Additional File 2: Figure S2

Post on 29-Jan-2016

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Additional File 2: Figure S2. si BTG2( nM ). 0 25 50 100. siCont ( nM ). 100 75 50 0. S2A. S2B. I k B α. IB. MCF7 cells. MCF7 cells. kDa. Actin. IkB - α. 35. RT-PCR. BTG2. 63. α - Tubulin. GAPDH. Negative. ChIP : a -p65 - PowerPoint PPT Presentation

Transcript of Additional File 2: Figure S2

Additional File 2: Figure S2

kDa

pcDNA3-HA

BTG2-HA

Transfection

0.0 0.4 0.8 mg

0.8 0.4 0.0 mg

α-Tubulin63

BTG2-HA20

IkB-α35

MCF7 cellsS2A S2B

LacZ BTG2-HA

Input

Adenovirus(100 moi)

kB-RE

LacZ BTG2-HA IgG

ChIP: a-p65 antibody Negative

MEF cells

100 75 50 0

0 25 50 100

IkBα

Actin

BTG2

siCont (nM)

si BTG2(nM )

RT

-PC

RIB

GAPDH

S2C

MCF7 cells