MSU 300
4
Supplementary Figure 1. ER stress-induced secretion of mature IL- 1β by THP-1 cells is dependent on the NLRP3 inflammasome. MSU 300 MSU 300 MSU 300 MSU 300 Mock R837 15 shRNA : Scramble NLRP3 ASC Tunica 10 15 20 Mock R837 15 10 15 20 Mock R837 15 10 15 20 Mock R837 15 10 15 20 Tunica Tunica Tunica Caspase-1 µg/ml SN xT IL-1 pIL-1 pCasp1 ASC NLRP3 CHOP Sup. Fig. 1
description
Tunica. Tunica. Tunica. Tunica. Supplementary Figure 1. ER stress-induced secretion of mature IL-1β by THP-1 cells is dependent on the NLRP3 inflammasome. MSU 300. MSU 300. MSU 300. MSU 300. R837 15. 10. 15. 20. R837 15. 10. 15. 20. R837 15. 10. 15. 20. R837 15. 10. 15. 20. - PowerPoint PPT Presentation
Transcript of MSU 300
Supplementary Figure 1. ER stress-induced secretion of mature IL-1β by THP-1 cells is dependent on the NLRP3 inflammasome.
MS
U 3
00
MS
U 3
00
MS
U 3
00
MS
U 3
00
Moc
k
R83
7 15
shRNA : Scramble NLRP3 ASC
Tunica
10 15 20 Moc
k
R83
7 15
10 15 20 Moc
k
R83
7 15
10 15 20 Moc
k
R83
7 15
10 15 20
Tunica Tunica Tunica
Caspase-1
µg/ml
SN
xT
IL-1
pIL-1
pCasp1
ASC
NLRP3
CHOP
Sup. Fig. 1
Supplementary Figure 2. ER stress-induced activation of the NLRP3 inflammasome is independent of TLR and RLR signaling.
BF
A 0
.1
BF
A 0
.1
MS
U 1
50 µ
g/m
l
MS
U 1
50 µ
g/m
l
CB
A
moc
k
R83
7 15
Tun
ica
15
µg/ml
SN
xT
shRNA : Scramble MyD88m
ock
R83
7 15
Tun
ica
15
IL-1
pIL-1
pCasp1
MyD88
MS
U 1
50 µ
g/m
l
Moc
k
Tha
psi 1
0 µ
M
Tun
ica
20 µ
g/m
l
Trif+/+ Trif-/-
Tun
ica
10 µ
g/m
l
Tha
psi 5
µM
MS
U 1
50 µ
g/m
l
Moc
k
Tha
psi 1
0 µ
M
Tun
ica
20 µ
g/m
l
Tun
ica
10 µ
g/m
l
Tha
psi 5
µM
SN
xT
IL-1
pIL-1
pCasp1
Casp1
pIL-1
pCasp1
CHOP
Moc
k
Tha
psi 1
0 µ
M
Tun
ica
20 µ
g/m
l
Mavs+/+ Mavs-/-
Tun
ica
10 µ
g/m
l
Tha
psi 5
µM
Moc
k
Tha
psi 1
0 µ
M
Tun
ica
20 µ
g/m
l
Tun
ica
10 µ
g/m
l
Tha
psi 5
µM
SN
xT
pIL-1
IL-1
pCasp1
Casp1
pIL-1
pCasp1
CHOP
Sup. Fig. 2
Supplementary Figure 3. IRE1α shutdown in shRNA-expressing THP-1 cells.
shIR
E1
#1
shIR
E1
#2
shIR
E1
#3
Em
pty
vect
or
xT
IRE1α
Tubulin
Sup. Fig. 3
Supplementary Figure 4. TRAF2 and ASK1 are dispensable for ER stress-induced NLRP3 inflammasome activation.
B
A
Moc
k
ScrambleshRNA :
MS
U 3
00 µ
g/m
l
R83
7 20
µg/
ml
Tun
ica
10 µ
g/m
l
Moc
k
MS
U 3
00 µ
g/m
l
R83
7 20
µg/
ml
Tun
ica
10 µ
g/m
l
Moc
k
MS
U 3
00 µ
g/m
l
R83
7 20
µg/
ml
Tun
ica
10 µ
g/m
l
TRAF2 #1 TRAF2 #2
xT
SN IL-1
pIL-1
TRAF2M
SU
300
µg/
ml
Moc
k
Tha
psi 5
µM
Tun
ica
10 µ
g/m
l
Ask1+/+ Ask1-/-
Tun
ica
5 µ
g/m
l
Tha
psi 2
.5 µ
M
MS
U 3
00 µ
g/m
l
Moc
k
Tha
psi 5
µM
Tun
ica
10 µ
g/m
l
Tun
ica
5 µ
g/m
l
Tha
psi 2
.5 µ
M
xT
SN
pIL-1
pCasp1
pCasp1
Casp1
Sup. Fig. 4
