Dystrophin Polyclonal ANTIBODYCatalog Number:12715-1-AP Featured Product 4 Publications

For Research Use Only

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Basic Information Catalog Number:12715-1-AP

Size:150UL ,250 μg/mL

Source:Rabbit

Isotype:IgG

Purification Method:Antigen affinity purification

Immunogen Catalog Number:AG3408

GenBank Accession Number:BC028720

GeneID (NCBI):1756

Full Name:dystrophin

Calculated MW:3685aa,427 kDa

Observed MW:70 kDa

Recommended Dilutions:WB 1:500-1:3000 IP 0.5-4.0 ug for IP and 1:500-1:3000for WBIHC 1:50-1:500 IF 1:20-1:200

Applications Tested Applications:IF, IHC, IP, WB, ELISA

Cited Applications:IF, IP, WB

Species Specificity:human, mouse, rat

Cited Species:human, mouse, rat

Note-IHC: suggested antigen retrieval withTE buffer pH 9.0; (*) Alternatively, antigenretrieval may be performed with citratebuffer pH 6.0

Positive Controls:

WB : mouse brain tissue; human testis tissue, mouselung tissue

IP : mouse brain tissue;

IHC : human skeletal muscle tissue; human hearttissue

IF : mouse skeletal muscle tissue;

Background Information Dystrophin (DMD or BMD) is a large muscle protein whose mutations cause Duchenne muscular dystrophy (DMD) andBecker muscular dystrophy (BMD), the childhood neuromuscular disorders that result in progressive muscleweakness, respiratory difficulties and cardiovascular dysfunction. Dystrophin is a crucial component of thedystrophin-glycoprotein complex which is essential for muscle membrane integrity and stability. Dystrophin islocated on the cytoplasmic face of the sarcolemma and connects the cytoskeletal network to the sarcolemma andextracellular matrix. Multiple isoforms of dystrophin exist due to the alternative splicing, with a wide range of MW(69-72, 110-143, 271, 426 kDa). Most tissues contain transcripts of several isoforms.

Notable Publications Author Pubmed ID Journal Application

Wen-Tong Liu 30340642 J Neuroinflammation WB

Brent A Fujimoto 31593505 Am J Physiol Endocrinol Metab IF

I-Ling Lu 29950674 Nat Commun WB,IF

Storage Storage:Store at -20°C.Storage Buffer:PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Aliquoting is unnecessary for -20ºC storage

T: 1 (888) 4PTGLAB (1-888-478-4522) (toll freein USA), or 1(312) 455-8498 (outside USA)

E: proteintech@ptglab.com W: ptglab.com

For technical support and original validation data for this product please contact: This product is exclusively available under ProteintechGroup brand and is not available to purchase from anyother manufacturer.

Selected Validation Data

Immunofluorescent analysis of ( 4% PFA) fixedmouse skeletal muscle tissue using 12715-1-AP(Dystrophin antibody) at dilution of 1:50 and AlexaFluor 488-conjugated AffiniPure Goat Anti-RabbitIgG(H+L)

IF result of anti-DMD (12715-1-AP, 1:500) with PFAfixed mouse skeletal muscle tissue by Dr. DanielKopinke.

mouse brain tissue were subjected to SDS PAGEfollowed by western blot with 12715-1-AP(Dystrophin antibody) at dilution of 1:1500incubated at room temperature for 1.5 hours

IP Result of anti-Dystrophin (IP:12715-1-AP, 4ug;Detection:12715-1-AP 1:1500) with mouse braintissue lysate 3440ug.

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slideusing 12715-1-AP( Dystrophin antibody at dilutionof 1:200 (under 40x lens).

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slideusing 12715-1-AP( Dystrophin antibody at dilutionof 1:200 (under 10x lens).