SDS –PAGE Loading sample buffer

2x Loading sample buffer (10ml) 1 M Tris-HCl pH 6.8 1 ml 10% SDS 4 ml Glycerol 2 ml β-mercaptoethanol 2.5 ml 1% Bromophonol blue 500 µl dH2O to 10 ml 4x Loading sample buffer (10ml) 1 M Tris-HCl pH 6.8 2 ml SDS 0.8 g Glycerol 4 ml β-mercaptoethanol 4 ml 10% Bromophonol blue 10 µl