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Purification and Biochemical Characterization of a Specific β-Glucosidase fromthe Digestive Fluid of Larvae of the Palm Weevil, Rhynchophorus palmarumAuthor(s): Désiré Yapi Assoi Yapi, Dago Gnakri, Sebastien Lamine Niamke and Lucien Patrice KouameSource: Journal of Insect Science, 9(4):1-13. 2009.Published By: Entomological Society of AmericaDOI: http://dx.doi.org/10.1673/031.009.0401URL: http://www.bioone.org/doi/full/10.1673/031.009.0401

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Purification and biochemical characterization of a specific -glucosidase from the digestive fluid of larvae of the palmweevil, Rhynchophorus palmarum

Abstract

Rhynchophorus palmarum

p

p

Résumé

R. palmarum

p

p

Keywords:Keywords:Correspondence:Received: Accepted: Published:Copyright:

ISSN:

Cite this paper as:

Rhynchophorus palmarum Journal of Insect Science

Introduction

Rhynchosciara americ-ana

Erinnysis elloMacrotermes mulleri

Macrotermes bellicosus Macrotermes subhy-alinus

Diatraea saccaralis fabricius, Spodoptera frugiperda

Tenebrio molitor

Rhynchophorus palmarum

Materials and Methods

Chemicals

p

Biological materialR. palmarum

Elaeis guineensis

Enzyme samplesR. palmarum

Enzyme assays

pp

p

p

p

Protein assays

Purification procedures

Homogeneity and molecular weightdetermination

Temperature and pH optima

p

p

pH and temperature stabilities

Determination of kinetic parameters

pp

p

Effect of chemical agents

p

Transglucosylation reaction

R. palmarum

Results

Purification of beta-glucosidase

R. palmarum. p

Figure 1.Rynchophorus palmarum

Molecular weight

pH and temperature optimap

p

pH and temperature stabilities

Substrate specificity and kinetic parametersR. palmarum p

p

p

p

Figure 2.Rynchophorus palmarum

Figure 3.Rynchophorus palmarum

Table 1. Rhynchophorus palmarum

Purification steps Total protein (mg) Total activity (U) Specific activity (U/mg) Yield (%) Purification factor

Table 2.Rhynchophorus palmarum

Physicochemical properties Values

Hydrolyzis

Optimization of experimental conditions for transglucosylation reactions

Molecular weight

Effect of chemical agents

p

Effect of chemical agents on enzyme activity

p p

Transglucosylation reactionR. palmarum

R. palmarum

Discussion

R. palmarum

T. molitor

Diatraea saccharalis

M. subhyalinus

M. mulleri T. molitorFusobacterium

Thermus sp.

R. palmarump

Actinidia chinensisLeuconostoc mesenteroides et al

Tilapia

R. palmarum

p

Sclerotium rolfsii Aspergillusniger

Aspergil-lus

R. palmarum

R. palmarum

Aspergillus oryzaeAchatina achatina

Thermus thermophilus M. subhyalinus

R.palmarum

R. palmarum

R. palmarum

Escherichia coli A. oryzae

Figure 4.Rhynchophorus palmarum

R. palmarum

References

Diatrea saccharalis Insect Biochemistry and Molecular Biology

Biotechnology Advances

Current Opinion in Structural Biology

Methods in Enzymology

Electrophoresis

Thermus thermophilusGlycoconjugate

Journal

Rhynchosciara americana BiochemicalJournal

Tenebrio molitor Coleoptera Insect Biochemistryand Molecular Biology

Tenebrio molitor Journal of Insect Physiology

Tenebrio molitor Insect Biochemistry andMolecular Biology

Aspergillus oryzaeBiotechnology Letters

Thermus thermophilus AppliedMicrobiology and Biotechnology

Leuconostoc mesenteroides Journal ofMicrobiology

Candia sakeApplied Biochemistry and Biotechnology

Biochemical Journal

Biochem-ical Journal

Biochemical Society Transactions

Figure 5.Rhynchophorus palmarum

Figure 6.Rhynchophorus palmarum

Escherichia coli Canadian Journal of Biochemistry andCell Biology

Biotechnology

Macrotermes subhy-alinus Biotechnology Letters

Macrotermes subhyalinusIsoptera: Termitidae International Journal of Tropical Insect Science

Figure 7. Rhyncho-phorus palmarum

Table 3.

Oligosaccharide and polysaccharide substrates -glucosidase activity (%)

Table 4.Rhynchophorus palmarum

SubstrateKM

mMKcats-1

Kcat/KMmM-1s-1

p

Figure 8.Rhynchophorus palmarum

Periplaneta americanaBiotechnologie Agronomie Société et Environnement

Meth-ods of enzymatic analysis

Nature

Achatina achatinaBiochimica et Biophysica Acta

Flavobacteriummeningosepticum Biochemistry

Journal of Biology and Chem-istry

Spodoptera frugiperdaLepidoptera Insect Biochemistry and Molecular Biology

La symbiose termite-champignon chez Macrotermes bellicosus

Biochimica et BiophysicaActa

Journal of Japan Society of Food Science and Technology

Fusobacterium bacteriumBioscience Biotechnology and Biochemistry

European Journal of Biochemistry

Macrotermes mulleriTermitomyces sp Carbohydrates Research

Termotomyces clypeatus Biotechno-logy Progress

Trichoderma reesi Applied and Environmental Microbio-logy

Erinnyis ello Biochimica et Bio-physica Acta

Sclerotium rolfsii Archives of Bio-chemistry and Biophysics

Cana-dian Journal of Microbiology

Analytical Biochemistry

Tilapia Nippon Suisan Gakkaishi

Biochemical and Biophysical Re-search Communications

Pichia etchellsii Biochim-ica et Biophysica Acta

Aspergillus fumigatus Enzyme and Microbial Technology

Aspergillus nigerEuropean Journal of Biochemistry

Aspergillus terreus Applied and Environmental Microbiology