Rating:

Specificity:

ANTIBODY CUSTOMER REVIEWFindings: “Single specific band at the correct molecular weight.” – Benjamin Clarke, UCL.

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Protocol

Gel Electrophoresis

10% polyacrylamide gel, constant voltage 160 V, 60 min.

Membrane Wash

1X TBST wash for 3 times.

Transfer 0.45 nm nitrocellulose membrane, 1 piece of gel (wet transfer), constant voltage 100 V 60 min.

Secondary Antibody

Probing

Secondary antibody was added to the solution of 1X TBST with 5% BSA with 1:10000 dilution ratio, for 2 hr.

Blocking 1X TBST with 5% Bovine serum albumin (BSA) for 2 hr.

Membrane Wash

1X TBST wash for 3 times.

Primary Antibody

Probing

Primary antibody was added to the solution of 1X TBST with 5% BSA, 4˚C for overnight.

Visualisation ECL visualization using Luminata Crescendo.

Model Number STJ97037

Antibody Name Anti-β-tubulin antibody

Application Western Blot

Species Human

Tissue Primary cortical astrocytes

Dilution 1:1000

Secondary Antibody Dako swine anti-rabbit P0217

Secondary Dilution 1:2500

Reference Antibody Actin (abcam) ab6276

Fig. In primary astrocytes, target bands were faintly detected by STJ97037. TUJ1 antibody (Figure: Lane1: Control primary cortical astrocytes; Lane 2: Heat shock primary cortical astrocytes).

B actin loading

control