Polyclonal ANTIBODY Granulin - Proteintech · unique growth factor that plays an important role in...
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Granulin Polyclonal ANTIBODY Catalog Number:18410-1-AP Featured Product 5 Publications For Research Use Only www.ptglab.com Basic Information Catalog Number: 18410-1-AP Size: 30 μg/150 μl Source: Rabbit Isotype: IgG Purif ication Method: Antigen af f inity purif ication Immunogen Catalog Number: AG13178 GenBank Accession Number: BC000324 GeneID (NCBI): 2896 Full Name: granulin Calculated MW: 64 kDa Observed MW: 68 kDa, 70-88 kDa Recommended Dilutions: WB 1:500-1:1000 IP 0.5-4.0 ug f or IP and 1:500-1:1000 f or WB IHC 1:20-1:200 Applications Tested Applications: ELISA, WB, IHC, IP Cited Applications: IHC, WB Species Specif icity: human,mouse,rat Cited Species: human, mouse Positive Controls: WB : mouse lung tissue; A431 cells, HEK-293 cells, HeLa cells, mouse heart tissue, mouse kidney tissue, PC-3 cells, rat brain tissue, rat kidney tissue, THP-1 cells, U-937 cells IP : mouse kidney tissue; IHC : human skin cancer tissue; human kidney tissue, human skin cancer tissue Background Information GRN, also known as PGRN or PCDGF, is a cysteine-rich protein of 68.5 kDa that is typically secreted into a highly glycosylated 88 kDa f orm. PGRN is a unique growth f actor that plays an important role in cutaneous wound healing. It has an anti-inf lammatory ef f ect and promotes cell prolif eration. When PCDGF is degraded to several 6–25 kDa f ragments, called granulins (GRNs) by neutrophil proteases, a pro-inf lammatory reaction occurs. PGRN is widely expressed, particularly in epithelial cells, immune cells, neurons, and chondrocytes. High levels of PGRN expression have been reported in human cancers, and its expression is closely correlated with the development and metastasis of several cancers. The recent discovery that mutations in the gene encoding f or pro- granulin (GRN) cause f rontotemporal lobar degeneration (FTLD), and other neurodegenerative diseases leading to dementia, has brought renewed interest in progranulin and its f unctions in the central nervous system. Notable Publications Author Pubmed ID Journal Application Kun Huang 25626394 Immunology IHC Faisal Aziz 25527419 J Cancer Res Clin Oncol WB, IHC Faisal Aziz 25937205 Mol Med Rep Storage Storage: Store at -20ºC. Stable f or one year af ter shipment. Storage Buf f er: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary f or -20 º C storage T: 1 (888) 4PTGLAB (1-888-478-4522) (toll f ree in USA), or 1(312) 455-8498 (outside USA) E: [email protected] W: ptglab.com For technical support and original validation data f or this product please contact: This product is exclusively available under Proteintech Group brand and is not available to purchase from any other manufacturer.
Transcript of Polyclonal ANTIBODY Granulin - Proteintech · unique growth factor that plays an important role in...
GranulinPolyclonal ANTIBODYCatalog Number:18410-1-AP Featured Product 5 Publications
For Research Use Only
www.ptglab.com
Basic Information Catalog Number:18410-1-APSize:30 μg/150 μlSource:RabbitIsotype:IgGPurif ication Method:Antigen aff inity purif icationImmunogen Catalog Number:AG13178
GenBank Accession Number:BC000324GeneID (NCBI):2896Full Name:granulinCalculated MW:64 kDaObserved MW:68 kDa, 70-88 kDa
Recommended Dilutions:WB 1:500-1:1000 IP 0.5-4.0 ug for IP and 1:500-1:1000 for WBIHC 1:20-1:200
Applications Tested Applications:ELISA, WB, IHC, IPCited Applications:IHC, WBSpecies Specif icity:human,mouse,ratCited Species:human, mouse
Positive Controls:WB : mouse lung tissue; A431 cells, HEK-293 cells,HeLa cells, mouse heart tissue, mouse kidneytissue, PC-3 cells, rat brain tissue, rat kidneytissue, THP-1 cells, U-937 cellsIP : mouse kidney tissue;IHC : human skin cancer tissue; human kidneytissue, human skin cancer tissue
Background Information GRN, also known as PGRN or PCDGF, is a cysteine-rich protein of 68.5 kDa that is typically secreted into a highly glycosylated 88 kDa form. PGRN is aunique growth factor that plays an important role in cutaneous wound healing. It has an anti-inf lammatory effect and promotes cell proliferation. When PCDGFis degraded to several 6–25 kDa fragments, called granulins (GRNs) by neutrophil proteases, a pro-inf lammatory reaction occurs. PGRN is widely expressed,particularly in epithelial cells, immune cells, neurons, and chondrocytes. High levels of PGRN expression have been reported in human cancers, and itsexpression is closely correlated with the development and metastasis of several cancers. The recent discovery that mutations in the gene encoding for pro-granulin (GRN) cause frontotemporal lobar degeneration (FTLD), and other neurodegenerative diseases leading to dementia, has brought renewed interest inprogranulin and its functions in the central nervous system.
Notable Publications Author Pubmed ID Journal Application
Kun Huang 25626394 Immunology IHC
Faisal Aziz 25527419 J Cancer Res Clin Oncol WB, IHC
Faisal Aziz 25937205 Mol Med Rep
Storage Storage:Store at -20ºC. Stable for one year af ter shipment.Storage Buffer:PBS with 0.02% sodium azide and 50% glycerol pH 7.3.Aliquoting is unnecessary for -20ºC storage
T: 1 (888) 4PTGLAB (1-888-478-4522) (tollf ree in USA), or 1(312) 455-8498 (outsideUSA)
E: [email protected] W: ptglab.com
For technical support and original validation data for thisproduct please contact:
This product is exclusively available underProteintech Group brand and is not available topurchase from any other manufacturer.
Selected Validation Data
mouse lung tissue were subjected to SDS PAGEfollowed by western blot with 18410-1-AP(Granulin antibody) at dilution of 1:1000incubated at room temperature for 1.5 hours
IP Result of anti-Granulin (IP:18410-1-AP, 4ug;Detection:18410-1-AP 1:800) with mouse kidneytissue lysate 4000ug.
Immunohistochemistry of paraff in-embeddedhuman skin cancer slide using 18410-1-AP(Granulin antibody at dilution of 1:50
Immunohistochemistry of paraff in-embeddedhuman skin cancer slide using 18410-1-AP(Granulin antibody at dilution of 1:50
