GENESDEV/2009/121111; FigureS1; Jechlinger et...

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A B γ-Tubulin β-Tubulin β-Tubulin ZO1 Jechlinger_Supplementary Fig. 1 GENESDEV/2009/121111; FigureS1; Jechlinger et al.

Transcript of GENESDEV/2009/121111; FigureS1; Jechlinger et...

Page 1: GENESDEV/2009/121111; FigureS1; Jechlinger et al.genesdev.cshlp.org/content/suppl/2009/07/01/23.14.1677.DC1/Jechli… · A B γ-Tubulinβ-Tubulin β-Tubulin ZO1 Jechlinger_Supplementary

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γ-Tubulinβ-Tubulin

β-Tubulin ZO1

Jechlinger_Supplementary Fig. 1

GENESDEV/2009/121111; FigureS1; Jechlinger et al.

Page 2: GENESDEV/2009/121111; FigureS1; Jechlinger et al.genesdev.cshlp.org/content/suppl/2009/07/01/23.14.1677.DC1/Jechli… · A B γ-Tubulinβ-Tubulin β-Tubulin ZO1 Jechlinger_Supplementary

Supplementary Figure 1 Primary mammary cells orient their mitotic spindle

perpendicular to an apical surface and form a lumen without involvement of

apoptotic mechanisms

(A) Orientation of cell-division in an expanding polarized acinus that is grown from

primary non-transgenic mammary cells. One cell in anaphase is dividing with the two

sets of chromosomes perpendicular to the lumen, which can be appreciated in the bottom

panels; 6 adjacent projections (4µm each) cover the region of the sphere (from upper left

panel to lower right panel); DAPI stain, blue; β-tubulin, green; γ-tubulin, red. Scale bar,

20µm (B) Another example for the orientation of cell-division in an expanding polarized

acinus that is grown from primary non-transgenic mammary cells. One cell in anaphase is

dividing with the two sets of chromosomes perpendicular to the lumen (right panels). The

front part of the acinus has been cut in the 3D reconstruction to better appreciate the ZO1

lined lumen. Panels display cuts with a second optical layer at different levels (left panel:

lower level; middle panels: intermediate levels; right panel: whole acinus) to appreciate

the lumen and the orientation of the cell in anaphase that is located at the top of the

structure. DAPI stain, blue; ZO1, green; beta-tubulin, red. Scale bar, 20µm.