Fig. S1. Effect of TG2 expression on LNCaP cell growth (A) and

LNCaP LNCaP E.V.

LNCaP TG2

Fig. S1. Effect of TG2 expression on LNCaP cell growth (A) andKaryotyping of parental, vector-infected and TG2-transfected LNCaP cells (B)

LNCaP LNCaP-EV LNCaP-TG2

Histones

IκBα

EV LNCaP

C N C N C N C N

Fig. S2. Co-localization of TG2 and NF-κB in nuclear fraction of prostate cancer cells (A). Transient down regulation of NF-kB partially restored AR expression in PC3 cells (B). Putative NF-κB binding sites in the promoter of AR gene (C and D).

Exon 1

Cont si

-38 bpGACCCGACTCGCAAACTGTTGCATTTGCTCTCCACCTCCCAGCGCCCCCTCCGAGATCCCGGGGAGCCAGCTTGCTGGGAGAGCGGGACGGTCCGGAGCAAGCCCAGAGGCAGAGGAGGCGACAGAGGGAAAAAGGGCCGAGCTAGCCGCTCCAGTGCTGTACAGGAGCCGAAGGGACGCACCACGCCAGCCCCAGCCCGGCTCCAGCGACAGCCAACGCCTCTTGCAGCGCGGCGGCTTCGA AGCCGCCGCCCGGAGCTGCCCTTTCCTCTTCGGTGAAGTTTTTAAAAGCTGCTAAAGACTCGG +246 bp

-760 bpGGGTGATTTTGCCTTTGAGAGTCTGGATGAGAAATGCATGGTTAAAGGCAATTCCAGACAGGAAGAAAGGCAGAGAAGAGGGTAGAAATGACCTCTGATTCTTGGGGCTGAGGGTTCCTAGAGCAAATGGCACAATGCCACGAGGCCCGATCTATCCCTATGACGGAATCTAAGGTTTCAGCAAGTATCTGCTGGCTTGGTCATG -460 bp

Fig. S3. TG2 and AR protein expression in selected prostate tumor samples (A). TG2 expression Is associated with increased accumulation of HIF-1α protein in prostate cancer cells (B)

TG2 AR

HIF-1α

β-actin

Gene designation Primer Sequence (5’-3’)

AR F: TTGGATGGCTCCAAATCACR: GCAATGATACGATCG AGTTCC

E-cadherin F: TCCCATCAGCTGCCCAGA A A

R:TGACTCCTGTGTTCCTGT TA

N-cadherin F: ACAGTGGCCACCTACAAAGGR: CCGAGATGGGGTTGATAATG

Vimentin F: GAG AACTTTGCCGTTGAA GC

R: GCTTCCTGTAGGTGGCAATC

Fibronectin F: CAGTGGGAGACCTCGAGAAG R:TCCCTCGGAACATCAGAAAC

ZEB1 F: CTGAAGAGGACCAGAGGCAG

R: CCCAGAACTGCGTCACATGTC

ZEB2 F: AAGTACCGCCACGAGAAGAA

R: TTGTTTCTCATTCGGCCATT

Snail F: TTCTTCTGCGCTACTGCTGCGR:

GGGCAGGTATGGAGAGGAAGA

TG2 F: TATGGCCAGTGCTGGGTCTTCGCC R: GGCTCCAGGGTTAGGTTGAGCAGG

GAPDH F: ACCACAGTCCATGCCATCAC

R: TCCACCACCCTGTTGCTGTA

Table S1 – Primers used to amplify various transcripts

Fig. S1. Effect of TG2 expression on LNCaP cell growth (A) and

Documents

Transcript of Fig. S1. Effect of TG2 expression on LNCaP cell growth (A) and

Fig. 10.01

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