Aβ42 O (1µM) Surface 1 Total 1 A B ** 25 A β42 M FITC- A β42 M A β42 O FITC- A β42 O 15 10 A...
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0 25 50 75 100 125 C ont A 42 O Surface 1 /Total (% C ont) Aβ42 O (1µM) Surface 1 Total 1 A B ** 25 Aβ42 M FITC- Aβ42 M Aβ42 O FITC- Aβ42 O 15 10 Aβ42 M Aβ42 Di Aβ42 Tr i Aβ42 Te t - + C Supplemental Fig. S
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Transcript of Aβ42 O (1µM) Surface 1 Total 1 A B ** 25 A β42 M FITC- A β42 M A β42 O FITC- A β42 O 15 10 A...
0
25
50
75
100
125
Cont
A42O
Su
rfac
e
1 /
To
tal
(% C
on
t)
Aβ42O (1µM)
Surface 1
Total 1
A B
**
25
Aβ42M
FITC-Aβ42MAβ42O
FITC-Aβ42O
1510
Aβ42M
Aβ42Di
Aβ42Tri
Aβ42Tet
- +
C
Supplemental Fig. S1
0 20 40 60 80 1000.0
0.1
0.2
0.3
0.4Biotin-A42M
Biotin-A42O
Biotin-A42F
Biotin-A42 (nM)
Sp
ecifi
c B
ind
ing
(O
D)
#
#
#
Supplemental Fig. S2
Vinculin
Actin
Vinculin
Actin
Aβ42O (µM)
B - 0.25 2 - 0.25 2
2h 4h
Cy
toF
C
C
***
Tubulin
Vinc
Talin
Tubulin
Vinc
Talin
- + A
FC
Cy
to
Aβ42O
Supplemental Fig. S3
0
25
50
75
100
125
Cont
A42O (0.25M)A42O (2M)
FA
Vin
culin
(%
Co
nt)
D
0
25
50
75
100
125
WTAPP/PS1
Fo
cal V
inc
Are
a(%
WT
)
APP/PS1 WT
Scale b
ar 30μm 0
25
50
75
100
125
WTAPP/PS1
Fo
cal V
inc
Siz
e(%
WT
)
**
E
*
Fo
ca
l V
inc
uli
n /
C
yto
so
lic
Vin
cu
lin
FC V
incu
lin (%
Con
t)
0
25
50
75
100
125
Cont
A42O (0.25M)A42O (2M)
FA
Vin
culin
(%
Co
nt)
Cy
toF
C
Aβ42O - + + -
- - + + Cof siR
Cofilin
P-Cof
Vinculin
Cofilin
P-Cof
Vinculin
A
0
25
50
75
100
125
Cont siR (Veh)
Cont siR (A42O)Cof siR (Veh)
Cof siR (A42O)
FA
Vin
c (%
Co
nt)
B
**
Supplemental Fig. S4
FC V
inc
(% C
ont)
0
25
50
75
100
125
Cont siR (Veh)
Cont siR (A42O)Cof siR (Veh)
Cof siR (A42O)
FA
Vin
c (%
Co
nt)
NC (n=4) AD (n=5)
Gender 2M & 2F 2M & 3F
Age Range 82-95 82-96
Average Age (+SD) 88.75 + 6.70 88.4 + 5.94
PMI Range (h) 3.87-8.6 3.0-7.8
Average PMI (+SD) 5.63 + 2.15 4.46 + 1.93
Supplemental Fig. S5
Vehicle Aβ42O (1uM)
Nox inhibitor HUTS-4 MAB1965Vehicle
H2D
CF
Supplemental Fig. S6
A
Aβ
42O
+1
Ig
GA
β42
OC
on
tro
l
Annexin VDAPI
PI / DAPI
Cont A
1 Ig
G
+
A
0
20
40
60
Lat
e A
po
pto
tic
(%)
0
5
10
15
20
25
Ear
ly A
po
pto
tic
(%)
B C
**
H
Control A42O A42O + MAB1965
PI
D
Supplemental Fig. S6 Continued
P-Cof
Actin
Aβ42O - + + +
Ve
h
Ve
h
MA
B1
96
5
Tro
lox
IP: 14-3-3SSH1
B
Ly
sa
teCof
SSH1
SSH1
Be
ad
s
14
-3-3
Supplemental Fig. S7
A
Aβ42O
C
be
ad
s
- - +
Cof
SS
H1
SS
H1
IP:
SSH1
Cof Ly
sa
teIP
D
0
100
200
300
400
Cont
A42O
SS
H1-
Co
f C
om
ple
x(%
Co
nt)
**
Be
ad
s
14
-3-3
IP:
14-3-3
Supplemental Fig. S8
Cofilin
Cofilin
Actin
Actin
WT
WT
Cofili
n +/
-Cof
ilin
+/-
HIP
PO
CT
XWT
GF
AP
DA
PI
PS
D9
5D
AP
IS
yn
ap
sin
I
Cofilin+/-
A
DG
CA1
CA3
Scale bar
300μm
CA3
SL
SL
B
