Arabidopsis
10
Arabidopsis Oryza sativa Zea mays PR5 kinase PR5 inositol monophosphatase PR5 lecithin acetyltransferase PR5 HGTP anticodon Vitis vinifera PR5 cytochrome P450 PR5 ELO PR-5 Fusion Proteins Of Plants Adv Bot Res (2009) 17: 439-489.
description
PR-5 Fusion Proteins Of Plants. Arabidopsis. Oryza sativa. Zea mays. PR5 kinase. PR5 inositol monophosphatase. PR5 lecithin acetyltransferase. PR5 HGTP anticodon. Vitis vinifera. PR5 cytochrome P450. PR5 ELO. Adv Bot Res (2009) 17: 439-489. - PowerPoint PPT Presentation
Transcript of Arabidopsis
Arabidopsis Oryza sativa Zea mays
PR5 kinase
PR5 inositol monophosphatasePR5 lecithin acetyltransferase
PR5 HGTP anticodon
Vitis vinifera
PR5 cytochrome P450
PR5 ELO
PR-5 Fusion Proteins Of Plants
Adv Bot Res (2009) 17: 439-489.
Known Functions Of PR-5/Thaumatin Like Proteins
PR5L/TLP
(PR-5/thaumatin Like)
Antifungal
Beta-glucanase
αAI/Trypsin inhibitor
Antifreeze
Cytokinin-binding
Xylanase inhibitor
Actin binding
Protein-Protein or/and Carbohydrate-Protein Interactions Leading To………?
Osmotin Is An Antifungal Tobacco PR-5 Protein
•Induced by pathogen attack
•Antifungal in planta
•Specific broad-spectrum antifungal activity
C. albicans S. cerevisiae
F. oxysporum P. infestans
S. cerevisiae IZH2 Encodes A GPCR-Like Protein That Increases Osmotin
Sensitivity
• Plasma membrane has apoptosis signaling ‘receptor’
• Apoptosis is the major mechanism of toxicityMolecular Cell (2001) 8: 921-930.
stress responses
Osmotin
CW
PM
Osmotin
RAS2/cAMP
Apoptosis
┴
ROS↑Molecular Cell (2005) 17: 171-180.
IZH2/PHO36
Alignment: Untitled1
....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| 10 20 30 40 50 60 70 0000000000 ---------- --MSSHKGSV VAQGNGAPAS NREADTVELA ELGPLLEEKG KRVIANPPKA EEEQTCPVPQ 0000000001 MNEPTENRLG CSRTPEPDIR LRKGHQLDGT RRGDNDSHQG DLEPILEASV LSSHHKKSSE EHEYSDEAPQ 0000000002 ---------- ---------- ---------- ---------- -MSTLLERTK SVQELKKRAA GKTSANPAE- Consensus LE
....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| 80 90 100 110 120 130 140 0000000000 EEEEEVRVLT LPLQAHHAME KMEEFVYKVW EGRWRVIPYD VLPDWLKDND YLLHGHRPPM PSFRACFKSI 0000000001 EDEG-FMGMS PLLQAHHAME KMEEFVCKVW EGRWRVIPHD VLPDWLKDND FLLHGHRPPM PSFRACFKSI 0000000002 ---------- ---------- -----VAKAK KVLRRLYSWD EIPEWQRDND FILHGYVKET SSFIETFKSL Consensus V K R D P W DND LHG SF FKS
....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| 150 160 170 180 190 200 210 0000000000 FRIHTETGNI WTHLLGFVLF LFLGILTMLR PNMYFMAPLQ EKVVFGMFFL GAVLCLSFSW LFHTVYCHSE 0000000001 FRIHTETGNI WTHLLGCVFF LCLGIFYMFR PNISFVAPLQ EKVVFGLFFL GAILCLSFSW LFHTVYCHSE 0000000002 FYLHNESVNI YSHLIPALGF FTVLLLDKST IKVFATTTWL DHMVIDLFYS GAFACLILSS SFHCLKSHSL Consensus F H E NI HL F V F GA CL S FH HS
....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| 220 230 240 250 260 270 280 0000000000 KVSRTFSKLD YSGIALLIMG SFVPWLYYSF YCSPQPRLIY LSIVCVLGIS AIIVAQWDRF ATPKHRQTRA 0000000001 GVSRLFSKLD YSGIALLIMG SFVPWLYYSF YCNPQPCFIY LIVICVLGIA AIIVSQWDMF ATPQYRGVRA 0000000002 RIATLGNKLD YLGICILIVT SMVSILYYGY FEKFSLFCLF ALITVSFGIA CSIVSLKDKF RKREWRPYRA Consensus KLD Y GI LI S V LYY GI IV D F R RA
....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| 290 300 310 320 330 340 350 0000000000 GVFLGLGLSG VVPTMHFTIA EGFVKATTVG QMGWFFLMAV MYITGAGLYA ARIPERFFPG KFDIWFQSHQ 0000000001 GVFLGLGLSG IIPTLHYVIS EGFLKAATIG QIGWLMLMAS LYITGAALYA ARIPERFFPG KCDIWFHSHQ 0000000002 GLFVCFGLSS IIPIFSGLYC YSFSEIWTQI QLFWVLLGGV LYIIGAVLYG MRFPEKICPG KFDIWGHSHQ Consensus G F GLS P F T Q W L YI GA LY R PE PG K DIW SHQ
....|....| ....|....| ....|....| ....|.. 360 370 380 0000000000 IFHVLVVAAA FVHFYGVSNL QEFRYGLEGG CTDDTLL0000000001 LFHIFVVAGA FVHFHGVSNL QEFRFMIGGG CSEEDAL0000000002 LFHFLVVIAA LCHLRGLLNS YELVHIKMEN GIVS---Consensus FH VV A H G N E
Human AdipoR1Human AdipoR2Yeast PHO36Consensus
Human AdipoR1Human AdipoR2Yeast PHO36Consensus
Human AdipoR1Human AdipoR2Yeast PHO36Consensus
Human AdipoR1Human AdipoR2Yeast PHO36Consensus
Human AdipoR1Human AdipoR2Yeast PHO36Consensus
Human AdipoR1Human AdipoR2Yeast PHO36Consensus
IZH2 Is Homologous To Mammalian Adiponectin Receptors, AdipoR1 and AdipoR2
Adiponectin gC1q Domain (Green) And Osmotin (Purple) Structures Can Be
Overlapped
Molecular Cell (2005) 17: 171-180
gC1qcollagen
Adiponetin
Occurence of PR-5/Thaumatin-like Proteins
•Sequence and structural similarity with plant PR-5 proteins•As families
Yes No
Flowering plants (All)Mosses (All)Algae (All)Amoeba (Some)Fungi (Most)Insects (Some)Bacteria (Some)Cyanobacteria (Some)Viruses (Some)
Mammals (All)Bony fishes (All)Birds (All)
Sp
ecie
s
Adv Bot Res (2009) 17: 439-489.
•As families
Sp
ecie
s
Adv Bot Res (2009) 17: 439-489.
Occurence of C1q Proteins
No Yes
Flowering plants (All)Mosses (All)Algae (All)
Mammals (All)Bony fishes (All)Birds (All)Amoeba (Some)Fungi (Most)Insects (Some)Bacteria (Some)Cyanobacteria (Some)Viruses (Some)
Do C1q Domain Proteins and Thaumatin Domain Proteins Have Similar Functions?
•Most organisms have either C1q domain proteins or thaumatin domain proteins, not both•Osmotin signals in yeast through the homolog of the receptor of a mammalian C1q domain protein called adiponectin
ANTI-Diabetic ANTI-Atherosclerotic
Adiponectin Is The Ligand Of AdipoR1/R2 And It Has Beneficial Effects On Human Health
ANTI-Inflammatory Bowel Disease/Colitis
ANTI-Cancer
AMPK-P
PM
gC1q
AdipoR1/AdipoR2 gC
1q
collag
en
Ad
ipo
ne
tin
collagen
