Aβ42 O (1µM) Surface  1 Total  1 A B ** 25 A β42 M FITC- A β42 M A β42 O FITC- A β42 O...

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Transcript of Aβ42 O (1µM) Surface  1 Total  1 A B ** 25 A β42 M FITC- A β42 M A β42 O FITC- A β42 O...

  • A42O (1M)Surface b1Total b1AB**25

    A42MFITC-A42M

    A42OFITC-A42O1510A42MA42DiA42TriA42Tet - +CSupplemental Fig. S1

  • ###Supplemental Fig. S2

  • VinculinActinVinculinActinA42O (M)B - 0.25 2 - 0.25 22h4hCytoFCC*** TubulinVincTalin TubulinVincTalin - + AFCCytoA42OSupplemental Fig. S3DAPP/PS1 WT Scale bar 30m**E*Focal Vinculin / Cytosolic VinculinFC Vinculin (% Cont)

  • CytoFCA42O - + + - - - + + Cof siRCofilinP-CofVinculinCofilinP-CofVinculinAB**Supplemental Fig. S4FC Vinc (% Cont)

  • Supplemental Fig. S5

    NC (n=4)AD (n=5)Gender2M & 2F2M & 3FAge Range82-9582-96Average Age (+SD)88.75 + 6.7088.4 + 5.94PMI Range (h)3.87-8.63.0-7.8Average PMI (+SD)5.63 + 2.154.46 + 1.93

  • Vehicle A42O (1uM)Nox inhibitorHUTS-4MAB1965VehicleH2DCF Supplemental Fig. S6A

  • A42O +b1 IgGA42O

    Control

    Annexin VDAPI PI / DAPI

    BC**HControlAb42OAb42O + MAB1965PIDSupplemental Fig. S6 Continued

  • P-CofActinA42O - + + + VehVehMAB1965TroloxIP: 14-3-3SSH1BLysateCofSSH1SSH1Beads14-3-3Supplemental Fig. S7AA42OCbeads - - +CofSSH1SSH1IP:SSH1CofLysateIPD**Beads14-3-3IP:14-3-3

  • Supplemental Fig. S8WT GFAPDAPIPSD95DAPISynapsin I

    Cofilin+/-A DGCA1CA3 Scale bar 300mCA3 SLSLB