Biotechnology Course Syllabus - Center for Talented Youth · Biotechnology CTY Course Syllabus Day...

Biotechnology CTY Course Syllabus

Day 1, Monday

Morning

Topic/Objective What time? Method?

Introduction − Expectations/Syllabus/Honor Code/Grading/Final Project (Pay it Forward) (20 minutes)

− Ice Breaker (20 minutes)

Pre-Assessment − Test (30-40 minutes)

Introductory Topics Ω What is Biotechnology? Ω History of

Biotechnology Ω The Scientific Method Ω Prokaryotes vs.

Eukaryotes

− PowerPoint Lecture (40 minutes)

Cell Theory (structure vs. function)

− Exercise: Cell City Activity (30 minutes)

The History of DNA − Exercise: Who did what? (30 minutes)

DNA Principles − Exercise: Building DNA Models (30 minutes)

Review − Key principles of the day (15 minutes)

Afternoon


Laboratory Safety − Safety Video? − Safety Contract

Common Laboratory Equipment

− Tour of the Laboratory, PPE

Basic Metric System − Conversion Exercises (20 minutes)

Laboratory Notebook Procedures

− Standard Guidelines Sheet with Examples

Laboratory Techniques − Lab Exercises #1a: Micropipette Techniques (bb_micropipettetech_09) (30 minutes)

− Lab Exercises #1b: Gel Electrophoresis of Dyes (bb_gelelectrophoresisdyes_09) (45 minutes)

Evening


Micropipetting Exercise and Gel Electrophoresis

− Lab Write up in Notebook (30 minutes)

“Pay it Forward” Project − Decide on research topic

Reading Assignment − Chapter 2 Pgs. TBD

Day 2, Tuesday

Morning


Question/Answer − Students can ask questions on any of the key points covered

Key Points from Day 1 − Quiz #1 (Multiple Choice) (20 minutes)

DNA Replication − PowerPoint lecture (40 minutes)

DNA → RNA → Protein Ω Transcription Ω Translation Ω RNA Processing

− Exercise: Enzyme Roulette? (Matching Game/Team Activity) (40 minutes)

Gene Expression/Regulation Ω Prokaryotes vs.

Eukaryotes

− Exercise: Am I on or off? (Group Activity) (30 minutes)

Review − Key principles of the day (30 minutes)

Afternoon


DNA Extraction from Food − Pre-laboratory Exercises (30 minutes) − Laboratory #2: DNALC Strawberry DNA Extraction

(40 minutes)

Evening


DNA Extraction from Cheek Swabs

− Laboratory #3: Genes in a Tube (1 hour)

Reading Assignment − Background on Mitosis and Meiosis (provided by instructor)


Assignment − Mitosis/Meiosis Question/Answer Worksheet

Day 3, Wednesday

Morning


Q & A session − Students questions about key material

Key Points from Day 2 − Quiz #2 (Multiple Choice) (20 minutes)

“Pay it Forward” Research Project

− Submit topic in writing for approval

Chromosomes − PowerPoint Lecture (40 minutes)

Introduction to Mitosis Cell Cycle (Cancer)

− Exercise: Karyotype Diagram (Group Exercise)

Afternoon


Meiosis − PowerPoint Lecture and Diagrams

Differences between Mitosis and Meiosis

− Review the Mitosis/Meiosis Handout given for Study Hall

Inheritance Traits Ω Gregor Mendel

− Lecture/Diagrams Ω Example Problems Ω Handout Genetics Problem Worksheet

Sickle Cell Laboratory − Pre-Laboratory Exercises

Evening


“Pay it Forward” Research − Computer Lab: Start finding information on topic

Genetics − Complete Genetics Problems Worksheet and submit it to the TA

Reading Assignment − Chapter 3 Pgs. TBD

Day 4, Thursday

Morning


Question/Answer − Student questions about key material

Key Points from Days 1-3 − Test #1: Multiple Choice/Short Answer/Essay (40 minutes)

Restriction Enzymes − PowerPoint/Lecture

Afternoon


How Southern Blots Work? (Review)

− Lecture/PowerPoint

How PCR works? (Review) − Pre-Laboratory Exercises (30 minutes)

PCR Amplification Lab − Laboratory #5: Start PCR Amplification of DNA

Evening


Continue PCR Amplification Lab

− Laboratory #5: Finish the PCR Lab and Complete Write-Up

Reading Assignment − Chapter 4 Pgs. 100-121

Day 5, Friday

Morning


Question/Answer Session − Student questions about key material

Key points from Day 4 − Quiz #3: Multiple Choice (20 minutes)

Introduction to Proteins Ω Protein Structure:

Primary, Secondary, Tertiary, and Quaternary.

Ω Methods of Purification

− PowerPoint Lecture

Review − Key Principles of the Day

Afternoon


Enzyme Review − PowerPoint Lecture

Protein Laboratory − Pre-Laboratory Exercises − Laboratory #6: Carolina Cheese Production

BioKit

Evening


Protein Laboratory − Laboratory #6: Finish the Cheese Lab and Complete Write-Up

Reading Assignment − Chapter 5: Pgs. 122-157

Handout Research Papers about GFP

− Read paper and be prepared to summarize how GFP was used in the different model systems

Day 6, Monday

Morning



Key Points from Day 5 − Quiz #4: Multiple Choice (20 minutes)

Microbial Biotechnology Bacterial Transformation Vaccine Research

− PowerPoint Lecture

The Joy of GFP − Exercise: GFP: the amazing gene

Review − Key Principles of the Day

Afternoon


Transformation Procedures − PowerPoint Lecture

Review how transformation works

− Pre-Laboratory Exercises − Laboratory #7: E. Coli with Blue and Green

Fluorescent Proteins

Evening


“Pay it Forward” Research Project

− Work on project − Directed Reading: Chapter 6, 7 Pgs. TBD

Day 7, Tuesday

Morning


Question/Answer Session − Student Questions about key material

Key Points from Day 4, 5, 6 − Test #2: Multiple Choice/Short Answer/Essay

Afternoon


Transformation Procedures − Laboratory #7: E. coli transformation lab finish experiment and complete write-up

Evening


Free study period − Work on Debate Arguments (Group Activity)

Reading Assignment − Crime to Courtroom Handouts (provided by instructor)

− Chapter 8 pgs. TBD

Day 8, Wednesday

Morning



Crime Scene Investigation Ω Chain of Evidence Ω Collection and Types of

Evidence

− Exercise: Mock Crime Scene Ω Role-play Experience (provided by

instructor)


The Scientific Method and Forensic Science

− PowerPoint Lecture − Exercise: ACE-V Fingerprinting Identification

Method and Questioned Document Analysis

Review

Afternoon


Biotechnology and Forensic Science

Ω STRs Ω DNA Fingerprinting Ω mtDNA Analysis

− PowerPoint Lecture − Exercise: RFLP Simulation Activity

DNA Fingerprinting Laboratory − Pre-Laboratory Exercises − Laboratory #8: DNA Fingerprinting Using

Restriction Enzymes

Evening


Free Study Period − Work on Debate Arguments − ”Pay it Forward” Research Projects

Reading Assignment − Chapter 9, 10 Pgs. TBD

Day 9, Thursday

Morning


Question/Answer Session − Student Questions about Key Material

Key Points from Day 7, 8 − Quiz #5: Multiple Choice (20 minutes)

Bioremediation Ω Chemical

Contamination Ω Turning Waste into

Energy

− Discussion: Environmental Accidents and the Solutions

Case Studies

Review

Afternoon


DNA Fingerprinting Laboratory − Laboratory #8: Complete the DNA Fingerprinting Lab and Complete the write-up

Evening


Free Study Period − Work on Debate Arguments − ”Pay it Forward” Research Projects

Reading Assignment − Directed Reading: Chapter 11, 12 Pgs. TBD

Day 10, Friday

Morning



Key Points from Days 7, 8, 9 − Quiz #6: Multiple Choice/Short Answer

Medical Biotechnology Ω Sickle Cell Ω Cancer Ω Stem Cells

− PowerPoint Lecture − Exercise: Blood Typing

Detection Methods Ω Antibodies Ω ELISA

− Exercise: Pedigree Analysis

Afternoon


The Mechanism Behind Retroviruses

− Lecture

ELISA Review − PowerPoint

ELISA Laboratory − Laboratory #9: AIDS Kit I: Simulation of HIV Detection by ELISA

Evening


Free Study Period − Work on Debate Argument − ”Pay it Forward” Research Projects − Finish any previous Lab write-ups

Reading Assignment − Directed Reading Chapter 13 TBD

Day 11, Monday

Morning



Key Points from Day 10 − Quiz #7: Multiple Choice/Short Answer (20 minutes)

Biotechnology Regulations

Review

GMO Foods − Laboratory #10: Identifying GMO Foods Using PCR

Afternoon


GMO Foods − Laboratory #10: Identifying GMO Foods Using PCR

Evening


Free Study Period − Finish “Pay it Forward” Research Project

Day 12, Tuesday

Morning



“Pay It Forward” Research Paper

− Due at the beginning of class

Ethics and Biotechnology

Review for the Final Test − Handout Review sheets and review all the key information (Group Activity Game)

Afternoon


GMO Foods Lab − Laboratory #10: Complete the GMO Foods Laboratory and Write-Up

Evening


Free Study Period − Finish all lab reports/Review all material

Day 13, Wednesday

Morning



Inclusive − Final Test #3: Multiple Choice/Short Answer/Essay

Laboratory Notebook − Lab Notebooks Due before Afternoon Class

Afternoon


Video − GATTACA

Evening


Free Study Period − Finalize debate arguments (Monsanto)

Day 14, Thursday

Morning



Evening


Posters (“Pay it Forward”) − Complete posters for display

Day 15, Friday

Morning


Poster Presentations − Display posters and answer any questions

Biotechnology Course Syllabus - Center for Talented Youth · Biotechnology CTY Course Syllabus Day...

Documents

Transcript of Biotechnology Course Syllabus - Center for Talented Youth · Biotechnology CTY Course Syllabus Day...