Www.soran.edu.iq Organic and biochemistry Preparation of amino acids. Reaction of the amino acids....
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www.soran.edu.iq Organic and biochemistry Preparation of amino acids. Reaction of the amino acids. Biochemical processes: Mechanism of enzyme action: chymotrypsin. 1
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Transcript of Www.soran.edu.iq Organic and biochemistry Preparation of amino acids. Reaction of the amino acids....
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Organic and biochemistry
Preparation of amino acids. Reaction of the amino
acids. Biochemical processes:
Mechanism of enzyme action: chymotrypsin.
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Preparation of amino acids: One of the most useful methods is the amination of α-halo acids. This is simply a nucleophilic substitution in which ammonia reacts with α-halo carboxylic acids. For example preparation of Alanine.
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Reaction of the amino acids: Amino acids undergo reactions characteristic of both their amine and carboxylic acid functional groups, we see the acylation is a typical reaction of the amino group. We will take the reaction of Glycine with the acetic anhydride.
Biochemical processes: Molecular biology:Mechanism of enzyme action. Chymotrypsin
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Enzyme, we see, are protein that acts as enormously effective catalysts for biological reactions. Let us examine the action of just one: Chymotrypsin, a digestive enzyme whose job is to promote hydrolysis of certain peptide links in protein. The sequence of the 245 amino acid residues in Chymotrypsin has been determined and, through x-ray analysis the conformation of the molecule is known:
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(A) Cleavage of proton with formation of acyl enzyme and liberation of one protein fragment.(b) Hydrolysis of acyl enzyme with regeneration of the enzyme and liberation of the other protein fragment. Look at the following equation:
![Qualitative tests of amino acids...Polar amino acids are more soluble in water[polar] than non-polar, due to presence of amino and carboxyl group which enables amino acids to accept](https://static.fdocument.org/doc/165x107/60abe5e424a07c772f79a096/qualitative-tests-of-amino-acids-polar-amino-acids-are-more-soluble-in-waterpolar.jpg)
