HEL/DC T

h17

HEL/DC T

h17 +

αIL-23R

HEL/DC T

h17 +

αIL-12p40

PbAb Th17

PbAb Th17 +

HEL/ DC

0

2

4

6

8

10

ng/m

l

Supplemental Figure 1. Production of IL-22 by CD4 cultures activated by various stimuli, as indicated. IL-22 levels were determined by ELISA in the culture supernatant, on day 4.

Supplemental Fig.1

HEL/APC T

h17

PbAb Th170

100

200

300

400

Rel

ativ

e Ex

pres

sion

HEL/APC T

h17

PbAb Th170

200

400

600

Rel

ativ

e Ex

pres

sion

HEL/APC T

h17

PbAb Th170

100

200

300

400

500

Rel

ativ

e Ex

pres

sion

Supplemental Fig.2

CD40L ICOS A AHR

HEL/APC T

h17

PbAb TH170

5

10

15

pg/m

l

HEL/APC T

h17

PbAb Th170.0

0.2

0.4

0.6

0.8

1.0

ng/m

l

HEL/APC T

h17

PbAb Th170

10

20

30

40ng

/ml

GM-CSF IL-21 IL-17F B

Supplemental Figure 2. Production of immune-related molecules by HEL/APC and PbAb Th17 cells, after 4 days of incubation in culture. (A) Expression of transcripts of the indicated molecules by cells of the two Th17 sub-populations, determined by qPCR. (B) Levels of the indicated molecules, measured by ELISA, in supernatants of cultures of the two sub-populations.

Supplemental Fig.3

Proliferation Assay

Day 2

Day3

Day4

0

50

100

150

200

250 HEL/APC Th17PbAb Th17

CPM

(x1,

000)

Supplemental Figure 3. Th17 stimulated by HEL/APC or PbAb proliferate with different kinetics. Proliferation was measured by 3H-Thymidine incorporation at different time points, as indicated, using conventional methods. Control CD4 cell cultures, incubated with the polarizing cytokine cocktail, but with no stimulation, incorporated <200 CPM.