γλώσσες
Σελίδες
Νομικός
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
ITGA10
α
in vivo
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
NF1
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
β β β
β
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
in vitro in vivo
Gene expression profiles define two molecular subtypes of myxofibrosarcoma
p
p
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
p
p
p
Silencing of integrin- 10 induces growth suppression through apoptosis in
myxofibrosarcoma cells but not in normal mesenchymal cells
ITGA10
ITGA10
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
ITGA10
Forced expression of integrin- 10 enhances cell migration and invasion
Integrin- 10 regulates the activities of RAC/PAK and AKT in myxofibrosarcoma
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
TRIO and RICTOR are overexpressed via DNA amplification in myxofibrosarcoma and
physically associate with integrin- 10
TRIO RICTOR
TRIO
RICTOR
RICTOR
RICTOR TRIO
RICTOR TRIO
TRIO RICTOR
ITGA10
TRIO
TRIO RICTOR
TRIO RICTOR
TRIO RICTOR
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
TRIO and RAC are required for growth of myxofibrosarcoma cells
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
RICTOR is essential for activation of AKT and cell growth for myxofibrosarcoma cells,
but not ASCs
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
Constitutively active RAC, PAK, and AKT rescue ITGA10 knockdown-induced growth
suppression and apoptosis
.
Pharmacologic inhibitors of RAC and mTORC inhibit growth of myxofibrosarcoma cells
in vitro
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
mTOR inhibition sensitizes myxofibrosarcoma cells to RAC inhibitors
EHop-016 and INK128 suppress myxofibrosarcoma primary and metastatic tumor growth
in mouse xenografts
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
ITGA10
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
β β
Itga10
TRIO
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
in vitro
in vitro
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
in vivo
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
Patients
RNA isolation and microarray analysis
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
Statistical Analysis
ITGA10 TRIO RICTOR
Pathway and Gene Annotation Analysis
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
Copy Number Analysis
Cell Culture and Reagents
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
Lentiviral transduction for shRNA and gene expression
Cell proliferation and apoptosis
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
Migration and Matrigel invasion
Western Blotting
Biochemistry
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
Collagen adhesion
Mouse tumorigenicity and metastasis
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
ITGA10
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
α
α
α
t P
P P
TRIO RICTOR
TRIO RICTOR
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
ITGA10
TRIO RICTOR TRIO
RICTOR
α
α
α
α
ITGA10 α
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
in vitro
α
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
α
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
p=0.0497
p=0.029p=0.043
p=0.019
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
Published OnlineFirst August 30, 2016.Cancer Discov Tomoyo Okada, Ann Y. Lee, Li-Xuan Qin, et al. therapeutic targets in high-grade myxofibrosarcoma
10 dependency identifies RAC and RICTOR asαIntegrin-
Updated version
10.1158/2159-8290.CD-15-1481doi:
Access the most recent version of this article at:
Material
Supplementary
http://cancerdiscovery.aacrjournals.org/content/suppl/2016/08/30/2159-8290.CD-15-1481.DC1.htmlAccess the most recent supplemental material at:
Manuscript
Authoredited. Author manuscripts have been peer reviewed and accepted for publication but have not yet been
E-mail alerts related to this article or journal.Sign up to receive free email-alerts
Subscriptions
Reprints and
To order reprints of this article or to subscribe to the journal, contact the AACR Publications
Permissions
To request permission to re-use all or part of this article, contact the AACR Publications
Research. on September 12, 2016. © 2016 American Association for Cancercancerdiscovery.aacrjournals.org Downloaded from
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on August 30, 2016; DOI: 10.1158/2159-8290.CD-15-1481
Top Related