Leukocyte

Endothelial Cells

Astrocyte

Neuron

Microglia

Pericyte

Blood-Brain Barrier & Immune Cell Transmigration

RnDSy-lu-2945

Stimulation

CXCL8/IL-8CCL2/MCP-1

IL-1 RTNF R

TNF-αIL-1β/IL-1F2

Stimulation

Actin Cytoskeleton

Microglia

Brain Tissue

Astrocyte

IL-1β/IL-1F2TNF-αVEGF

CCL2/MCP-1

Astrocyte

CCL3/MIP-1αCXCL8/IL-8

CCL2/MCP-1

Microglia

MMP-2MMP-9

IL-1β/IL-1F2TNF-α

CCL2/MCP-1

Neuron

Pericyte

Brain ECs

Leukocyte I. RollingCerebral Capillary

II. Activation III. Adhesion

IV. Crawling V. Transmigration

IL-1 R

MMP-9

MMP-9

PericyteMigration

Caspase-3Cleavage

IL-1β/IL-1F2

LRP-1

TNF R

TNF-α

● Loss of Pericytes

HSPG

Chemokine

Chemokine R

VCAM-1/CD106

Integrin α4β1(VLA-4)

Integrin αLβ2(LFA-1)

ICAM-1/CD54

● MMP Expression and TJ Protein Degradation

Actions on BBB

Actions on BBB

Actions on BBBActions on BBB

Actions on BBB

● Redistribution of TJ and AJ Proteins● Stress Fiber Formation

● Redistribution of TJ and AJ Proteins● Stress Fiber Formation

● ECM Protein Degradation

CCR2CCL2/MCP-1

PSGL-1/CD162P-Selectin/CD62P

VEGF R2

VEGF

VCAM-1/CD106ICAM-1/CD54CXCL1CXCL2/MIP-2

MMP-9

ECM ProteinDegradation

ECM ProteinDegradation

LeukocyteChemotaxis

LeukocyteChemotaxis

Immune Cell Transmigration Across the Blood-Brain BarrierThe blood-brain barrier (BBB) is a highly specialized, multi-cellular structure that functions as a selective diffusion barrier between the peripheral circulation and the central nervous system (CNS). It is composed of specialized endothelial cells (ECs) that are linked by complex tight junctions (TJs) and adherens junctions (AJs) and is surrounded by astrocytes and pericytes. Under normal conditions, the specialized structure of the BBB hinders paracellular transport of most hydrophilic compounds across the cerebral endothelium and restricts migration of blood-borne cells into the CNS. As a result, resident immune cells, such as microglia, are the initial responders to pathogens or tissue damage. However, prolonged tissue insult triggers inflammatory conditions that cause the BBB to lose its restrictive features, resulting in the subsequent infiltration of peripheral immune cells.

Reactive microglia, astrocytes, and pericytes, as well as ECs, release numerous molecules that promote invasion of peripheral immune cells into CNS. Secreted inflammatory mediators, including CXCL8, CCL2/MCP-1, TNF-a, IL-1b/IL-1F2, recruit immune cells and stimulate the expression of adhesion molecules on ECs that participate in integrin-mediated leukocyte tethering, rolling, and activation. These molecules also trigger the dynamic reorganization of junctional complexes between ECs and EC retraction, thereby promoting the formation of paracellular gaps. Matrix metalloproteases (MMPs) are also released and degrade proteins present in the extracellular matrix and may contribute to the loss of pericytes.

Bio-Techne offers an extensive collection of R&D Systems® products for researching neuroinflammation including antibodies for the detection of specific cell types and immunoassays for the measurement of cytokine levels.

For research use only. Not for use in diagnostic procedures.

View More Detailed Signaling in Brain ECs | rndsystems.com/BBBTransmigration

View full selection of antibodies | rndsystems.com/Antibodies

GFAP in Rat Astrocytes. Glial Fibrillary Acidic Protein (GFAP) was detected in immersion-fixed rat astrocytes using a Sheep Anti-Human GFAP Antigen Affinity-Purified Polyclonal Antibody (Catalog # AF2594). The cells were stained using the NorthernLights™ (NL) 557-Conjugated Donkey Anti-Sheep IgG Secondary Antibody (Catalog # NL010; red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

Integrin aM/CD11b in Human Brain. Integrin aM/CD11b was detected in immersion-fixed paraffin-embedded tissue sections of human brain (cerebral cortex) using a Mouse Anti-Human/Equine Integrin aM/CD11b Monoclonal Antibody (Clone 238446; Catalog # MAB16991). The tissue was subjected to antigen retrieval using the Antigen Retrieval Reagent-Basic (Catalog # CTS013) and stained using the NL557-Conjugated Donkey Anti-Mouse IgG Secondary Antibody (Catalog # NL007; red). Nuclei were counterstained with DAPI (blue). Specific staining was localized to the cytoplasm of microglia (red color). The tissue was double-stained with a Sheep Anti-Human/Mouse/Rat Neurogranin Antigen Affinity-Purified Polyclonal Antibody (Catalog # AF7947) and an Alexa Fluor® 488-conjugated donkey anti-sheep IgG secondary antibody (green).

Species Key: H Human M Mouse R Rat Ca Canine CR Cotton Rat E Equine F Feline MS Multi-species P Porcine Pr PrimateApplication Key: B/N Blocking/Neutralization CD Cell Depletion ChIP Chromatin Immuno precipitation E ELISA FA Functional Assay FC Flow Cytometry ICC Immunocytochemistry IF Immunofluorescence IHC Immunohistochemistry IP Immunoprecipitation SW Simple Western™ WB Western blot

Detect and Identify CellsA variety of cell types are involved in mediating inflammatory responses in neural tissue. Detection and identification of these different cells is essential when investigating neuroinflammation. Bio-Techne offers a wide range of high-performance R&D Systems® antibodies that can facilitate your research.

High-Performance AntibodiesOur extensive antibody portfolio is comprised of over 13,000 high-performance antibodies. Greater than 95% of these antibodies are developed and validated onsite allowing us to ensure that R&D Systems brand antibodies are of the highest quality.

• Guaranteed Performance Rigorous lot specific QC testing supports all applications listed on our datasheets to ensure superior performance.

• Exclusive Clones Hundreds of world-renowned, unique clones, many of which have been used by HLDA to establish CD nomenclature.

• Complete Transparency Interpretable antibody reactivity starts with fully characterized antigens. Unlike our competitors, we provide detailed antigen descriptions on our datasheets.

Neurons Antibodies (Applications)

b-III Tubulin MS (FC, ICC, SW, WB)

Enolase 2/Neuron-Specific Enolase H (IHC, IP, SW, WB) M (IHC, SW, WB)

Synaptophysin H (ICC, IHC, WB) R (ICC, IHC, WB)

Tyrosine Hydroxylase H (ICC, IHC, WB) M (ICC, IF, IHC, WB) R (ICC, IF, IHC, WB) Pr (IF, IHC, WB)

Astrocytes

GFAP H (ICC, SW, WB) R (ICC, SW, WB)

S100B H (IHC, WB)

Microglia

AIF-1/Iba1 H (IHC)

CD68/SR-D1 H (FC, ICC, WB)

Integrin aM/CD11b H (FC, ICC, IHC, WB) M (CD, FC, ICC, IHC, IP) Ca (FC, WB) E (FC, ICC)

Pericytes

PDGF Rb H (B/N, FC, IHC, IP, SW, WB) M (IHC, WB)

a-Smooth Muscle Actin H (FC, ICC, IHC)

B Cells

CD19 H (FC) R (IHC, WB)

MS4A1/CD20 H (FC)

Dendritic Cells

CD11c H (FC, IP, WB) M (FC, WB)

CD83 H (FC, ICC, WB) M (B/N, FC, ICC, WB)

DC-SIGN/CD209 H (B/N, FC, IHC, ICC, WB) M (FC, SW)

Monocytes/Macrophages

CD14 H (B/N, E, FC, IHC, WB) M (FC, WB) E (FC, WB) P (FC, WB)

CD45 H (FC, ICC) M (FA, FC, ICC, IHC, IP, WB)

F4/80/EMR1 M (FC, ICC)

Integrin aM/CD11b H (FC, ICC, IHC, WB) M (CD, FC, ICC, IHC, IP) Ca (FC, WB) E (FC, ICC)

Natural Killer Cells

B3GAT1 R (ICC)

NCAM-1/CD56 H (E, FC, ICC, IHC, WB) M (FC, WB) R (WB)

NKp46/NCR1 H (FA, FC, ICC, WB) M (FA, FC, WB)

Neutrophils

Gr-1/Ly-6G M (CD, FC, ICC, IHC, IP)

Integrin aM/CD11b H (FC, ICC, IHC, WB) M (CD, FC, ICC, IHC, IP) Ca (FC, WB) E (FC, ICC)

Myeloperoxidase H (ICC, IHC, SW, WB) M (ICC, IHC, SW, WB)

T Cells

CD3 (pan T cell marker) H (FA, FC, ICC, IP) M (CD, FA, FC, ICC, IHC, IP)

CD4 (helper T cell marker) H (FC, ICC, IHC, WB) M (CD, FA, FC, ICC, IHC, IP, WB) R (FC, WB) Ca (FC, ICC, WB) CR (FC) F (FC, ICC, WB)

CD8a (cytotoxic T cell marker) H (FC, ICC) M (CD, FA, FC, ICC, IP) Ca (ICC) CR (FC) F (ICC, WB)

CD25/IL-2 Ra (regulatory T cell marker)

H (B/N, E, FC, ICC, IHC, WB) M (B/N, E, FC, IHC, WB) R (FC, ICC, WB) Ca (FC)

FoxP3 (regulatory T cell marker) H (ChIP, FC, ICC, IHC, WB) M (FC, ICC, IHC) R (FC)

View full selection of antibody arrays | rndsystems.com/ProteomeProfiler

Proteome Profiler™ Antibody Arrays Analyze the expression levels of up to 102 cytokines and growth factors in a single sample with R&D Systems® Proteome Profiler™ membrane-based antibody arrays. Broad in scope, the data generated from the arrays can uncover unexpected cellular responses. These assays are ideal for surveying which cytokines and growth factors are present in cell culture supernatants and tissue homogenates.

Survey Cytokine SecretionUnexpected results can be missed when only a subset of proteins is analyzed. Uncover a more complete view of a process by investigating the effects of your experimental conditions on the behavior of multiple cytokines at once. Bio-Techne offers several highly efficient, qualitative tools for simultaneously measuring the levels of multiple proteins in a single sample.

• Cost-Effective Screening Method Each array offers a quick and inexpensive analysis of many analytes simultaneously, in less time than it takes to perform a Western blot.

• No Specialized Equipment Needed They are designed to utilize the same data collection equipment used for Western blots.

• Wide Selection Choose from over 25 arrays for the detection of both intracellular and extracellular factors from a wide variety of sample types.

• User-Friendly Design Each array is stamped with an identification number for easy record keeping and contains reference spots in three corners for orientation purposes.

• Multiple Detection Methods Available We offer arrays that can utilize either chemiluminescence or LI-COR® infrared fluorescence detection*.

Cytokine Expression Induced by IL-1b/IL-1F2 in Mouse Brain. C57BL/6 mice received a bilateral intrahippocampal injection of adeno-associated virus (AAV2) vector expressing either a single chain antibody to phenobarbital (for a control) or IL-1b/IL-1F2. After 4 weeks, brains of the mice were collected, and cytokine expression in brain homogenates was analyzed using the Proteome Profiler™ Mouse Cytokine Antibody Array (Catalog # ARY006). Representative arrays (A) and histogram profiles (B) for select analytes from control (gray bars) and IL-1b/IL-1F2 treated (orange bars) mice. Data were generated by analysis of the mean pixel density of individual antibody spots using image software analysis. Data courtesy of Dr. Jonathan Cherry, University of Rochester Medical Center, Rochester, NY.

Cytokine Profile of Cultured Human Astrocytes. Human primary astrocyte cell cultures were treated with recombinant human IL-1b/IL-1F2 and TNF-a for 24 hours or remained untreated. Conditioned media from the cultures were harvested and assayed for the presence of 36 cytokines using the Proteome Profiler™ Human Cytokine Antibody Array (Catalog # ARY005B). Cytokines that were newly expressed (red boxes) or whose expression changed (blue boxes) following treatment are highlighted. Image from Choi, S.S. et al. (2014) PLoS One 9:e92325.

1. C5/C5a (Complement Component 5/5a)2. slCAM-1 (CD54)3. IL-1β (IL-1F2)4. IP-10 (CXCL10)

5. MIP-1α (CCL3)6. RANTES (CCL5)7. TNF-α (TNFSF1A)8. IL-1ra (IL-1F3)

Cytokines that were Newly Expressed

Cytokines with Changes in Expression Levels

Untreated

IL-1β/IL-1F2 + TNF-α

1. G-CSF (CSFβ, CSF-3)2. GM-CSF (CSFα, CSF-2)3. GROα (CXCL1)4. IL-6

5. IL-8 (CXCL8)6. MCP-1 (CCL2)7. MIF (GIF, DER6)8. Serpin E1 (PAI-1)

Mea

n Pi

xel D

ensi

ty

0

10,000

5000

20,000

15,000

25,000 AAV2-PhenobarbitolAAV2-IL-1β/IL-1F2

GM-CSFCXCL1/KC

CXCL12/SDF-1

sICAM-1/CD54

CCL2/JE/MCP-1

1. Reference Spot2. Reference Spot3. CXCL1/KC4. GM-CSF

5. CCL2/JE/MCP-16. sICAM/CD547. Reference Spot8. CXCL12/SDF-1

1

2

AAV2-Phenobarbitol

7

8

3

4

5

6

AAV2-IL-1β/IL-1F21

8

2

1

2

3

4

5

6

7

Selected Kits/Description

Human Cytokine Antibody Array (Catalog # ARY005B)Detects 36 different cytokines, chemokines, and acute phase proteins.

Human XL Cytokine Antibody Array (Catalog # ARY022)Detects 102 different cytokines, chemokines, and acute phase proteins.

Human Chemokine Antibody Array (Catalog # ARY017)Detects 31 different chemokines.

Mouse Cytokine Antibody Array (Catalog # ARY006)Detects 40 different cytokines, chemokines, and acute phase proteins.

Mouse XL Cytokine Antibody Array (Catalog # ARY028)Detects 111 different cytokines, chemokines, and acute phase proteins.

Mouse Chemokine Antibody Array (Catalog # ARY020)Detects 25 different chemokines.

Rat Cytokine Antibody Array (Catalog # ARY008)Detects 29 different cytokines and chemokines.

B.A.

* Select arrays only.

View full selection of Luminex® assays | rndsystems.com/LuminexAssay

Luminex® Bead-Based Assays We offer two bead-based multiplex immunoassay formats for detecting protein analytes in biological fluids. These R&D Systems® assays utilize Luminex® xMAP® microparticle technology allowing users to better tailor assay selection to their individual research needs. Additionally, our Luminex® assays are specifically designed to optimize the benefits and overcome the challenges of multiplexing.

Detection of Neuroinflammation and Alzheimer’s Disease Biomarkers in Human Serum. The Human Luminex® Screening Assay (Catalog # LXSAH) was used to measure 32 markers of neuroinflammation and Alzheimer’s disease (AD) pathology in human serum samples. Samples were collected from individuals with AD (gray bars; N=20) and from apparently healthy individuals (orange bars; N=20); no medical histories were available. Histogram profiles for select analytes measured at high (A), moderate (B), and low (C) expression levels.

CCL5/RANTES

CHI3L1/YKL-40

ICAM-1/CD54

Seru

m C

once

ntra

tion

(pg/

mL)

0

100,000

200,000

300,000

400,000

CXCL2/GROβCXCL10/IP-10 TNF RII/

TNFRSF1B

IL-23

Seru

m C

once

ntra

tion

(pg/

mL)

0

2000

4000

6000

8000

10,000

CCL3/MIP-1α

CCL4/MIP-1β

IL-1α/IL-1F1

Seru

m C

once

ntra

tion

(pg/

mL)

0

20

60

100

140

120

80

40

AD AverageControl Average

R&D Systems® Luminex® Screening AssaysThese assays are designed to maximize multiplexing capacity and flexibility while maintaining assay specificity.

• Most Flexible Choose from over 214 analytes. All analytes are available in either the polystyrene or magnetic microparticle format.

• Largest Luminex® Multiplex Selection Available These screening assays allow you to maximize the use of your samples by simultaneously analyzing up to 100 analytes.

• Unique Analytes Offered Nearly 30% of our analytes are unique to us.

R&D Systems® Luminex® Performance AssaysThese assays are designed to maximize assay accuracy and precision while preserving the benefits of multiplexing.

• Most Accurate Panel development and validation testing for these assays are similar to our gold-standard Quantikine® ELISA Assays.

• Flexible Select panels are available in either the polystyrene or magnetic microparticle format.

• Customizable to Fit Your Needs Users can choose their analytes of interest from established panels and select “premixed” or “end-user mixed” options.

Luminex® Screening Assays

Human Luminex® Screening AssayChoose from 254 analytes, including 68 different cytokines and chemokines

Mouse Luminex® Screening Assay Choose from 78 analytes, including 40 different cytokines and chemokines

Rat Luminex® Screening Assay Choose from 17 analytes, including 14 different cytokines and chemokines

Selected Luminex® Performance Assays

Human Adhesion Molecule 4-Plex*, ***Detects ICAM-1/CD54, E-Selectin/CD62E, P-Selectin/CD62P, VCAM-1/CD106

Human Cytokine Panel ADetects 22 different cytokines and chemokines

Human Cytokine Panel B*Detects 9 different cytokines and chemokines

Human High Sensitivity Cytokine Panel ADetects 17 different cytokines and chemokines

Human High Sensitivity Cytokine Panel B**Detects 18 different cytokines

Human MMP PanelDetects EMMPRIN/CD147 and 9 different MMP proteins

Multi-species TGF-b PanelDetects TGF-b1, TGF-b2, TGF-b3

Human TIMP 4-plex***Detects TIMP-1, TIMP-2, TIMP-3, TIMP-4

*Analytes in this panel are only available in the polystyrene bead format.

**Analytes in this panel are only available in the magnetic bead format.

***Kit is only available in the premixed format.

A.

B.

C.

View full selection of ELISAs | rndsystems.com/ELISA

Selected ELISA Kits

Analyte Species Catalog #

CCL2/MCP-1

Human DCP00

Mouse, Rat MJE00

Canine CACP00

CCL3/MIP-1aHuman DMA00

Mouse MMA00

CCL20/MIP-3aHuman DM3A00

Mouse MCC200

CXCL1

Human DGR00

Mouse MKC00B

Rat RCN100

CXCL8/IL-8

Human* D8000C

Canine CA8000

Porcine P8000

IFN-g

Human DIF50

Mouse MIF00

Rat RIF00

Canine CAIF00

Porcine PIF00

IL-1b/IL-1F2

Human* DLB50

Mouse MLB00C

Rat RLB00

Porcine PLB00B

IL-6

Human* D6050

Mouse M6000B

Rat R6000B

Canine CA6000

Porcine P6000B

TGF-b1Human DB100B

Mouse, Rat, Canine, Porcine

MB100B

TNF-a

Human* DTA00C

Mouse MTA00B

Rat RTA00

Canine CATA00

Porcine PTA00

Rhesus Macaque RHMTA0

VEGF

Human DVE00

Mouse MMV00

Rat RRV00

Canine CAVE00

Quantikine® ELISA Kits Measure changes in cytokine concentrations using R&D Systems® Quantikine® ELISA Kits. These kits are complete, fully validated, ready-to-run sandwich ELISAs that are designed to measure the concentrations of natural or recombinant analytes. These kits can be used to measure proteins in cerebral spinal fluid, as well as other neural tissue including brain homogenates. In-house manufacturing and extensive validation testing ensure these kits provide the highest levels of specificity, accuracy, precision, and sensitivity, making them the industry gold standard.

Measure Cytokine LevelsMeasuring cytokine production during inflammatory responses can be achieved using R&D Systems® Quantikine® ELISA kits, the gold standard for measuring cytokine concentrations, and R&D Systems® ELISpot Kits, which are used to detect a single cytokine secreting cell. Combine both techniques in one experiment to determine the mean production of your cytokine of interest by a single stimulated cell.

• Legendary Reproducibility Our master calibrated assays ensure that our kits generate accurate data consistently over time. Results today can be compared to last month or even last year, and will be comparable to future results generated.

• Confidence with Controls Our assays have controls with assigned ranges which guarantee performance.

• Unrivaled Reputation Our ELISAs are the most referenced.

• High Specificity Every complete ELISA kit is extensively tested against related molecules and common interfering substances to ensure no cross-reactivity or interference.

• Accurate Detection of Natural Proteins Our antibody pairs recognize the supplied recombinant standard and natural proteins in biological samples in a parallel manner.

*Indicates a Quantikine® High Sensitivity kit is available for this analyte and species.

CCL20/MIP-3a Expression during Acute Pneumococcal Meningitis. CCL20/MIP-3a levels were measured in brain homogenates of C57BL6 mice infected with Streptococcus pneumonia type D39 at various time points after infection using the Mouse CCL20/MIP-3a Quantikine® ELISA Kit (Catalog # MCC200). Brain CCL20/MIP-3a levels were also analyzed in uninfected controls. *P<0.01 compared with uninfected controls. Graph from Klein, M. et al. (2014) PLoS One 9:e93057.

IL-1b/IL-1F2-Induced CXCL8/IL-8 Expression in Astrocytes is Mediated by MAP Kinases. Human astrocyte cell cultures were treated with either an inhibitor specific for p38 (SB202190) or ERK (U0126), or DMSO (control) for 2 hours, followed by treatment with Recombinant Human IL-1b/IL-1F2 (Catalog # 201-LB). The levels of CXCL8/IL-8 in cell culture supernatants were measured using the Human CXCL8/IL-8 Quantikine® ELISA Kit (Catalog # D8000C). CXCL8/IL-8 protein levels were normalized to the number of viable cells as measured using a MTT assay. ***P<0.001. Graph from Mamik, M.K. and A. Ghorpade (2012) PLoS One. 7:e45596.

Unin

fect

edco

ntro

ls

CCL2

0/M

IP-3α

(pg/

mg

Brai

n Pr

otei

n)

0

40

20

6h 24h 36hTime after infection

48h 72h 120h

60

100

80

120*

CXCL

8/M

TT (n

g/m

L)

0IL-1β/IL-1F2 –

–––

–––

––

––

–+

******

******

+++

+

–– –

–

+

–+

+

–––

+

––

+SB202190

U0126DMSO

50

100

150

View full selection of ELISpot kits | rndsystems.com/ELISpot

Cytokine Secretion by Microglia. Microglia were cultured from D1 Sprague Dawley rat pups. After 2 weeks in culture, the primary microglia cell cultures were incubated with Retinoic Acid for 17 hours (orange bars) or remained untreated (gray bars). A. The number of cells secreting TNF-a, IL-1b/IL-1F2, or IL-6 were analyzed using the Rat TNF-a (Catalog # SEL510), Rat IL-1b/IL-1F2 (Catalog # SEL501), or Mouse/Rat IL-6 (Catalog # SEL406) ELISpot Development Modules. B. Histogram profiles of the frequency (expressed as percent) of cytokine secreting cells, which was determined as the number of spots divided by the number of plated cells.

T Cells from Mice Bearing Intracranial Neoplasms Secrete IFN-g. T lymphocytes were isolated from C57BL/6 mice bearing an intracranial neoplasm, which was generated by implanting GL26 mouse glioblastoma cells unilaterally into the right striatum. These intracranial brain tumors were treated with either an intratumoral injection of adenoviral vectors expressing Fms-like Tyrosine Kinase 3 Ligand and Thymidine Kinase (TF; closed symbols) or saline (S; open symbols) 17 days after tumor implantation. The isolated T lymphocytes were incubated with myeloid dendritic cells loaded with either the GL26 (squares) or LLc1 (triangles) tumor antigen, and the number of T lymphocytes secreting IFN-g in response to the tumor antigens was analyzed using the Mouse IFN-g ELISpot Development Module (Catalog # SEL485). *P<0.05 TF versus S. Graph from Curtin, J.F. et al. (2009) PLoS Medicine 6:e1000010.

No Treatment

TNF-α IL-1β/IL-1F2 IL-6

Retinoic Acid

# Cy

toki

ne S

ecre

ting

Cells

(mea

n %

)

0

0.2

0.1

0.3

0.5

0.4

0.6No Treatment

TNF-α IL-1β/IL-1F2 IL-6

Retinoic Acid

IFN

-γ S

pots

S TF

*

GL26

0

50

100

200

150

S TFLLc1

ELISpot Kits Determine the number of cells that secrete your cytokine of interest with R&D Systems® ELISpot Kits. These microplate-based immunoassays are highly sensitive, allowing for the detection of a single cytokine secreting cell among 100,000 cells. Furthermore, use our dual-color ELISpot kits to detect parallel secretion of two cytokines by the same cell. These kits do not require any additional cell expansion or assay refinement.

• Be Confident in the Data Small, crisp spots allow for easier, more accurate quantitation and the largest dynamic range (number of detectable spots/well) on the market.

• Detect Low Frequency Responses Our kits can measure responses with frequencies well below 1 in 100,000 cells – up to 20% more sensitive than the competition.

• Directly Compare Results – Even Years Later We ensure lot-to-lot consistency by manufacturing multiple equivalent lots.

• Choose from Multiple Formats We offer ready-to-use kits for chromogenic and fluorescent detection formats. Do-it-yourself development modules for single analytes are also available.

Selected ELISpot Kits

Analyte Species

Single-Color ELISpot Kits

Dual-Color ELISpot Kits

ELISpot Development Modules

Catalog #s

CXCL8/IL-8Human SEL208

Canine SEL1608

IFN-g

Human EL285 SEL285

Mouse EL485 SEL485

Rat EL585 SEL585

Canine EL781 SEL781

Feline EL764 SEL764

Primate EL961 SEL961

IFN-g (from CD8a+ cells)

Human EL3094

IFN-g (from CD4+ cells)

Mouse EL2019

IFN-g/ Granzyme B

Human ELD5818

Mouse ELD5819

IFN-g/IL-2

Human ELD4506

Mouse ELD5006

Canine ELD6314

Feline ELD8069

Primate ELD7595

IFN-g/IL-4Human ELD5008

Mouse ELD5217

IFN-g/IL-5Human ELD7327

Mouse ELD7420

IFN-g/IL-10 Human ELD5505

IFN-g/IL-13Human ELD7328

Mouse ELD7424

IFN-g/IL-17

Human ELD5219

Mouse ELD5007

Canine ELD6555

Primate ELD7596

IL-1b/IL-1F2Human SEL201

Rat SEL501

IL-6

Human EL206 SEL206

Mouse, Rat

EL406 SEL406

Canine SEL1609

Feline SEL2305

TGF-b1 (Latent) Human EL246 SEL246

TNF-a

Mouse EL410

Rat SEL510

Canine SEL1507

B.

A.

BR_BBB_6734

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