p-p53 (p18S)

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p-p53 (p18S) p53 γH2AX (p140S) H2AX tubuli n 5 Gy IR - - - - -- -- + + + + + + + + Atm +/+ Wip1 +/+ Atm +/+ Wip1 -/- Atm -/- Wip1 +/+ Atm -/- Wip1 -/- Darlington_Supplemental Figure 1

description

Darlington_Supplemental Figure 1. Atm -/- Wip1 +/+. Atm -/- Wip1 -/-. Atm +/+ Wip1 +/+. Atm +/+ Wip1 -/-. p-p53 (p18S). p53. γ H2AX (p140S). H2AX. tubulin. 5 Gy IR. -. -. -. -. -. -. -. +. +. +. -. +. +. +. +. +. Darlington_Supplemental Figure 2. Atm -/- Wip1 -/-. - PowerPoint PPT Presentation

Transcript of p-p53 (p18S)

Page 1: p-p53 (p18S)

p-p53 (p18S)

p53

γH2AX (p140S)

H2AX

tubulin

5 Gy IR - - - - - - - -+ + + + + + + +

Atm+/+Wip1+/+ Atm+/+Wip1-/- Atm-/-Wip1+/+ Atm-/-Wip1-/-

Darlington_Supplemental Figure 1

Page 2: p-p53 (p18S)

5 Gy IR

Atm+/+

Wip1+/+Atm+/+

Wip1-/-Atm-/-

Wip1+/+Atm-/-

Wip1-/-

- - + + - - - - - -+ + + + + +p-Brca1

(p1423S)

Brca1

tubulin

Darlington_Supplemental Figure 2

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no IR +IR no IR +IR no IR +IR no IR +IR

WT Wip-/- Atm-/- DKO

5 Gy IR

Atm+/+

Wip1+/+Atm+/+

Wip1-/-Atm-/-

Wip1+/+Atm-/-

Wip1-/-

- - ++ + + + + + +- - - - - -cleaved PARP1

cleaved caspase-3

tubulin

Cleaved PARP1 protein levels (normalized to tubulin)

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Cleaved Caspase-3 protein levels (normalized to tubulin)

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no IR +IR no IR +IR no IR +IR no IR +IR

WT Wip-/- Atm-/- DKO

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Darlington_Supplemental Figure 3