ACSF2 Polyclonal antibodyCatalog Number:16140-1-AP

For Research Use Only

www.ptglab.com

Basic Information Catalog Number:16140-1-AP

Size:150UL , Concentration: 300 μg/ml byBradford method using BSA as thestandard;

Source:Rabbit

Isotype:IgG

Immunogen Catalog Number:AG9199

GenBank Accession Number:BC012053

GeneID (NCBI):80221

Full Name:acyl-CoA synthetase family member2

Calculated MW:615 aa, 68 kDa

Observed MW:55-70 kDa

Purification Method:Antigen affinity purification

Recommended Dilutions:WB 1:500-1:1000 IHC 1:20-1:200 IF 1:20-1:200

Applications Tested Applications:IF, IHC, WB,ELISA

Species Specificity:human, mouse, rat

Note-IHC: suggested antigen retrieval withTE buffer pH 9.0; (*) Alternatively, antigenretrieval may be performed with citratebuffer pH 6.0

Positive Controls:

WB : mouse lung tissue,

IHC : human skin tissue,

IF : A549 cells,

Background Information

Storage Storage:Store at -20°C. Stable for one year after shipment.Storage Buffer:PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Aliquoting is unnecessary for -20ºC storage

T: 1 (888) 4PTGLAB (1-888-478-4522) (toll freein USA), or 1(312) 455-8498 (outside USA)

E: proteintech@ptglab.com W: ptglab.com

For technical support and original validation data for this product please contact: This product is exclusively available under ProteintechGroup brand and is not available to purchase from anyother manufacturer.

Selected Validation Data

mouse lung tissue were subjected to SDS PAGEfollowed by western blot with 16140-1-AP (ACSF2Antibody) at dilution of 1:600 incubated at 4 degreecelsius over night.

Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 16140-1-AP (ACSF2 Antibody) at dilution of 1:50 (under 10xlens).

Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 16140-1-AP (ACSF2 Antibody) at dilution of 1:50 (under 40xlens).

Immunofluorescent analysis of (4% PFA) fixedA549 cells using 16140-1-AP (ACSF2 antibody) atdilution of 1:50 and Alexa Fluor 488-conjugatedAffiniPure Goat Anti-Rabbit IgG(H+L).