TWF1 Polyclonal ANTIBODYCatalog Number:11732-1-AP Featured Product 1 Publications

For Research Use Only

www.ptglab.com

Basic Information Catalog Number:11732-1-AP

Size:150UL , Concentration: 133 μg/ml byBradford method using BSA as thestandard;

Source:Rabbit

Isotype:IgG

Immunogen Catalog Number:AG2332

GenBank Accession Number:BC022344

GeneID (NCBI):5756

Full Name:twinfilin, actin-binding protein,homolog 1 (Drosophila)

Calculated MW:368 aa, 42 kDa

Observed MW:42 kDa

Purification Method:Antigen affinity purification

Recommended Dilutions:WB 1:1000-1:6000 IHC 1:50-1:500

Applications Tested Applications:IHC, WB, ELISA

Cited Applications:IF, WB

Species Specificity:human, mouse, rat

Cited Species:human

Note-IHC: suggested antigen retrievalwith TE buffer pH 9.0; (*) Alternatively,antigen retrieval may be performedwith citrate buffer pH 6.0

Positive Controls:

WB : C2C12 cell,

IHC : human lung cancer tissue, human breast cancertissue

Background Information

Notable Publications Author Pubmed ID Journal Application

Lu Li 28864421 Biochem Biophys Res Commun WB,IF

Storage Storage:Store at -20°C. Stable for one year after shipment.Storage Buffer:PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Aliquoting is unnecessary for -20ºC storage

T: 1 (888) 4PTGLAB (1-888-478-4522) (toll freein USA), or 1(312) 455-8498 (outside USA)

E: proteintech@ptglab.com W: ptglab.com

For technical support and original validation data for this product please contact: This product is exclusively available under ProteintechGroup brand and is not available to purchase from anyother manufacturer.

Selected Validation Data

Various lysates were subjected to SDS PAGEfollowed by western blot with 11732-1-AP (TWF1antibody) at dilution of 1:3000 incubated at roomtemperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using11732-1-AP (TWF1 antibody) at dilution of 1:200(under 10x lens). Heat mediated antigen retrievalwith Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using11732-1-AP (TWF1 antibody) at dilution of 1:200(under 10x lens). Heat mediated antigen retrievalwith Tris-EDTA buffer (pH 9.0).