Post on 23-Jan-2016
Figure S1
LC3
SQSTM1
ACTB
con 0.5 μM 1 μM
HCT116 cells
SAHA
HepG2 cells
con 1 μM 3 μM
SAHA
LC3
SQSTM1
ACTB
con 0.5 μM 1 μM
HCT116 cells
SAHA
FOXO1
CDKN1A
ACTB
HepG2 cells
con 1 μM 3 μM
SAHA
FOXO1
CDKN1A
ACTB
Figure S2
A
HCT116 cells
con 0.125 0.25 μM
TSA
FOXO3
ACTB
FOXO4
HepG2 cells
con 0.25 0.5 μM
TSA
FOXO3
ACTB
FOXO4
B
HCT116 cells HepG2 cells
TSA - 0.125 0.25 μM - 0.125 0.25 μM - 0.25 0.5 μM - 0.25 0.5 μM
FOXO3 FOXO4 FOXO3 FOXO4
Figure S3
con 0.125 0.25 0.5 μM
FOXO1
TP53
CDKN1A
ACTB
TSA
con 0.125 0.25 0.5 μM
TSA
TP53+/+ HCT116 cell TP53-/- HCT116 cell
Figure S4
SQSTM1
BAF - - - - + + + +
HepG2 cells
FOXO1
LC3
ACTB
si-con si-FOXO1 si-con si-FOXO1
TSA - + - + - + - +
Figure S5
FOXO3
LC3
SQSTM1
ACTB
si-con si-FOXO3
TSA - + - +
FOXO4
LC3
SQSTM1
ACTB
si-con si-FOXO4
TSA - + - +
Figure S6
B
A
ATG4B ATG12
PIK3C3 BECN1
Figure S7
FOXO1
p-RPS6
si-con si-FOXO1
HepG2 cells
TSA - + - +
RPS6
p-EIF4EBP1
EIF4EBP1
ACTB
Figure S8
HCT116 cells
SAHA - + - + - + CQ BAF
Figure S9
A
C TSA - + - +
si-con si-ATG7
ATG7
Cleaved CASP3
ACTB
Cleaved PARP1
control TSA
si-con
si-ATG7
200 μm
B
TSA - + - +
Figure S10
B TSA - + - +
si-con si-FOXO1
FOXO1
Cleaved CASP3
ACTB
Cleaved PARP1
si-con si-FOXO1
control TSA control TSA
A
200 μm
Figure S11