Product name: Pelargonidin 3-glucoside chloride OF ANALYSIS Date: 29.07.2014 CA No.: 2135/1 Product...
3
CERTIFICATE OF ANALYSIS Date: 29.07.2014 CA No.: 2135/1 Product name: Pelargonidin 3-glucoside chloride Basic Data Denotation: Pelargonidin 3-glucoside chloride Chemical name: 3-(β-D-Glucopyranosyloxy)-5,7- dihydroxy-2-(4-hydroxyphenyl)-1- benzopyrylium chloride Synonyms: 3-(Glucosyloxy)-4′,5,7-trihydroxy- flavylium chloride, Callistephin Batch No.: 14070702 CAS No.: [18466-51-8] Formula: C 21 H 21 ClO 10 Molecular weight: 468.84 Storage conditions: < -18 °C, dark place Shipment conditions: ambient conditions Source: Fragaria ananassa Stable until: July 2018 Date of production: July 2014 Article No.: A9650 Molecular Formula DETERMINATION SPECIFICATION RESULT Properties Appearance red powder conforms Solubility good soluble in Methanol, Water poorly soluble in 10 N Hydrochloric acid conforms Identity DAD UV-Spectrum Maxima at 270, 330, 430, 502 ± 3 nm λ max = 269, 331, 430, 504 nm; conforms Purity HPLC content of impurities at 270 nm and 502 nm: < 3.0 % 1.17 %, 1.18 %; conforms Assay HPLC (100 %-Method) >97.0 % at 270, 502 nm 98.83 %, 98.82 %; conforms RESULT: The product meets the requirements O HO OH OGlu OH Cl
Transcript of Product name: Pelargonidin 3-glucoside chloride OF ANALYSIS Date: 29.07.2014 CA No.: 2135/1 Product...
CERTIFICATE OF ANALYSIS Date: 29.07.2014
CA No.: 2135/1
Product name: Pelargonidin 3-glucoside chloride
Basic Data
Denotation: Pelargonidin 3-glucoside chloride Chemical name: 3-(β-D-Glucopyranosyloxy)-5,7- dihydroxy-2-(4-hydroxyphenyl)-1- benzopyrylium chloride
Synonyms: 3-(Glucosyloxy)-4′,5,7-trihydroxy- flavylium chloride, Callistephin
Batch No.: 14070702 CAS No.: [18466-51-8] Formula: C21H21ClO10 Molecular weight: 468.84 Storage conditions: < -18 °C, dark place Shipment conditions: ambient conditions Source: Fragaria ananassa Stable until: July 2018 Date of production: July 2014 Article No.: A9650
Molecular Formula
DETERMINATION SPECIFICATION RESULT
Properties Appearance red powder conforms
Solubility good soluble in Methanol, Water
poorly soluble in 10 N Hydrochloric acid
conforms
Identity
DAD UV-Spectrum Maxima at 270, 330, 430, 502 ± 3 nm λmax = 269, 331, 430, 504 nm;
conforms
Purity
HPLC content of impurities at 270 nm and 502 nm: < 3.0 %
1.17 %, 1.18 %; conforms
Assay
HPLC (100 %-Method) >97.0 % at 270, 502 nm 98.83 %, 98.82 %; conforms
RESULT: The product meets the requirements
OHO
OH
OGlu
OH
Cl
Analytical Report to the Certificate of Analysis (CA) CA No.: 2135/1
Date: 29.07.2014
Pelargonidin 3-glucoside chloride Page: 1 of 2
Batch No.: 14070702
HPLC-Analysis Column type: Eurospher C18, 100-5µ, 250 x 4.0 mm with integrated precolumn 5 x 4 mm Mobile Phase: Acetonitrile/Methanol/Trifluoroacetic acid 1 % in water (16/16//68, v/v/v) Sample solvent: Mobile Phase Flow rate: 1.00 mL/min Injection vol.: 5 µL Detection: DAD, 210-700 nm Column temp.: 20 °C
2: 270 nm, 4 nm Pk # Retention Time Area Area Percent Capacity factor Lambda Max
1 4.288 3053 0.06 1.21 269 2 4.864 1476 0.03 1.51 511 3 6.581 4782915 98.83 2.39 504 4 10.528 2283 0.05 4.43 495 5 12.277 1516 0.03 5.33 517 6 25.995 43000 0.89 12.40 506 7 32.117 5126 0.11 15.56 517
Totals 4839369 100.00
Minutes
0 10 20 30 40
0
100
200
300
400
500
600
700
4.4
80
4.8
96
5.9
41
6.5
81
10
.571
12.2
99
25.9
09
32.0
43
4: 502 nm, 4 nmPelargonidin-3-glucosid Ch.-B.: 14070702Pelargonidin-3-glc 14070702 RPmet2b-010.dat
Retention Time
Minutes
0 10 20 30 40
0
100
200
300
400
4.2
88
4.8
64
6.5
81
10.5
28
12.2
77
25
.995
32.1
17
2: 270 nm, 4 nmPelargonidin-3-glucosid Ch.-B.: 14070702Pelargonidin-3-glc 14070702 RPmet2b-010.dat
Retention Time
Analytical Report to the Certificate of Analysis (CA) CA No.: 2135/1
Date: 29.07.2014
Pelargonidin 3-glucoside chloride Page: 2 of 2
Batch No.: 14070702
HPLC-Analysis
4: 502 nm, 4 nm Pk # Retention Time Area Area Percent Capacity factor Lambda Max
1 4.480 641 0.01 1.31 501 2 4.896 2267 0.03 1.52 505 3 5.941 3940 0.05 2.06 500 4 6.581 7659300 98.82 2.39 504 5 10.571 4096 0.05 4.45 492 6 12.299 3940 0.05 5.34 499 7 25.909 69189 0.89 12.36 506 8 32.043 7024 0.09 15.52 515
Totals 7750397 100.00
DAD UV-Spectrum
Spectrum at time 6.58 min.
nm
300 400 500 600 700
mA
U
0
200
400
600
800
mA
U
0
200
400
600
800
6.58 minLambda max : 504 269 275 430 331Lambda min : 274 444 234 308 352
![Name Product Code Host Principal name Expected species ... · Bulk Product List Q1.2015.html[31/03/2015 14:47:12] Company Name Product Code Host Principal name Expected species cross-reactivity](https://static.fdocument.org/doc/165x107/5adc76277f8b9a8b6d8b9273/name-product-code-host-principal-name-expected-species-product-list-q12015html31032015.jpg)
