Asia%PacificMarine)Biotechnology)Conference)2017) ) ) !!...
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Asia-‐Pacific Marine Biotechnology Conference 2017
(1) The ethyl acetate fraction from Ecklonia cava expression of chemokine and cytokines via down-‐ regulating NF-‐κB and MAPK pathway in TNF-‐α/IFN-‐γ-‐ stimulated HaCaT human keratinocytes. Park, SY1, Kang, N1, Han, EJ2, Kim, HJ3, Lim, YH3, Jang, JT4, Jee, Y5, Jeon, YJ6 Ahn, G1,2,*. 1Department of Marine Bio-‐Food Sciences, Chonnam National University, 2Department of Food Technology and Nutrition, Chonnam National University, 3Seojin Biotech Co., Ltd, 4Aquagreen technology Co., Ltd., 5Department of Veterinary Medicine, Applied Radiological Science Research Institute, Jeju National University, 6Department of Marine Life Science, Jeju National University. Ecklonia cava, an edible brown seaweed is demonstrated to have various biological activities such as anti-‐ inflammatory, anti-‐oxidant and cytoprotective effects. In this study, we evaluated the anti-‐inflammatory potential and mechanisms of the ethyl acetate fraction from E. cava (ECEF) in the tumor necrosis factor-‐α (TNF-‐α)/interferon-‐γ (IFN-‐γ)-‐stimulated inflammatory response in HaCaT human keratinocytes. To measure the effects of ECEF on chemokine and pro-‐inflammatory cytokine expression in HaCaT cells, we used the following methods: cell viability assay, reverse transcription-‐polymerase chain reaction, and western blotting. The result that ECEF did not show cytotoxicity to HaCaT cells at the tested concentrations. ECEF suppressed TNF-‐α/IFN-‐γ-‐stimulated mRNA expression of chemokines and pro-‐inflammatory cytokines. In addition, ECEF inhibited the activation of NF-‐ κB and extracellular signal-‐related kinases (ERK)/p38 MAPK signaling pathways. ECEF exerts anti-‐inflammatory effects by suppressing the expression of TNF-‐α/IFN-‐γ-‐ stimulated chemokines and pro-‐inflammatory molecules via a blocked NF-‐κB, ERK and p38 activation. Our results suggest that ECEF might be a useful therapeutic candidate for inflammatory skin diseases. (2) Venoms to Drugs. Alewood P*. Institute for Molecular Bioscience, University of Queensland, Brisbane Australia. Many organisms including snakes, spiders, scorpions and cone snails have evolved venom for prey capture or defence1. These venoms typically contain a complex cocktail of bioactive disulfide bond rich polypeptide toxins that target a wide range of receptors including enzymes, ion channels, GPCRs and transporters. Of interest to drug designers is their high potency and selectivity combined with their resistance to many proteases. Of particular interest are venoms from the marine Conidae2,3, with smaller polypeptide chains of 10-‐40 amino acids that are highly constrained by one to five disulfide bridges and are structurally well defined. Their high potency and exquisite selectivity for ion channels and
receptors has led to two drug candidates from our laboratories. In this presentation I will outline our program of discovery, describe the amazing diversity of molecular structures being discovered and the regioselective chemistry developed to facilitate disulfide bond formation. This has led to mimetics that have similar or improved potency to the native molecule plus exceptional stability when exposed to reducing environments and in plasma. Together, these results underpin the development of more stable and potent peptide mimetics suitable for new drug therapies, and highlight the application of this technology more broadly to disulfide bonded peptides and proteins. References: 1. Sébastien Dutertre, Ai-‐Hua Jin, Irina Vetter, Brett Hamilton, Kartik Sunagar, Vincent Lavergne, Valentin Dutertre, Bryan Fry, Agostinho Antunes, Paul F. Alewood and Richard J. Lewis. Nature Communications 5:3521, 2014. 2. Akondi KB, Muttenthaler M, Dutertre S, Kaas Q, Craik DJ, Lewis RJ, Alewood PF (2014). Chemical Reviews 114 (11) 5815. 3. I Sharpe, J Gehrmann, M Loughnan, L Thomas, D Adams A Atkins, DJ Craik, D Adams PF Alewood and RJ Lewis (2001). Nature Neuroscience, 4(9) 902. (3) Algal-‐biorefinery: challenges and opportunities. Angelidaki, I* and Alvarado-‐Morales, M. Department of Environmental Engineering, Technical University of Denmark. The recent years challenges such as climate change and environmental problems require action and strategic planning in the future to ensure access to food, feed and energy. Therefore, there is a need for alternative sources of biological-‐based products. Algal biomass is a not completely explored "treasure" and has been considered ad possibility to address these future challenges. The technological potential of the combined use of micro-‐and macro-‐algae for the production of various biological products is great. Macroalgae can be used directly as food, snack, as sprinkle in salads and as additive for flavor enhancing. Microalgae such as Spirulina and Chlorella are already used with great success as nutritional supplements. Food additives (such as alginate) may be extracted from macroalgae and the remainder of the biomass can then be used for bio-‐and fertilizer without any waste product. Micro-‐and macro-‐algae have a high content of biochemicals such as vitamins and antioxidants, which can be used to fortify foods and protect the more unsaturated fatty acid (omega-‐3) to be converted into saturated fatty acids or rancid.
Asia-‐Pacific Marine Biotechnology Conference 2017
In a biorefinery concept algae can be used for the production of biomass, functional food, feed, biochemicals and bioenergy. In addition, algae can with advantage be used as fertilizer for crops. This applies both to the use of algal waste products from biofuel production, but also from blue-‐green algae that can grow in the wet rice fields and fix nitrogen from the air and thus provide plants with nitrogen, without the need for application of fertilize