 51 mm (2 in.)coated tip ............................................................................................................................................................................................................
Description Cat. No. Qty
volume 10 μL, needle size 26s ga,needle type fixed
Z264385-1EA 1 ea
volume 25 μL, needle size 22s ga,needle type fixed
Z264393-1EA 1 ea
volume 50 μL, size, needle size 22ga, needle type fixed
Z264407-1EA 1 ea
volume 100 μL, needle size 22 ga,needle type fixed
Z264415-1EA 1 ea
Kimble® microcapillary pipettes
Disposable pipettes are calibrated —to contain. Pipettes are 5 in. long andmade of soda lime glass. The different sizes are color-coded. Each cylinderpack comes with one pipette device. Capillary action draws the fluid intothe tube. To dispense, simply cover the hole on the rubber bulb andsqueeze.
Description Cat. No. Qty
capacity 5 μL ± 1.0% Z543241-1PAK 250 ea
capacity 10 μL ± 0.5% Z543268-1PAK 250 ea
capacity 20 μL ± 0.5% Z543276-1PAK 250 ea
capacity 25 μL ± 0.5% Z543284-1PAK 250 ea
capacity 50 μL ± 0.5% Z543292-1PAK 250 ea
capacity 100 μL ± 0.5% Z543306-1PAK 250 ea
Other TLC Accessories
TLC saturation pads
Placement of pads with solvent in the development chamber helps toassure rapid and uniform saturation of the atmosphere with solvent vapor.Pads improve reproducibility and reduce "edge effects".
Description Cat. No. Qty
10 cm × 10 cm Z265241-1PAK 100 ea
10 cm × 20 cm Z265233-1PAK 100 ea
20 cm × 20 cm Z265225-1PAK 100 ea
TLC & Accessories
TLC AccessoriesAdsorbent Scrapers
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12
Bulk TLC Adsorbents
Bulk Silica for TLC with Fluorescence
Grade Brand Particle Size Pore Size (Å)Pore Volume (cm3/
g) Surface Area (m2/g) Cat. No. Qty
for thin layerchromatographyhigh-purity grade(Merck Grade 7749)
Merck™ particle size 5 - 40μm
60 - - 346446-1KG 1 kg
for thin layerchromatographyhigh-purity grade
Sigma-Aldrich particle size 2 - 25μm
60 0.75 ~500 288586-1KG 1 kg
for thin layerchromatographyhigh-purity grade
Sigma-Aldrich particle size <20 μmparticle size >632
mesh
60 0.8 500 60765-250G60765-1KG
250 g1 kg
Bulk Silica for TLC without Flurorescence
Grade Brand Particle Size Pore Size (Å)Pore Volume (cm3/
g) Surface Area (m2/g) Cat. No. Qty
for thin layerchromatographyhigh-purity grade
Sigma-Aldrich particle size 5 - 25μm
60 0.75 ~500 288519-250G 250 g
for thin layerchromatographyhigh-purity grade
Sigma-Aldrich particle size 2 - 25μm
60 0.75 ~500 288500-1KG 1 kg
for thin layerchromatographyhigh-purity grade
Sigma-Aldrich particle size 40 μmparticle size >400
mesh
60 0.7-0.85 470-530 60770-1KG 1 kg
for thin layerchromatographyhigh-purity grade
Sigma-Aldrich particle size <20 μmparticle size >632
mesh
60 0.7-0.85 500 60760-1KG 1 kg
Bulk Cellulose for TLC
OOH
OH
O
HO
HO
OOH
OH
O
HO
H
n
High purity cellulose powders for partition chromatography.LOD ................................................................................................................................................................................................................ ≤5%
DS-0, powder, for thin layer chromatography
microcrystalline cellulose for all types of separation chromatography andthin layer electrophoresisign. residue ........................................................................................................................................................................................ ≤0.1%
22197-250G-F 250 g
DFS-0, microcrystalline, for thin layer chromatography
09906-1KG 1 kg
Bulk Polyamide for TLC
Polyamide with Fluorescence
for thin layer chromatography, 6 DF, with fluorescent indicator 254 nmbulk density ............................................................................................................................................................................... 0.25 g/mL
02593-250G 250 g
Bulk FLORISIL for TLC
Florisil® TLC
Silica without Fluorescence
OSi
O
O
Mg2+
Magnesium silicatefor thin layer chromatography
46387-500G-F 500 g
TLC & Accessories
TLC AccessoriesOther TLC Accessories
View our technical presentations at sigma-aldrich.com/analytical-videos
13
Sequential Spraying Makes Drug andLipid Detection Simpler
Drugs such as amphetamines, phenothiazines, and methaqualone canbe sequentially detected on a single plate. After removing the plate fromthe tank, it is dried (5 min, 110 °C), cooled and sprayed with fluorescamine(Cat. No.34653). After 5-10 minutes, amphetamines will exhibit bright greenfluorescence under long-wave UV. The plate is subsequently warmed to110 °C for 1-2 min causing phenothiazines to develop color and tranquilizersto exhibit fluorescence under long-wave UV. Finally, the plate is sprayedwith neutral iodoplatinate (Cat. No. 34651) allowing the detection ofmethaqualone and hydrolyzed methaqualone.
Amphetamines and alkaloids can likewise be sequentially detected onone plate. After removing the plate from the tank, it is dried (5 min, 110 °C),cooled and examined under long-wave UV for quinine, quinine metabolites,and demerol. The plate is next sprayed with ninhydrin (Cat. No. N0757),heated at 90 °C for 10 min and sprayed heavily with diphenyl-carbazone foran intense response from amphetamines and methamphetamines. Theplate is then sprayed with acidic iodoplatinate (Cat. No. I0256), which causesalkaloids to appear as spots of various colors. (To aid in identifying specificcompounds, certain colors can be changed or intensified by oversprayingthe plate with Dragendorff′s reagent (Cat. No. D7518)). Finally, the plate issprayed with ammoniacal silver nitrate and heated (10 min, 110 °C), to allowfor the detection of morphine.
Ninhydrin and Phospray, can be applied sequentially to visualizeseparated lipids. After the plate has been allowed to dry, it is sprayed withninhydrin and gently warmed. Amino containing compounds (phosphatidylethanolamine, etc.) will appear as pink spots. (These spots can be marked toavoid confusion in the next step.) The plate is next cooled to roomtemperature and sprayed with Phospray to reveal phosphorus-containingcompounds (phosphatidyl choline, phosphatidyl ethanolamine etc.).
Spray Reagents
Description Cat. No. Qty
Phospray 33047-U 200 mL
Fluorescamine 34653 100 mL
Bromothymol Blue 34656 200 mL
Dragendorff reagent, for TLCderivatization
44578-100ML-F 100 mL
Iodoplatinate spray reagent
For use in the detection of alkaloids, amines and organic nitrogencompounds.0.15% potassium chloroplatinate and 3% potassium iodide in dilutehydrochloric acid
I9157-100ML 100 mL
Molybdenum Blue spray reagent
For use in the detection of phospholipids and related compounds.1.3% molydbenum oxide in 4.2 M sulfuric acidMolybdenum Blue reagent
M1942-100ML 100 mL
Ninhydrin Fixer Spray Reagent
Acidified ethanol containing 1% (v/v) saturated cupric nitrate.Fixative for ninhydrin chromatograms.store at: 2-8°C
N1411-100ML 100 mL
Ninhydrin Spray Reagent
Detects amino acids, amines and amino sugars.Contains 0.2% ninhydrin in ethanol.store at: 2-8°C
N1286-100ML 100 mL
Phosphomolybdic acid solution
Molybdophosphoric acid[12026‑57‑2] MDL MFCD00011341 H3[P(Mo3O10)4]H3Mo12O40P FW 1825.25
12MoO3 • H3PO4
spray reagent, 10% in ethanol
10% Phosphomolybdic acid in ethanolFor the detection of lipids, steroids, lactones, keto acids, hydroxy acids,unsaturated fatty acids, and phenolic compounds.store at: 2-8°C
P4869-100ML 100 mL
TLC Sprayers
Aldrich® chromatography sprayer
Provide a fine, uniform spray that is optimized for the development of TLCplates. Also suited for use in electrophoresis.
• Adjustable spray pattern using thumb on vent hole• Greaseless, screw-threaded joint will not seize; a simple turn of thethreaded cap pulls joint apart safely
• Threaded SafetyBarbs® prevent accidental breakage during tubinginstallation or removal
• Uses low-pressure gas or air
See the Threaded Ground Glass Tutorial to see how threaded joints work.
Description Cat. No. Qty
10 mL Z529710-1EA 1 ea
50 mL Z529729-1EA 1 ea
125 mL Z529737-1EA 1 ea
250 mL Z529745-1EA 1 ea
TLC & Accessories
Sequential Spraying Makes Drug and Lipid Detection
sigma-aldrich.com/analytical To order, visit sigma-aldrich.com/order
14
Aldrich® flask-type sprayer
Connect using the barb end to a low-pressure gas or air source of <5 psi. Bypositioning your thumb over the vent hole you can control the spraypattern.
Description Cat. No. Qty
75 mL Z190373-1EA 1 ea
250 mL Z129178-1EA 1 ea
Replacement flask, 75 mL Z190381-1EA 1 ea
Replacement flask, 250 mL Z129186-1EA 1 ea
Replacement Head only Z190403-1EA 1 ea
Bottle-type sprayer
capacity 240 mL
Z126306-1SET 1 ea
Nalgene® aerosol spray bottle
capacity 180 mL
Reusable HDPE bottle with PP cap uses air pressure to propel contents fromcontainer. Bottle is filled and pressurized manually. Just pump the cap tocharge the system. Two nozzles are included with each bottle to adjustspray from a fine mist to a heavy stream. Net fill 100 mL bottle.
Z279250-1PAK 4 ea
Preval spray unit
Compact, well-designed spray canister delivers fine mist with CFC-freepropellant. Permits control of reagent delivery, for even coverage andminimal waste. Gas canister serves as handle; cap fits standard 38-400 threadreagent bottles. Kit includes one propellant canister and one 6-ounce glassbottle.
Z365556-1KT 1 ea
Replacement sprayer head for Bottle and Tube-type sprayers
The cap and O-ring are not supplied with the replacement head.See the Threaded Ground Glass Tutorial to see how threaded joints work.
Z407267-1EA 1 ea
Sigma TLC spray box
Completely disposable.Contains knock out vents for better ventilation. Easy to assemble.Compatible with most Sigma sprays.size .................................................................................................................................................................. ~14 in. × 14 in. × 14 in.
S1509-5EA 5 ea
Tube-type sprayer
capacity 50 mL
Tube has hexfoot stand and sprayer head.
Z126292-1SET 1 ea
TLC & Accessories
TLC Sprayers
For hazardous product information, visit sigma-aldrich.com/safetycenter
15
TLC ReagentsSpray reagents allow visualization of the colorless components on a TLC plate. These reagents are mixtures of chemicals that, when sprayed on the plate andallowed to react, will cause colorless compounds to change to colored compounds for easy detection. A spray (visualization) reagent shows selectivity for aspecific functional group or compound type.
CAS No. Compound Cat. No. Qty
- Aerosol® 22 A9753-100MLA9753-500ML
100 mL500 mL
7371-55-3 Aluminum chloride - ethanol solution, ~11% in ethanol/water, for TLC derivatization 28672-100ML-F 100 mL
83-07-8 4-Aminoantipyrine, for spectrophotometric det. of H2O2 and phenols, ≥98.0% 06800-25G06800-100G06800-500G
25 g100 g500 g
524-95-8 2-Aminoethyl diphenylborinate, for TLC derivatization, ≥97.0% 42810-5G42810-25G
5 g25 g
12135-76-1 Ammonium sulfide solution, purum, ~21% in H2O (RT) 09981-100ML09981-500ML
100 mL500 mL
50930-79-5 Aniline phthalate solution, ~0.1 M in butanol/water, for TLC derivatization 08545-100ML-F 100 mL
- Antimony(III) chloride reagent (free of CHC), for thin layer chromatography, ~30% Sb basis 21623-100ML 100 mL
106-51-4 p-Benzoquinone, for spectrophotometric det. of amines, ≥99.5% (HPLC) 12309-25G12309-100G
25 g100 g
366-18-7 2,2′-Bipyridyl, redox indicator, for spectrophotometric det. of Fe, ≥99.0% 14454-5G14454-25G14454-100G
5 g25 g100 g
366-18-7 2,2′-Bipyridyl, purum, ≥98.0% (NT) 14453-25G14453-100G
25 g100 g
7477-67-0 Bispyrazolone, for TLC derivatization, for the det.of cyanide, ≥98.0% 15156-5G 5 g
76-60-8 Bromocresol Green solution, indicator, ready-to-use 02559-500ML 500 mL
128-08-5 N-Bromosuccinimide, ReagentPlus®, 99% B81255-5GB81255-100GB81255-250GB81255-500GB81255-1KG
5 g100 g250 g500 g1 kg
7080-50-4 Chloramine T trihydrate, purum p.a., for the detection of halogens and bromate, ≥98.0% (RT) 23270-50G23270-250G23270-1KG
50 g250 g1 kg
10199-89-0 4-Chloro-7-nitrobenzofurazan, suitable for fluorescence, BioReagent, ≥97.0% (HPLC) 25455-1G25455-5G25455-25G
1 g5 g25 g
7790-94-5 Chlorosulfonic acid, purum, ≥98.0% (T) 26388-100ML26388-500ML26388-1L
100 mL500 mL
1 L
6046-93-1 Copper(II) acetate monohydrate, puriss. p.a., ≥99.0% (RT) 61148-100G61148-500G
100 g500 g
10031-43-3 Copper(II) nitrate trihydrate, puriss. p.a., 99-104% 61194-100G61194-500G
100 g500 g
7758-99-8 Copper(II) sulfate pentahydrate, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., 99-102% 31293-100G31293-500G31293-1KG31293-6X1KG31293-50KG-H
100 g500 g1 kg
6 × 1 kg50 kg
605-65-2 Dansyl chloride, BioReagent, suitable for fluorescence, ≥99.0% (HPLC) 39220-1G-F39220-5G-F39220-50G-F
1 g5 g50 g
86516-36-1 1-Dansylpiperazine, for HPLC derivatization, ≥99.0% 39367-100MG-F39367-500MG-F
100 mg500 mg
119-90-4 o-Dianisidine, for spectrophotometric det. of Au, NO2-, Ce(IV), for the detection of Au, Co, Cu, SCN-, V, ≥97.0% 33430-10G
33430-50G10 g50 g
76-54-0 2′,7′-Dichlorofluorescein solution, indicator, ready-to-use 02591-500ML 500 mL
101-38-2 2,6-Dichloroquinone-4-chloroimide, for spectrophotometric det. of vitamin B6, ≥99.0% 35620-10G35620-50G
10 g50 g
304671-58-7 2,2-Dihydroxy-5-methoxy-1,3-indandione hydrate 341002-500MG 500 mg
100-10-7 4-(Dimethylamino)benzaldehyde, for the determination of hydroxyproline, ≥99.0% (HPLC) 39070-50G39070-250G
50 g250 g
100-10-7 4-(Dimethylamino)benzaldehyde solution, 10 g/L in isopropanol, for TLC derivatization 02560-500ML 500 mL
536-17-4 5-(4-Dimethylaminobenzylidene)rhodanine, for TLC derivatization, ≥98.0% 39090-10G 10 g
95-45-4 Dimethylglyoxime, puriss. p.a., ACS reagent, for the detection of Ni, ≥99.0% (TLC) 40390-25G40390-100G40390-500G
25 g100 g500 g
95-45-4 Dimethylglyoxime, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99% (gravimetric) 33133-25G33133-100G
25 g100 g
119-26-6 2,4-Dinitrophenylhydrazine hydrochloric acid solution, ~0.005 M in ethanol, for thin layer chromatography 18189-100ML-F 100 mL
TLC & Accessories
TLC Reagents
sigma-aldrich.com/analytical For all of your analytical needs, visit us at sigma-aldrich.com/analytical
16
CAS No. Compound Cat. No. Qty
122-39-4 Diphenylamine, puriss. p.a., redox indicator, ACS reagent, reag. Ph. Eur., ≥98% (GC) 33149-100G-R 100 g
39775-75-2 Dragendorff reagent, for TLC derivatization 44578-100ML-F 100 mL
64071-86-9 Fast Black K salt, Standard Fluka 44760
14263-94-6 Fast Blue B Salt, Dye content: ~95% D9805-10GD9805-100G
10 g100 g
1343-88-0 Florisil® TLC, for thin layer chromatography 35483-1KG 1 kg
- Fluorescamine 34653 100 mL
32382-27-7 Fluorescein mercuric acetate, for the determination of disulfide groups, ~90% (UV) 46980-5G-F 5 g
68611-47-2 Fluorescence indicator green 254 nm, for thin layer chromatography 02554-50G02554-250G
50 g250 g
7553-56-2 Iodine, ACS reagent, ≥99.8%, solid 207772-5G207772-100G207772-500G207772-6X500G207772-1KG207772-2.5KG207772-12KG
5 g100 g500 g
6 × 500 g1 kg
2.5 kg12 kg
7705-08-0 Iron(III) chloride, anhydrous, ≥98% 12321-100G12321-1KG12321-2.5KG
100 g1 kg
2.5 kg
7705-08-0 Iron(III) chloride, reagent grade, 97% 157740-5G157740-100G157740-1KG157740-6X1KG157740-2.5KG157740-20KG
5 g100 g1 kg
6 × 1 kg2.5 kg20 kg
51404-69-4 Lead(II) acetate basic, anhydrous, ACS reagent, for sugar analysis according to Horne, ≥33.0% basic Pb (as PbO)basis, ≥75.0% total Pb (as PbO) basis
32306-1KG32306-6X1KG32306-5KG32306-4X5KG32306-25KG-H
1 kg6 × 1 kg
5 kg4 × 5 kg
25 kg
13446-34-9 Manganese(II) chloride tetrahydrate, 99.99% trace metals basis 203734-5G203734-25G203734-100G
5 g25 g100 g
7487-94-7 Mercury(II) chloride, puriss. p.a., ACS reagent, reag. ISO, ≥99.5% (calc. to the dried substance) 31005-100G31005-500G31005-6X500G
100 g500 g
6 × 500 g
50632-57-0 2-Methoxy-2,4-diphenyl-3(2H)-furanone, suitable for fluorescence, ≥98.0% (HPLC) 64958-25MG64958-100MG
25 mg100 mg
38894-11-0 3-Methyl-2-benzothiazolinone hydrazone hydrochloride monohydrate, ≥99.0% (HPLC) 65875-2.5G65875-10G65875-50G
2.5 g10 g50 g
- Millon’s reagent, purum p.a., for the detection of tyrosine 69820-50ML69820-250ML
50 mL250 mL
- Molybdenum Blue spray reagent M1942-100ML 100 mL
654055-01-3 Morin hydrate, for microscopy (Fl.), for the determination of Al, Be, Zn, Ga, In, Sc, water 1 mol/mol 69870-5G69870-10G69870-50G
5 g10 g50 g
1465-25-4 N-(1-Naphthyl)ethylenediamine dihydrochloride monomethanolate, for spectrophotometric det. of nitrate andnitrite, ≥99.0%
70720-25G70720-100G
25 g100 g
485-47-2 Ninhydrin, ACS reagent 151173-10G151173-25G151173-100G
10 g25 g100 g
- Ninhydrin reagent according to Stahl, for thin layer chromatography 17975-100ML 100 mL
- Ninhydrin Spray Reagent N1286-100ML 100 mL
7697-37-2 Nitric acid, puriss. p.a., ACS reagent, fuming, ≥99.5% 78005-500ML-F 500 mL
1083-48-3 4-(4-Nitrobenzyl)pyridine, for TLC derivatization, for spectrophotometric det. of phosphorus-containingpesticides
73210-5G73210-25G73210-250G
5 g25 g250 g
6153-39-5 Orcinol monohydrate, colorimetric detection reagent O1875-5GO1875-10GO1875-100G
5 g10 g100 g
7647-10-1 Palladium(II) chloride, anhydrous, 60% Pd basis 76050-1G76050-5G76050-25G
1 g5 g25 g
12026-57-2 Phosphomolybdic acid solution, ready-to-use spray and plunge reagent, for chromatography 02553-100ML02553-500ML
100 mL500 mL
12026-57-2 Phosphomolybdic acid solution, spray reagent, 10% in ethanol P4869-100ML 100 mL
12501-23-4 Phosphotungstic acid hydrate, for microscopy 79690-25G79690-100G79690-500G
25 g100 g500 g
- Phospray 33047-U 200 mL
TLC & Accessories
TLC Reagents
For technical assistance, visit sigma-aldrich.com/techinfo
17
CAS No. Compound Cat. No. Qty
643-79-8 Phthaldialdehyde, for fluorescence, ≥99.0% (HPLC) 79760-1G79760-5G79760-6X5G79760-50G
1 g5 g
6 × 5 g50 g
2508-19-2 Picrylsulfonic acid solution, ~1 M in H2O 92823-10ML92823-50ML
10 mL50 mL
25910-85-4 Pinacryptol yellow, for photographic purposes 80540-1G 1 g
7758-02-3 Potassium bromide, puriss. p.a., ACS reagent, ≥99.5% (AT) 60093-250G-F60093-1KG-F
250 g1 kg
7778-50-9 Potassium dichromate, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8% 31255-250G31255-1KG31255-50KG-H
250 g1 kg50 kg
16921-30-5 Potassium hexachloroplatinate(IV), puriss., ~40% Pt basis 60260-5G-F 5 g
16921-30-5 Potassium hexachloroplatinate(IV), ≥99.9% trace metals basis 520861-1G520861-5G520861-25G
1 g5 g25 g
7681-11-0 Potassium iodide, BioUltra, ≥99.5% (AT) 60399-100G-F60399-500G-F
100 g500 g
115-41-3 Pyrocatechol Violet, indicator for metal titration 32672-1G32672-5G
1 g5 g
81-88-9 Rhodamine B, for fluorescence 83689-1G 1 g
81-88-9 Rhodamine B solution, 0.2% in isopropanol, for TLC derivatization 02558-100ML 100 mL
7761-88-8 Silver nitrate, puriss. p.a., ≥99.5% (AT) 85228-50G85228-100G85228-250G
50 g100 g250 g
9005-25-8 Starch, from potato, acc. to Zulkowsky (starch, treated with glycerol at 190°C) 85642-25G85642-100G
25 g100 g
121-57-3 Sulfanilic acid, puriss. p.a., ≥99.0% (T) 86090-5G86090-100G86090-500G
5 g100 g500 g
7664-93-9 Sulfuric acid solution, 49-51%, for HPLC 84733-100ML84733-500ML
100 mL500 mL
670-54-2 Tetracyanoethylene, 98% T8809-5GT8809-25G
5 g25 g
10025-69-1 Tin(II) chloride dihydrate, reagent grade, 98% 208035-100G208035-250G208035-500G208035-2.5KG
100 g250 g500 g2.5 kg
6192-52-5 p-Toluenesulfonic acid monohydrate, ReagentPlus®, 98.5% T35920-5GT35920-100GT35920-500GT35920-3KGT35920-15KG
5 g100 g500 g3 kg15 kg
121-44-8 Triethylamine, ≥99.5% 471283-100ML471283-500ML471283-6X500ML471283-2L471283-4L471283-4X4L471283-18L
100 mL500 mL
6 × 500 mL2 L4 L
4 × 4 L18 L
118-12-7 1,3,3-Trimethyl-2-methyleneindoline, liquid, for paper chromatography, ≥96.0% 92550-100ML 100 mL
298-96-4 2,3,5-Triphenyltetrazolium chloride, ≥99.0% (AT) 93140-10G93140-50G
10 g50 g
6159-44-0 Uranyl acetate dihydrate, puriss. p.a., ACS reagent, ≥98.0% (T) 73943-5G73943-25G
5 g25 g
7646-85-7 Zinc chloride, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥98% 31650-100G31650-250G31650-6X250G31650-1KG31650-6X1KG31650-50KG31650-25KG
100 g250 g
6 × 250 g1 kg
6 × 1 kg50 kg
7646-85-7 Zinc chloride solution, 1.0 M in diethyl ether 276839-100ML276839-800ML
100 mL800 mL
TLC & Accessories
TLC Reagents
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