Download - p-p53 (p18S)

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Page 1: p-p53 (p18S)

p-p53 (p18S)

p53

γH2AX (p140S)

H2AX

tubulin

5 Gy IR - - - - - - - -+ + + + + + + +

Atm+/+Wip1+/+ Atm+/+Wip1-/- Atm-/-Wip1+/+ Atm-/-Wip1-/-

Darlington_Supplemental Figure 1

Page 2: p-p53 (p18S)

5 Gy IR

Atm+/+

Wip1+/+Atm+/+

Wip1-/-Atm-/-

Wip1+/+Atm-/-

Wip1-/-

- - + + - - - - - -+ + + + + +p-Brca1

(p1423S)

Brca1

tubulin

Darlington_Supplemental Figure 2

Page 3: p-p53 (p18S)

0

20

40

60

80

100

no IR +IR no IR +IR no IR +IR no IR +IR

WT Wip-/- Atm-/- DKO

5 Gy IR

Atm+/+

Wip1+/+Atm+/+

Wip1-/-Atm-/-

Wip1+/+Atm-/-

Wip1-/-

- - ++ + + + + + +- - - - - -cleaved PARP1

cleaved caspase-3

tubulin

Cleaved PARP1 protein levels (normalized to tubulin)

Arb

itra

ry u

nit

Cleaved Caspase-3 protein levels (normalized to tubulin)

0

20

40

60

80

100

no IR +IR no IR +IR no IR +IR no IR +IR

WT Wip-/- Atm-/- DKO

Arb

itra

ry u

nit

A

B C

Atm+/+

Wip1+/+Atm+/+

Wip1+/+Atm+/+

Wip1-/-Atm+/+

Wip1-/-Atm-/-

Wip1+/+Atm-/-

Wip1+/+Atm-/-

Wip1-/-Atm-/-

Wip1-/-

5 Gy IR 5 Gy IR- - - - - - - -+ + + + + + + +

100 100

80 80

60 60

40 40

20 20

0 0

Darlington_Supplemental Figure 3