SANTA CRUZ BIOTECHNOLOGY, INC.

OPN (AKm2A1): sc-21742

Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com

BACKGROUND

Osteopontin (OPN, also designated bone sialoprotein 1, urinary stone protein,spp-1, η-1, nephropontin, uropontin) is an extracellular matrix cell adhesionphosphoglycoprotein. OPN is deposited into unmineralized matrix prior tocalcification leading to localization at various tissue interfaces includingcement lines, lamina limitans, and between collagen fibrils of fully maturedhard tissues. While OPN is a major product of osteoblasts, it is also synthe-sized by brain and kidney cells. OPNs isolated from or secreted by varioustissues have molecular weights due to post-translational modifications. OPNfunctions as a substrate for transglutaminase and is involved in cell adhesion,chemoattraction and immunomodulation.

CHROMOSOMAL LOCATION

Genetic locus: SPP1 (human) mapping to 4q22.1; Spp1 (mouse) mappingto 5 E5.

SOURCE

OPN (AKm2A1) is a mouse monoclonal antibody raised against recombinantOPN of mouse origin.

PRODUCT

Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1%sodium azide and 0.1% gelatin.

OPN (AKm2A1) is available conjugated to agarose (sc-21742 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; to HRP (sc-21742 HRP), 200 µg/ml, forWB, IHC(P) and ELISA; to either phycoerythrin (sc-21742 PE), fluorescein(sc-21742 FITC), Alexa Fluor® 488 (sc-21742 AF488), Alexa Fluor® 546(sc-21742 AF546), Alexa Fluor® 594 (sc-21742 AF594) or Alexa Fluor® 647(sc-21742 AF647), 200 µg/ml, for WB (RGB), IF, IHC(P) and FCM; and to eitherAlexa Fluor® 680 (sc-21742 AF680) or Alexa Fluor® 790 (sc-21742 AF790),200 µg/ml, for Near-Infrared (NIR) WB, IF and FCM.

Alexa Fluor® is a trademark of Molecular Probes, Inc., Oregon, USA

APPLICATIONS

OPN (AKm2A1) is recommended for detection of OPN of mouse, rat andhuman origin by Western Blotting (starting dilution 1:200, dilution range1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total protein(1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilutionrange 1:50-1:500) and immunohistochemistry (including paraffin-embeddedsections) (starting dilution 1:50, dilution range 1:50-1:500).

Suitable for use as control antibody for OPN siRNA (h): sc-36129, OPNsiRNA (m): sc-36130, OPN shRNA Plasmid (h): sc-36129-SH, OPN shRNAPlasmid (m): sc-36130-SH, OPN shRNA (h) Lentiviral Particles: sc-36129-Vand OPN shRNA (m) Lentiviral Particles: sc-36130-V.

Molecular Weight of OPN precursor: 66 kDa.

Molecular Weight of OPN cleavage product: 25-55 kDa.

Positive Controls: OPN (m): 293T Lysate: sc-122246, Caki-1 cell lysate: sc-2224or U-87 MG cell lysate: sc-2411.

STORAGE

Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date ofshipment. Non-hazardous. No MSDS required.

DATA

SELECT PRODUCT CITATIONS

1. Wilson, A., et al. 2004. c-Myc controls the balance between hematopoieticstem cell self-renewal and differentiation. Genes Dev. 18: 2747-2763.

2. Saleh, S., et al. 2016. Osteopontin regulates proliferation, apoptosis,and migration of murine claudin-low mammary tumor cells. BMC Cancer16: 359.

3. Linh, N.T., et al. 2016. Augmenting in vitro osteogenesis of a glycine-arginine-glycine-aspartic-conjugated oxidized alginate-gelatin-biphasiccalcium phosphate hydrogel composite and in vivo bone biogenesisthrough stem cell delivery. J. Biomater. Appl. 31: 661-673.

4. He, Z., et al. 2016. Osteopontin and integrin αvβ3 expression during theimplantation window in IVF patients with elevated serum progesteroneand oestradiol level. Geburtshilfe Frauenheilkd. 76: 709-717.

5. Gauer, S., et al. 2016. Kidney injury molecule-1 is specifically expressedin cystically-transformed proximal tubules of the PKD/Mhm (cy/+) ratmodel of polycystic kidney disease. Int. J. Mol. Sci. E-published.

6. Cheng, G.S., et al. 2017. Bone marrow-derived mesenchymal stem cellsmodified with IGFBP-3 inhibit the proliferation of pulmonary artery smoothmuscle cells. Int. J. Mol. Med. 39: 223-230.

7. Ozeki, N., et al. 2017. MicroRNA-211 and autophagy-related gene 14signaling regulate osteoblast-like cell differentiation of human inducedpluripotent stem cells. Exp. Cell Res. 352: 63-74.

8. Song, M., et al. 2017. Aluminum trichloride inhibits the rat osteoblastsmineralization in vitro. Biol. Trace Elem. Res. 175: 186-193.

RESEARCH USE

For research use only, not for use in diagnostic procedures.

OPN (AKm2A1): sc-21742. Immunoperoxidase stainingof formalin fixed, paraffin-embedded human kidneytissue showing cytoplasmic staining of cells inglomeruli and cytoplasmic and faint nuclear stainingof cells in tubules (A) and human gall bladder tissueshowing cytoplasmic and faint nuclear staining ofglandular cells (B).

BA

OPN (AKm2A1): sc-21742. Western blot analysis ofOPN expression in non-transfected: sc-117752 (A) andmouse OPN transfected: sc-122246 (B) 293T wholecell lysates.

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