SHORT CURICULLUM VITAE Μ.Α.ΤΥPΑS, 2015

SHORT CURICULLUM VITAE

Μilton A. Τypas

2015

POSITIONS HELD

1975-1977 Post-Doctoral and Temporar Lecturer, University of London (Queen Elizabeth College)

1978-1980 Lecturer, Athens University 1978-1980

1980-1986 Tenured Lecturer at Athens University

1986-1992 Senior Lecturer

1992-2000 Reader

Jan2001- Professor, Head of the Department 1997-98, 1999-2000, 2002-2015.

VISITING PROFESSOR 1989-1990 Visiting Professor, King’s College, London University 1989-1990 (one year, sabbatical

leave of absence).

1990 Visiting Researcher, Julich, Kernforschungsanlage, Germany 1990 (3 months)

2000 Visiting Professor, Novartis Research Centre (NIMRI) – U.C. San Diego (4 months,

sabbatical leave of absence).

2001-2002 Visiting Professor, Cornell University, NY, 2001-2002 (14 months; sabbatical leave of

absence).

1981-2000 Visiting-collaborating researcher with various Universities-Institutes in Europe

(Belgium, France, Germany, Netherlands and UK) for short periods at summers

(total around 6 months).

SCHOLARSHIPS 1970-1971 Research Centre Democritus 1970-71 (undergraduate research assistantship), Αthens, GR

1972-1975 London University, QEC, UK, 1972-1975 (Ph.D. demonstratorship)

1975-1977 London University and Agricultural Research Council, UK, Postdoctoral and Temporar

Lecturer

1977 Royal Society of Britain

1978, 1980,

1981, 1982

Junior Researcher, British Council, QEC, UK, (summer 3 month grants)

1983, 1985 EMBO, and EU mobility grants, London University, KQC, UK and Julich, Germany

(summer 3 month grants)

1990, 1994 British Council, KQC, UK (9 months and 2 months; Senior Researcher)

FUNDED RESEARCH PROJECTS

EU

1984-1986

Biotechnology Engineering Programme [BEP-DGXII-EC].

1986-1989 Biotechnology Action Programme [BAP-DGXII-EC].

1990-1993 Biotechnology [BIOTECH- DGXII-EC].

1994-1996 Human Capital Mobility. Insect pathogenic fungi for economic, environmentally friendly

pest control in the glasshouse (AIR3-CT94-1352)

1995-1998 AIR3-CT94-1352. Biocontrol of important soil dwelling pests by improving the efficacy

of insect pathogenic Fungi [ΒIPESCO].

1998-2001 FAIR6-CΤ98-4105. Determining molecular markers for the genetic analysis of

recombinant strains of entomopathogenic fungi used for biological control.

2002-2005

2005-2008

2010-2012

QLK-CT-2001-01391: Risk Assessment of Biological Control Agents [RAFBCA].

FP6-2004-SSP-4: Specific Support Action, Regulation of Biocontrol Agents [REBECA]

Molecular identification nd characterization of Beauveria bassiana strains INRA 147,

ATCC4040, BELCHIM BB1, NPP-111B005 and Laverlam.

SHORT CURICULLUM VITAE Μ.Α.ΤΥPΑS, 2015

National-EU

1986-1988 ΠΡΟΠΕ The genetics and physiology of Zymomonos mobilis

1989-1992 ΠΕΝΕΔ Production of bioethanol by the entanologenic bacterium Zymomonas mobilis.

1995-1998 EΠET ΙΙ An integrated and novel approach of producing added value products (amino

acids and oligosaccharides from by-products of sugar producing factories.

1996-1998 ΠΕΝΕΔ II Genetic fingerprinting and phylogenetic relationships of plant pathogenic and

non-pathogenic species of the genus Verticillium based on molecular, genetic and

immunogenetic techniques.

1997-1998

1997-1999

1998-1999

ΠΑΒΕ Reduction of losses in sugar production by controlling microbial activities in the lines of of

Industrial production.

Joint Research and Technology Projects: Franco-Greek binational Collaboration on

«Determining molecular markers for the genetic identification and analysis of genetic

recombinant strains of the entomopathogenic fungi Verticillium lecanii and Beauveria

bassiana that are used as biocontrol agents».

GREEK-RUSSIAN BINATIONAL COLLABORATION: An integrated approach to the

identification of species and subspecies grouping in the genus Verticillium.

1998-2001

1999-2002

1999-2002

2002-2004

EΠET ΙΙ The alcoholic fermentation during wine production: Screening and assessment of πιλογή –

yeasts, study of fermentation parameters and quality improvement of wines produced.

ΠΕΝΕΔ 99 “Μοlecular mechanisms of bacterial resistance to variable sugar concentrations

–industrial lines- in microorganisms of industrial interest.

ΕΠΕΤΙΙ- BIOPRO- 98GE2, “Improved technologies to increase resistance of plants to

bacterial and fungal pathogens by genetic engineering, non invasive genetic approaches

and other –non chemical- strategies.‘Construction and development of a comprehensive

interactive database for the analysis of fungal mitochondrial genomes”

HUMAN NETWORKS, ‘Biotechnological and bio-ethical approaches towards employing

microorganisms for industrial, clinical, agricultural and environmental applications’.

2003-2006 ΕΚΒΑΝ-ΕΠΕΤΙΙ-ΤΡ5: ‘An integrated strategy for the safety of Dairy Product Industry from

microbial contamination: use of fast, modern and accurate methods for the detection and

genetic identification of spoiling or pathogenic microorganisms – development of model

examination plan for the everyday handling in the production lines’.

2004-2007

ΕΚΒΑΝ-ΕΠΕΤΙΙ- ΦΠ66 ‘Βiological treatment and exploitation of Olive oil Mill Waste-

water: Μechanisms and integrated applicatios’.

2011-2014 ΗERAKLEITOS ΙΙ: Genetic and molecular study of heterokaryosis in the plant pathogenic

fungi of the genus Verticillium.

2013-2015 ΤΗΑLIS MIS 63.0001 ‘Βiological Investigation Of the Forces that Influence the Life of

Pathogens having as Mission to Survive in various Lifestyles [BIOFILMS]

2013-2015 THALIS MIS 377062 ‘‘Metagenomics of ligninolytic microorganisms – Bioconversion of

plant by-products into high-added value products ’’[LIGNOMET]. Coordinator

2013-2015 COOPERATION 2011 ‘‘The Sustainable Integrated Method for the Production of

Lignocellulosic Ethanol’’ [SIMPLE].

ΑDMINISTRATIVE ACTIVITIES

1982-1996 Executive member of the National Hellenic Research Foundation 1982-1996

(6 consecutive terms). 1982-1988 Director of the National Documentation Centre, National Hellenic Research

Foundation (ΕΚΤ),

Executive member and Vice-Chairman of its Scientific Committee 1989-1999.

1982-1987 National Delegate for Science and Technology, U.N.

1983-1986 National Representative for the Committee for Science and Technology Programme, EC.

1986-2005 Member of Advisory Committees for Science and Technology (CCE) in EU, and

Expert of National Delegations in Science & Research, Biotechnology, Agriculture

SHORT CURICULLUM VITAE Μ.Α.ΤΥPΑS, 2015

and Energy DG’s VI, XII and ΧΙΙI, EU.

1986-2010 Independent expert/evaluator of research proposals and grant applications of young

investigators in the EU (more than 200 proposals and 300 applications, respectively, in

subjects of Βiotechnology, Molecular-Microbial Genetics, Βiodiversity, Biosafety, Plant

Pathology, Plant-microbe interactions, Biocontrol, Biomass, Bioethanol etc) for various

Programmes [e.g. ΒΙΟΤΕCH, FAIR, COST, INTAS, COPERNICUS, THIRD

COUNTRIES, STP, MARIE-CURRIE, FP5, FP6, FP7].

1996-1998 Member of the National Advisory Committee for Research (ΕΓΣΕ).

2001-2004

1996-2004

Member of the National Council for Research and Technology (ΕΣΕΤ).

Chairman Special Advisory Committee in Biotechnology. 1982-2014 Reviewer of research manuscripts for more than 50 International ICI-Indexed

Journals and member of the editorial board in three Journals. Has reviewed more than

600 manuscripts. Also, the reviewer of 14 book chapters.

2007-2010 Executive Member of the Research Committee of the National and Kapodistrian University of

Athens.

2010-2014 Full member of the Executive Research Committee of the National and Kapodistrian

University of Athens. .Τακτικό μέλος της Eπταμελούς Επιτροπής Ερευνών E.K.Π.Α.

1997-2004 Μember of the National Competent Authority for the control of genetically engineered

organisms and products.

1996-2010

1995-1997

2003-2015

Ad hoc expert-advisor for GMO organisms/products in EU Committees.

Vice-Chairman of the Faculty of Biology, University of Athens Head of the Department of Genetics and Biotechnology

Member of electoral bodies for Faculty member evaluations in all Greek Universities (more

than 70 elections during the past decade)

Member of electoral bodies for all the Research Institutes of Greece (over 20 elections of

directors and researchers of Institutes during the past years).

RESEARCH ACTIVITIES/EXPERIENCE

Head of the Microbial-Molecular Genetics and Biotechnology group. Research interests are divided

into two major lines:

a) Fungal Genetics / Biotechnology: Molecular typing/genetic fingerprinting of fungi with economic

importance (phytopathogenic, entomopathogenic, yeasts), based on standard molecular techniques

(RFLPs, RAPD, AFLP, DGGE, PFGE, PCR, RT-PCR, DNA/DNA, DNA/RNA hybridizations,

immunolabelling, etc). Analysis of completed fungal mitochondrial genomes, gene structure-function,

and phylogenetic relationships (an interactive database –MitoFun- to appear shortly at the net). Host-

parasite relationships with model entomopathogens and phytopathogens. Study of the molecular

mechanisms of heterokaryosis in mitosporic fungi. Phylogenetic analysis/implications, biodiversity and

biosafety. Isolation, cloning and characterization of basidiomycetes genes involved in wood

decomposition and assessment of their potential in ethanol production. Genomic and metagenomic

approaches for the discovery and isolation of novel genes/gene products involved in cellulose and

ligninocellulose degradation.

More classical approaches like mutagenesis, mutant production/characterization, mitotic recombination

analyses, protoplast regeneration, protoplast fusion, transformation of cloning/expression vectors, gene

isolation/characterization, gene-overexpression and the exploitation of parasexual cycle are also

routinely used. Construction of knock-out mutants by gene-replacement/gene-inactivation methods are

in use for fungi of interest.

b) Bacterial Genetics / Βiotechnology: Various bacteria are used for the production of enzymes, fine

chemicals and polysaccharides of industrial importance. Chemical and transposon mutagenesis,

isolation and characterization of plasmids, transposable elements, isolation-cloning-characterization of

genes, strain construction, construction of suitable cloning and expression vectors etc. are common lab

practices. Particular emphasis is placed on the genetics if the ethanol producing bacterium Zymomonas

mobilis, which is used as a model organism. Collaborations with the JGI/DOE of USA (PI, Dr K-

M.Pappas) resulted in the complete sequencing and annotation of the genomes of 6 different

SHORT CURICULLUM VITAE Μ.Α.ΤΥPΑS, 2015

Zymomonas strains and the transcriptome analysis of the most important strain for ethanol production

under various conditions is pending. Genetic engineering of strains of the bacterium for the production

of bioethanol from cellulosic-ligninocellulosic residues as well as of value added chemicals is under the

way in collaboration with Ass.Prof. K-M.Pappas.

The lab is equipped with all necessary instruments to contact experiments in Molecular-Microbial

Genetics and Biotechnology.

Apart from the collaboration with the Assistant Professor Dr.K-M.Pappas and her research group,

collaboration includes the Lecturer Dr.V.N.Kouvelis, the PostDocs Dr.I.Papaioannou, Dr.G.Efthymiou,

and the Ph.D. students- researchers M.Kalntremtziou, V.Diakogiannaki, V.Vaggalis.

TEACHING AND Ph.D. SUPERVISOR/EXAMINER

The co-ordinator of three under-graduate courses: (a) Basic Genetics (compulsory), (b) Advance

Genetics (choice course), (c) Biotechnology (choice course), and one post-graduate course for Ph.D.

students, in Advanced Molecular Genetics. Also teaching –lectures- in 3 different M.Sc. courses.

Supervisor of 40 Ph.D. theses (22 directly his own students who have graduated, and 18 from Research

Institutes, as acting supervisor according to Greek, all of whom have graduated).

Member of the examination board for more than 200 Ph.D. theses in Greece and 16 abroad (U.K.4,

France 3, Spain 2, USA 2, and 1 in each of Germany, Canada, India and Pakistan).

CONFERENCES, MEETINGS AND SYMPOSIA

President of Organizing Committees in 3 International and 12 National Conferences/Symposia.

Organizing Committee Member and Chair in 51 occasions, invited speaker (50 International and 40

National Conferences/Meetings), with more than 220 published presentations, abstracts and posters.

Invited speaker on ‘popularized Biology science’ at Town Halls and Open Universities. Speaker on

GMOs and participation at round table discussions in tens of cases during the period 1995-2008.

OTHER ACTIVITIES

Has deposited more than 1,000 different sequences/packets of sequences in public domains like the

NCIB Gene Bank. Some of the most important are: (a) the complete genomes of 6 bacteria, 12 fungal

mitochondrial DNAs and 18 large plasmids, and (b) packets in population studies, Verticillium (252);

Metarhizium (234); Lecanicillium (230); Beauveria (190); Candida zemplinina (28); Zymomonas

mobilis (50); the yet unreleased Aspergillus (224) ; Penicillium (360), Pleurotus (416) and many single

entries.

Author of books (2) and booklets (6) for University underground students, 22 chapters in books ans

collective volumes [some of the more recent: “Verticillium”. In “Molecular detection of human fungal

pathogens”, ed. Liu D, JF Taylor & Francis CRC Press, Chapter 63, p.527-540 (2011); “Phylogenetic

analysis of entomopathogenic fungi”. In “Microbial Insecticides: Principles and applications” ed.

Borgio JF, Schavaraj H & Susurluk A, NOVA Science Publishers, (2011); “Assessing genotoxic effects

of microbial products", in "Microbes and the Law - Safety Assessment and Regulation of Beneficial

Microorganisms", Chapter 7, Editors: Ingvar Sundh, Andrea Wilcks & Mark S. Goettel, CABI

Publishing (2012); “A Phylogenetic analysis of Greek isolates of Aspergillus species based on

morphology, nuclear and mitochondrial gene sequences” in Microbial Diversity for Biotechnology, ed.

Hindawi Publications (2013)].

].

SHORT CURICULLUM VITAE Μ.Α.ΤΥPΑS, 2015

MOST RECENT PUBLICATIONS (LAST 10 YEARS)

1. Wang C, Typas MA and Butt TM (2005). Phylogenetic and exon-intron structure analysis of fungal

subtilisins : Support for a mixed model of intron evolution. Journal of Molecular Evolution 60: 238-246.

2. Pantou M and Typas MA (2005). Electrophoretic karyotype and gene mapping of the vascular wilt

fungus Verticillium dahliae. FEMS Microbiology Letters 245: 213-220.

3. Pantou MP, Strunnikova OK, Shakhnazarova VYu, Cishnevskaya NA, Papalouka VG and Typas MA

(2005). Molecular and immunochemical phylogeny of Verticillium species. Mycological Research 109:

889-902.

4. Pramateftaki P, Lanaridis P, Kouvelis VN and Typas MA (2006). The mitochondrial genome of the

wine yeast Hanseniaspora uvarum : a unique genome organization among yeast/fungal counterparts.

FEMS Yeast Research 6: 77-90.

5. Ghikas D, Kouvelis VN and Typas (2006). The complete mitochondrial genome of the

entomopathogenic fungus Metarhizium anisopliae var. anisopliae : gene order and trn clusters revealed

a common evolutionary course for all Sordariomycetes. Archives for Microbiology 185: 393-401

6. Pantou MP, Kouvelis VN and Typas (2006). The complete mitochondrial genome of the vascular wilt

fungus Verticillium dahliae: a novel gene order for Verticillium and a diagnostic tool for species

identification. Current Genetics 50: 125-136.

7. Pramateftaki P, Kouvelis VN, Lanaridis P and Typas MA (2008). The complete mitochondrial genome

sequence of the wine yeast Candida zemplinina: intra-species distribution of a novel group-IIB1 intron

with eubacterial affiliations. FEMS Yeast Research. 8: 311-327.

8. Kouvelis VN, Ghikas D, Edgington S, Typas MA and Moore D (2008). Molecular characterisation of

isolates of Beauveria bassiana obtained from over-wintering and summer populations of Sunn Pests

(Eurygaster integriceps). Letters in Applied Microbiology 46: 414-420.

9. Georgopoulos A, Typas MA and Demetzos K (2008). The use of liposomes as biosensors. An overview.

Pharmakeftiki 21: 22-29.

10. Kouvelis VN, Sialakouma A and Typas MA (2008). Mitochondrial gene sequences alone or combined

with ITS region sequences provide firm molecular criteria for the classification of Lecanicillium species.

Mycological Research 112: 829-844.

11. Pantou MP, Kouvelis VN and Typas MA (2008). The complete mitochondrial genome of Fusarium

oxysporum: insights into fungal mitochondrial evolution. Gene 419: 7-15.

12. Kouvelis VN, Saunders E, Brettin TS, Bruce D, Detter C, Han C, Typas MA and Pappas KM (2009).

Complete genome sequence of ethanol producer Zymomonas mobilis NCIMB 11163. Journal of

Bacteriology 191: 7140-7041. Epub 2009 Sep 18.

13. Yang S, Pappas KM, Hauser LJ, Land ML, Chen G-L, Hurst GB, Pan C, Kouvelis V, Typas MA,

Pelletier DA, Klingeman DM, Chang Y-J, Samatova NF and Brown SD (2009). Improved genome

annotation for Zymomonas mobilis. Nature Biotechnology 27: 893-894 (+additional material).

14. Ghikas DV, Kouvelis VN and Typas MA (2010). Phylogenetic and biogeographic implications inferred

by mitochondrial intergenic region analyses and ITS1-5.8S-ITS2 of the entomopathogenic fungi

Beauveria bassiana and B. brongniartii. BMC Microbiology 10:174 doi:10.1186/1471-2180-10-174.

15. Goudopoulou A, Krimitzas A and Typas MA (2010). Differential gene expression of ligninocellulolytic

enzymes in Pleurotus ostreatus grown on olive-oil mill wastewater. Applied Microbiology and

Biotechnology 88: 541-551. Doi:10.1007/s00253-010-2750-9.

16. Kouvelis VN, Pappas K-M, Wang C, Skrobek

A, Typas MA and Butt TM (2011). Assessing the

cytotoxic and mutagenic effects of secondary metabolites produced by several fungal biological control

agents with the Ames assay and the VITOTOX test. Mutation Research - Genetic Toxicology and

Environmental Mutagenesis 722: 1-6.

SHORT CURICULLUM VITAE Μ.Α.ΤΥPΑS, 2015

17. Pappas KM, Kouvelis VN, Saunders E, Brettin TS, Bruce D, Detter C, Balakireva M, Han C, Savvakis

J, Kyrpides NC and Typas MA (2011). Genome sequence of the ethanol-producing Zymomonas mobilis

subsp. mobilis lectotype ATCC 10988. Journal of Bacteriology 193: 5051-5052, doi:10.1128/JB.05395-

11.

18. Kouvelis VN, Davenport KW, Brettin TS, Bruce D, Detter C, Han C, Nolan M, Tapia R, Damoulaki A,

Kyrpides NC, Typas MA and Pappas KM (2011). Genome sequence of the ethanol-producing

Zymomonas mobilis subsp. pomaceae lectotype ATCC 29192. Journal of Bacteriology 193: 5049-

5050, doi:10.1128/JB.05273-11.

19. Typas MA and Kouvelis VN (2011). Phylogenetic analysis of entomopathogenic fungi. In “Microbial

Insecticides: Principles and applications”. ed. Borgio JF, Sahayaraj K and Susurluk IA, NOVA Science

Publications, Inc., NY., chapter 7, p.121- 148.

20. Pantou MP and Typas MA (2011). Verticillium, In “Molecular detection of human fungal pathogens”.

ed. Liu D, JF Taylor & Francis CRC Press, chapter 63, p.527-540.

21. Typas MA, Pantou MP and Kouvelis VN (2011). MtDNA and rDNA: Two different evolutionary lines

combined for genetic differentiation, taxonomy and phylogenesis in ascomycetes. XVI Congress of

European Mycologists, Chalkidiki, 19--23 Sept. 2011, pp.170-188.

22. Desiniotis A., Kouvelis VN, Davenport K, Bruce D, Detter C, Tapia R, Han C, Goodwin LA, Woyke T,

Kyrpides NC, Typas MA and Pappas KM (2012). The complete sequence of the ethanol-producing

Zymomonas mobilis subsp. mobilis centrotype ATCC 29191. Journal of Bacteriology 194: 5966-67,

DOI: 10.1128/JB.01398-12.

23. Typas MA and Kouvelis VN (2012). Assessing genotoxic effects of microbial products. In ‘’Beneficial

microorganisms in agriculture, food and the environment: safety assessment and regulation’’, ed. Sundh

I, Wilcks A & Goettel M, CABI Publ., chapter 18, pp. 256-274, DOI 10.1079/9781845938109.0256.

24. Ligoxigakis EK, Papaioannou IA, Markakis EA and Typas MA (2013). First report of pink rot of

Phoenix and Washingtonia palm species caused by Nalanthamala vermoesenii in Greece. Plant Disease

97 (2): 285. (dx.doi.org/10.1094/PDIS-08-12-0725-PDN)

25. Ligoxigakis EK, Markakis EA, Papaioannou IA and Typas MA (2013). First report of palm rot disease

of Phoenix spp. caused by Neodeightonia phoenicum in Greece. Plant Disease 97 (2): 286.

(dx.doi.org/10.1094/PDIS-08-12-0727-PDN)

26. Papaioannou IA, Ligoxigakis EK, Vakalounakis DJ, Markakis EA and Typas MA (2013)

Phytopathogenic, morphological, genetic and molecular characterization of a Verticillium dahliae

population from Crete, Greece. European Journal of Plant Pathology 136: 577-596, (DOI:

10.1007/s10658-013-0189-4).

27. Ligoxigakis EK, Papaioannou IA, Markakis EA and Typas MA (2013). First report of leaf spot of

Phoenix theophrasti caused by Paraconiothyrium variabile in Greece. Plant Disease 97 (9): 1250. (doi:

dx.doi.org/10.1094/PDIS-01-13-0114-PDN)

28. Krimitzas A, Pyrri I, Kouvelis VN, Kapsanaki-Gotsi E and Typas MA (2013). A Phylogenetic analysis

of Greek isolates of Aspergillus species based on morphology, nuclear and mitochondrial gene

sequences. Journal of Biomedicine and Biotechnology Article ID 260395, 18 pages. (in Microbial

Diversity for Bioetchnology, ed. Tsiamis G, Karpouzas D, Scherif A, Mavrommatis K, Hindawi Publ.)

29. Papaioannou IA, Dimopoulou Ch. and Typas MA (2013). Analysis of the intergenic region of the

nuclear ribosomal complex of Verticillium dahliae: a molecular tool for discrimination and phylogenetic

study of vegetative incompatibility groups. FEMS Microbiology Letters 347 (1): 23-32.

30. Ligoxigakis EK, Markakis EA, Papaioannou IA and Typas MA (2013). First Report of Petiole (Rachis)

Blight of Washingtonia filifera Caused by Phoma glomerata in Greece. Plant Disease 97 (11): 1509-

1510. (doi.org/10.1094/PDIS-04-13-0383-PDN)

SHORT CURICULLUM VITAE Μ.Α.ΤΥPΑS, 2015

31. Eboigbe L, Tzima AK, Paplomatas EJ and Typas MA (2014). Investigating the role of a β-

endoglucanase gene, vegB, in physiology and virulence of Verticillium dahliae. Phytopathologia

Mediterrenea 53 (1): 94-107. (http://dx.doi.org/10.14601/Phytopathol_Mediterr-13235).

32. Kouvelis VN, Teshima H, Bruce D, Detter C, Tapia R, Han C, Tampakopoulou V-O, Goodwin T,

Woyke T, Kyrpides N, Typas MA, Pappas KM (2014). Finished genome of Zymomonas mobilis subsp.

mobilis strain CP4, an applied ethanol producer. Journal of Bacteriology January/February 2014 vol. 2,

no. 1, e00845-13.

33. Papaioannou IA and Typas MA (2014). ‘Cryptic’ group-I introns in the nuclear SSU-rRNA gene of

Verticillium dahliae. Current Genetics 60: 135-148. DOI 10.1007/s00294-013-0417-7.

34. Zervakis, GI, Ntougias S, Gargano ML, Besi

MI, Polemis

E, Typas MA and Venturella G (2014). A new

Pleurotus species from Europe and reappraisal of the P. eryngii species-complex based on a polyphasic

approach, with a key to taxa associated to Apiaceae host-plants. Fungal Biology 118: 814-834. doi:

10.1016/j.funbio.2014.07.001.

35. Ligoxigakis EK, Markakis EA, Papaioannou IA and Typas MA (2015). First Report of Powdery

Mildew of Platanus × acerifolia and P. occidentalis Caused by Erysiphe platani in Greece. Plant

Disease 99 (2). 286 (dx.doi.org/10.1094/PDIS-07-14-0713-PDN).

36. Papaioannou IA and Typas MA (2015). Barrage zone formation is independent from vegetative

incompatibility in Verticillium dahliae. European Journal of Plant Pathology 141: 71-82.

doi: 10.1007/s10658-014-0525-3.

37. Papaioannou IA and Typas MA (2015). Vegetative compatibility in Verticillium dahliae: high-

throughput assessment and genetic aspects. Journal of Phytopathology 163: 475-485.

DOI: 10.1111/jph.12345.